RESUMO
DNA sequence d(TTAGGG) spontaneously forms all-parallel G-quadruplex DNA in the presence of KV We have shown that G-quadruplex DNAs formed from the related sequences undergo dimerization through the intermolecular end-to-end stacking of the 3'-terminal G-quartets. 2' 3 We found that the coexistence of G-quadruplex DNAs formed from two different sequences results in the formation of "hetero-dimer", in addition to "homo-dimer". Thermodynamic characterization of the dimerization process revealed a compensatory relationship between the enthalpy and entropy changes of the process.
Assuntos
DNA/química , Quadruplex G , Oligodesoxirribonucleotídeos/química , Telômero/química , Dimerização , Humanos , Modelos Moleculares , Ressonância Magnética Nuclear Biomolecular , TermodinâmicaRESUMO
DNA fragment, d(TTAGGG), forms all-parallel G-quadruplex in the presence of K(+).(1) We found that G-quadruplex d(TTAGGG) dimerizes through end-to-end stacking of the 3'-terminal G-quartets.(2) In this study, we report the effects of Mg(2+) and Ca(2+) on the dynamics and thermodynamics of the dimerization.
