RESUMO
Substance P (SP) is an ll-amino acid neuropeptide of the tachykinin family. This study investigated the preparation of an SP analog labeled with 99mTc for imaging SP receptor positive tissue (inflammatory diseases and neoplasms with increased expression of SP receptors). High specific activity 99mTc-SP was prepared using the l-imino-4-mercaptobutyl (IMB) group as a bifunctional chelator. Biological distribution in mice showed increased 99mTc-SP uptake in the salivary glands (which have a high concentration of SP receptors), but this activity was displaced in animals pre-treated with excess non-radioactive SP. These data show that the uptake of 99mTc-SP is receptor specific. IMB derivatization at the N-terminal location is suitable for labeling of SP analogues with 99mTc. Synthetic analogues of SP labeled with 99mTc may be an attractive alternative to 111In labeled molecules and merit further investigation.
Assuntos
Compostos Radiofarmacêuticos/síntese química , Substância P/análogos & derivados , Tecnécio , Animais , Quelantes , Estabilidade de Medicamentos , Técnicas In Vitro , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Cintilografia , Receptores da Neurocinina-1/metabolismo , Glândulas Salivares/diagnóstico por imagem , Glândulas Salivares/metabolismo , Substância P/síntese químicaRESUMO
Spinal cord lesions are the result of many etiological factors and are associated with motor, sensorial and autonomic dysfunctions. The subjects evaluated were a total of 217 paraplegics and quadriplegics who had been enrolled in a rehabilitation program during the last five years conducted by the Department of Physical Medicine and Rehabilitation of the Hacettepe University Faculty of Medicine. Forty-three of these patients were in the pediatric age group. In this clinical trial, the patients with spinal cord injuries were examined according to their age, sex, etiological factors and functional status. Frankel's scale was used for functional assessment. The mean age of patients was 9.19 +/- 4.19 years and varied between 1-16 years. The results of our classification according to etiological factors were as follows: Nineteen patients with tumors (44.1%), nine patients with infections (20.93%), five patients with congenital abnormalities (11.63%), five patients with vascular lesions (11.63%), four patients with trauma (9.30%), and one patient with a degenerative central nervous system disorder (2.32%). The effect of the rehabilitation program on the functional status of the patients is discussed.
