Determination of basal bone mineral density in the femur bones of male and female Wistar rats.

Changes in bone mineral content of calcium (Ca), phosphorous (P), magnesium and potassium for male and female Wistar rats during their development from 3 weeks old to adulthood (27 weeks old) were measured. Bone mineral content was related to areal bone mineral density (BMD) which was measured in vivo at the femoral neck using a calibrated X-ray transmission system to obtain basal curves as a function of the age of the specimen. Diagnostic curves were built to determine low BMD (osteopaenia) and osteoporosis in female rats fed a Ca-depleted diet (50%) based on the obtained data and the criteria established by the World Health Organization. Bone mineral content is directly related to sex and age, but P did not change throughout the experimental period. P content did not exhibit significant changes with growing, while Ca was greatest in male rats, producing significant differences in the Ca:P ratio. Male rats reach the Ca:P ratio peak before female rats. However, areal BMD does not follow the same trend. On the other hand, osteoporosis produced a 45% decrease in this parameter for young and mature adults. These results make Z-score values available to diagnose bone-mass losses and hence the possibility of improving the conditions of non-contact measurement of BMD in vivo. This technique can be used for future experiments with Wistar rats.

Chemical analysis of nutritional content of prickly pads (Opuntia ficus indica) at varied ages in an organic harvest.

Opuntia ficus indica, also known as prickly pads, are an important part of the human diet and are also used as forage for livestock. This is an interesting vegetable due the environmental conditions in which it grows and its resistance to climatic extremes; however, little is known about its nutritional properties, especially in the later stages of maturity. The objective of this study was to determine the composition of organic prickly pads (Opuntia ficus indica) at differing stages of growth maturity. Chemical proximate analysis and mineral constituent analysis at different maturation stages were carried out in this investigation. As a result, older prickly pads were found to be an important source of nutritional components such as calcium.

Early undernourishment interferes with the maternal aggressive response triggered by an intruder entering the homing cage.

The maternal aggressive response (MAR) against intruders is temporarily expressed during lactation in association with the rearing and protection of offspring to promote their survival and growth in the nest. This normal component of maternal behaviour requires both the hormonal changes occurring at the end of pregnancy and the presence of pups for its establishment. Because early food restriction in the rat results in long-term maternal deficiencies, we analysed in Wistar rats the effects of perinatal undernutrition on the MAR to an intruder at days 1, 4, and 8 postpartum. The data showed that undernourished dams exhibited significant reductions of sniffing frequency on days 4 and 8 and significant increases of biting on day 4 and of lateral attacks on postpartum days 4 and 8. The finding of an altered MAR during the lactating period may be relevant for the survival and long-term behavioural development of the progeny.

Early undernutrition decreases the number of neurons in the locus coeruleus of rats.

The effects of perinatal undernutrition on the number of neurons and apoptotic cells of the locus coeruleus (LC) of female and male rats at postpartum days 7, 12, 20, 30 and 60 were studied. Undernutrition reduces the number of neurons in both sexes without affecting cell death, as indicated by the ratio of apoptotic cells to neurons. The data suggest that in the undernourished groups lower rates of neurogenesis and proliferation (neurogenetic/proliferation rates) might avoid these animals achieving the number of LC neurons as in the control subjects. Although food restriction in both sexes apparently provokes the loss of cells, the effect does not appear to be equal in females and males, as shown by post weaning food rehabilitation. The results suggest that severe food deprivation may interfere with the ontogenetic processes underlying neuronal differentiation of the LC. Morphological damage in the LC due to undernutrition might alter the physiology of sexual and/or feeding behaviours in which this structure is implicated.

Undernutrition and food rehabilitation effects on the locus coeruleus in the rat.

Perinatal nutrition plays a fundamental role on the morphological organization and function of a number of brain stem structures. Because little is known of the effects of perinatal undernutrition upon sexually dimorphic structures underlying reproductive behavior, the locus coeruleus morphology of 60-day-old male and female Wistar rats was analyzed. Perinatal food deprivation until weaning significantly decreased the volume and neuronal number of locus coerulus in male and female rats, while nutritional rehabilitation ameliorated these alterations in males but not in females. Data suggest that perinatal undernutrition interferes with the neuroendocrine mechanisms underlying the establishment of sex differences of the locus coeruleus.

A paradigm of undernourishing and neonatal rehabilitation in the newborn rat.

Perinatal undernutrition as a deficiency of nutrient availability, affects body and brain developmental processes and promotes recurrent health problems. Thus, altered mother-litter bonds and deficient environmental interactions may interfere with the brain pluripotential capabilities of the newborn. To gather information concerning the mechanisms underlying perinatal undernutrition we designed a paradigm of undernutrition and neonatal rehabilitation in the rat. An underfed group came from pregnant Wistar rats fed with 50% of the diet from G6 to G12 and with 60% from G13 until G21. After birth, pups were daily undernourished during 12 h daily by rotating a pair of lactating well-nourished dams which had one of their nipples subcutaneously ligated. The rehabilitated animals were undernourished pups neonatally fed by a pair of normally lactating dams. Controls received plenty of food during the pre- and neonatal periods. Pups were sacrificed at 12, 20 and 30 days of age. Perinatal underfeeding significantly reduced body and brain weights and neuronal morphometric parameters. Normal neonatal feeding in the newborn ameliorated the damages associated to food deprivation. The current undernourishing paradigm may be helpful to assess brain development alterations, as well as to study the compensatory mechanisms associated to salutary epigenetic influences.

Neonatal nutritional rehabilitation of morphological features in facial motoneurons altered by prenatally food deprivation in the rat.

Undernutrition alters facial motoneurons development. Here, we evaluated the plastic characteristics of facial motoneurons in response to neonatal rehabilitation. Prenatally undernourished rat pups derived from pregnant rats fed with 50% of the diet from gestational days G6 to G12, and with 60% of food from G13 to G21 and rehabilitated after birth by a pair of control lactating foster dams. Morphological features of 640 Golgi-Cox impregnated motoneurons from 12, 20 and 30 days old pups were analyzed. Neonatal food rehabilitation increased the number and extension of the dendritic branches (main neuronal reception area) without altering significantly soma measurements (main neuronal firing area), suggesting that food and sensory maternal disponibility after birth ameliorates the damage due to prenatal deprivation. Findings showed that neonatal rehabilitation in addition to intense maternal polysensorial stimuli from the foster mothers partially diminished the morphological alterations associated to prenatal undernutrition, and provide evidence of plastic properties of facial motoneurons to the influence of environmental cues.

Efectos del estrés prenatal sobre la ramificación neuronal en la corteza visual de ratas neonatas / Prenatal stress of effects on neuronal branching in the visual cortex of the pup rats

Se evaluó el efecto del estrés por inmovilización aplicado a ratas gestantes, sobre la morfología de las neuronas piramidales en la corteza visual de machos descendientes de dichas hembras, a los 14 y 21 días posnatales. Ratas hembra de la cepa Wistar fueron sometidas a estrés por inmovilización forzada durante toda la gestación, en periodos que variaron entre 2 y 6 horas por día. En neuronas del área visual, impregnadas con el método de Golgi se cuatificó el grado de ramificación dendrítica. Los resultados muestran disminución de ramificaciones dendríticas sobre todo en ratas de 21 días. Las deficiencias en la capacidad de aprendizaje y comportamiento adaptativo observadas en animales descendientes de madres sometidas a estrés durante la gestación podrían explicarse con base a estos resultados