Effect of Oltipraz on urethral healing: An experimental study.

BACKGROUND: We aimed to examine the oral and topical effect of Oltipraz (OPZ) on fibrosis and healing after urethra injury in a rat model. METHODS: In all, 33 adult Sprague-Dawley rats were divided randomly into 5 different groups: sham, urethral injury group (UI), oral Oltipraz treatment group for 14 days after urethral injury (UI+oOPZ), intraurethral Oltipraz treatment group for 14 days after urethral injury (UI+iOPZ) and only intraurethral Oltipraz treatment for 14 days without urethral injury (sham+iOPZ). Pediatric urethrotome blade was used to create the urethral injury model for the injury groups (UI, UI+oOPZ and UI+iOPZ). After 14 days of treatment, all rats were sacrificed after penectomy under general anesthesia. Urethral tissue was evaluated histopathologically for congestion, inflammatory cell infiltration and spongiofibrosis, and immunohistochemically for transforming growth factor Beta-1 (TBF) and vascular endothelial growth factor receptor2 (VEGFR2). RESULTS: The congestion score was not statistically significantly different between the groups. Spongiofibrosis was distinctive in UI group and OPZ given groups. Inflammation and spongiofibrosis score were statistically significantly higher in the sham+iOPZ group compared to the sham group (P<0.05). VEGFR2 and TGF Beta-1 scores were statistically significantly higher in the sham+iOPZ group compared to the sham group (P<0.05). We did not find beneficial effect of OPZ on urethral healing. We found the harmful effect of intraurethral administration of OPZ in the group without urethral injury in compared to sham. CONCLUSIONS: According to our results, we cannot suggest OPZ in the treatment of urethral injury. Future studies in this area are needed.

An unusual case of oropharyngeal mature teratoma in a kitten.

Background: Extragonadal teratoma in the head and neck region is unusual in veterinary medicine. So far, only one case of oropharyngeal teratoma has been reported in a cat. Case description: In this report, a 5-month-old kitten was brought to the clinic with the complaint of difficulty breathing, food intake, and oropharyngeal mass. In clinical examination, there was a large mass, protruding from the localization included left buccal mucosa, soft palate, and extending into the oropharynx but no cranial extension was observed in CT scans. The mass was completely resected. Macroscopically, the mass measured 4.5 × 6.5 cm2 and contained solid and cystic areas associated with soft and hard tissue components. Findings/treatment and outcome: Histopathology revealed an extragonadal solid-cystic (grade 0) oropharyngeal mature teratoma containing structures from endoderm, mesoderm, and ectoderm. After an 8-month follow-up control period, no signs of recurrence were observed. Conclusion: The case was deemed worthy of being presented with its clinical, radiological, and pathological findings, and complete resection was curative for mature teratoma.

Morphology of Naturally-Occurring Tuberculosis in Cattle Caused by Mycobacterium caprae.

This study describes the pathomorphological alterations of bovine tuberculosis through gross and histopathological examinations, assessment of the distribution of lesions and the demonstration of mycobacteria. Samples from lungs, liver, small intestine, their regional lymph nodes and retropharyngeal lymph nodes were collected from 84 cattle with tuberculosis from the Allgäu, Germany. Organs were evaluated grossly, histopathologically and by transmission electron microscopy. Mycobacteria and mycobacterial antigens were demonstrated using acid-fast staining and immunohistochemistry (IHC). Bacteriological tests revealed Mycobacterium caprae in all animals. Gross alterations were classified into five patterns (I to V) with an additional pattern of acute exudative pulmonary inflammation (pattern VI). Histological lesions were classified into four types (1-4) with additional lesions occurring in lungs only. Acid-fast staining revealed a low number of bacteria in all tissues, while IHC showed comparatively more mycobacterial antigens within the lesions and also at their periphery. The alimentary tract (68%) was the main portal of entry followed by an aerosol infection (19%). It was assumed that the observed lesions reflect a continuous primary period of infection; there were no lesions typical of a secondary (post-primary) period, as reported in man and also described in the older literature on bovine tuberculosis. The broad spectrum of changes described formerly was not observed in the present cases and the route of infection and nature of acid fast staining showed differences when compared with previous studies of naturally-occurring bovine tuberculosis.