Effects of Sixty-Minute Race-Pace Running on Cardiac Stress Biomarkers in Recreational Distance Runners.

Sudden cardiac death (SCD) in athletes is generally rare, but a serious complication of cardiovascular events during exercise. Although regular intensive physical exercise is thought to be a key to a healthy life, unsuspected pathologies might lead to SCD during or after physical activity. Cardiac dysfunction and elevated cardiac markers have been reported after prolonged exercise. We sought to clarify the cardiac marker levels and hydration status in healthy, middle-aged male subjects for 24 hours after running sixty-minute at race-pace. The participants were 47.4±1.7 years old, had peak oxygen consumption of 47.1±1.2ml/kg/min, and regularly running 70.5±6.4km/week. Blood biomarkers were performed before, immediately after, at the fourth and twenty-fourth hours after running. Compared to initial values, creatine kinase (before:161.2±22.5U/L, 24 hours after:411.9±139.7U/L, p<0.001) and CK-MB (before:4.3±0.7ng/ml, 24 hours after:10.1±3.0ng/ml, p<0.001) were significantly elevated immediately after running and remained significantly high for 24 hours. In addition, Troponin-I (before:5.0±1.1ng/l, 4 hours after:81.5±29.9ng/l, p<0.001) and NT-proBNP (before: 31.2±5.3pg/ml, immediately after: 64.4±8.5pg/ml, p<0.01) were significantly elevated immediately after running and returned to baseline levels in 24 hours. The sixty-minute running caused significant dehydration, but athletes were rehydrated at the 4th hour in their voluntary hydration behavior. As the individual data were analyzed, it was interesting to see that some of the athletes had critical biomarker levels without any cardiac symptom. Our findings indicate that race-pace sixty-minute running may induce a possible transient silent myocardial injury in apparently healthy master runners. Detailed pre-participation screening of these athletes may be necessary to reduce the risk of SCD.

Living, training and playing in the heat: challenges to the football player and strategies for coping with environmental extremes.

Dehydration and hyperthermia both, if sufficiently severe, will impair exercise performance. Dehydration can also impair performance of tasks requiring cognition and skill. Body temperature may exceed 40 °C in competitive games played in hot weather, but limited data are available. Football played in the heat, therefore, poses a challenge, and effects on some aspects of performance become apparent as environmental temperature increases above about 12-15 °C. Prior acclimatization will reduce the impact of high environmental temperatures but provides limited protection when humidity is also high. Ingestion of fluids is effective in limiting the detrimental effects on performance: drinks with added carbohydrate and electrolytes are generally more effective than plain water and drinks may be more effective if taken cold than if taken at ambient temperature. Pre-exercise lowering of body temperature may aid some aspects of performance, but the efficacy has not been demonstrated in football.

Hydration and sweating responses to hot-weather football competition.

During a football match played in warm (34.3 ± 0.6 °C), humid (64 ± 2% rh) conditions, 22 male players had their pre-match hydration status, body mass change, sweat loss and drinking behavior assessed. Pre-match urine specific gravity (1.012 ± 0.006) suggested that all but three players commenced the match euhydrated. Players lost 3.1 ± 0.6 L of sweat and 45 ± 9 mmol of sodium during the 90-min match and replaced 55 ± 19% of their sweat losses and hence by the end of the game were 2.2 ± 0.9% lighter. The water volume consumed during the game was highly variable (1653 ± 487 mL; 741-2387 mL) but there was a stronger relationship between the estimated pre-game hydration status and water volume consumed, than between sweat rate and water volume consumed. In a second match, with the same players 2 weeks later in 34.4 ± 0.6 °C, 65 ± 3% rh, 11 players had a sports drink available to them before and during the match in addition to water. Total drink volume consumed during the match was the same, but approximately half the volume was consumed as sports drink. The results indicate that substantial sweat water and electrolyte losses can occur during match play in hot conditions and a substantial water and sodium deficit can occur in many players even when water or sports drink is freely available.

Effect of hot environmental conditions on physical activity patterns and temperature response of football players.

Heat stress may contribute to decreased match performance when football is played in extreme heat. This study evaluated activity patterns and thermal responses of players during soccer matches played in different environmental conditions. Non-acclimatized soccer players (n=11, 20±2 years) played two matches in conditions of moderate heat (MH) and high heat (HH) index. Core temperature (T(c) ) and physical performance were measured using a telemetric sensor and a global positioning system, respectively. The average ambient temperature and relative humidity were MH 34±1 °C and 38±2%; HH 36±0 °C and 61±1%. Peak T(c) in the MH match was 39.1±0.4 °C and in the HH match it was 39.6±0.3 °C. The total distance covered in the first and second halves was 4386±367 and 4227±292 m for the MH match and 4301±487 and 3761±358 m for the HH match. Players covered more distance (P<0.001) in the first half of the HH match than in the second half. In football matches played at high environmental temperature and humidity, the physical performance of the players may decrease due to high thermal stress.

Effect of gonadotrophin dose on oocyte retrieval in superovulated BALB/c mice.

Mice are commonly used animal models in reproductive and developmental research. In order to get satisfying results from such experiments, large numbers of ova must be available and this can be achieved by using various ovulation induction protocols. To obtain an optimal response from these stimulation protocols, parameters such as breeding-housing conditions of the animal strains, the best age for superovulation, and type and dose of gonadotrophins must be optimized. The aim of this study was to investigate the impact of exogenous stimulation with increasing amounts of gonadotrophins on the number and quality of oocytes/pre-embryos recovered from outbred BALB/c mice. A dose-response analysis was performed by stimulating prepubescent (21- to 25-day-old) and sexually mature (6 to 8 weeks old) female mice with hMG, which contains equal amounts of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The stimulation dose contained 5, 10, 15, 20, 25 or 30 IU of FSH/LH. The effect of increasing stimulation was assessed by monitoring the number and maturity of ova recovered from the tubes. The data were analyzed by using a one-way Anova test and student t-test. Increasing stimulation doses in the prepubescent females resulted in an increased number of ova. A maximum of 55 ova per mouse was reached when stimulating with 20 IU of FSH/LH; higher stimulation doses showed no further increase in oocyte recovery. In the prepubescent group, a maximal number of recovered mature ova was reached with 15 IU of FSH/LH. In the sexually mature female group, 20 IU of FSH/LH gave the best quantitative and qualitative results. Positive effects of copulation on the number and maturity of oocytes in all induction doses were more evident in the prepubescent females and these parameters were significantly more improved (P < 0.05) in this group when compared to the pubertal females. Our findings led to the conclusion that ovulation induction of prepubescent outbred BALB/c mice with 15 IU FSH/LH and sexually mature ones with 20 IU FSH/LH give the best results in terms of oocyte number and maturity.