Role of cell wall-degrading enzymes in pathogenicity of Fusarium oxysporum.

Fusarium oxysporum invades its host plants through the roots and colonizes the vascular system. It produces a great variety of cell-wall degrading enzymes (CWDE), such as cellulases, xylanases, pectinases and proteases. Our group has purified and characterized an endopolygalacturonase (PG1), two exopolygalacturonases (PG2 and PG3), an endoxylanase (XYL1) and an endo pectatelyase (PL1). We have isolated the following CWDE-encoding genes: pg1, pgx4, pg5, xyl2, xyl3, prt1 and pl1. Gene expression in different culture conditions has been determined by Northern analysis. The occurrence of these genes in different formae speciales has been analyzed by Southern analysis and PCR. All these genes are expressed during different stages of the interaction with the host plant indicating a possible role in pathogenesis. At present, targeted gene disruption is being carried out, in order to determine the role of each gene in the pathogenicity process.

Prueba de tabla basculante para desencadenamiento de síncopes cardíacos mediados por el Sistema Nervioso Neuro-Vegetativo / Half-full test of tilted table for outbreak of síncopes cardiac by the Neuro-vegetative nervous system

Analiza que la prueba de tabla basculante o Head Up Tilt Test (HUT) es un examen de realización simple que permite valorar síncopes cardíacos mediados por el sistema nervioso autónomo. (SNA). Hemos realizado entre jun. 1995 y ju. 1997 n=44 estudios, 18 hombres de edad promedio 35 años y 26 mujeres de edad promedio 32,3 años. El test se reveló positivo en n=25, (56xciento) pacientes. A partir de la clasificación de Tulesius hemos clasificado la respuestas en 16 vasovagales (36.4xciento) de N=44.9 (20.5xciento) hipoadrenérgicos de n=44. En 6 pacientes (13.6xciento) se obtuvo una reacción de tipo hipotensión ortostática y 13 pacientes (29.5xciento) fueron normales. Dentro de este grupo 3 pacientes (6.8xciento) presentaron una reacción hiperadrenérgica...