Oilseeds in diets of fattening lambs / Oleaginosas em dietas para cordeiros em engorda

An experiment was conducted to compare different oilseeds in replacement of ground corn in diets of fattening lambs, evaluating performance, apparent diet digestibility, ingestive behavior, and carcass traits. Twenty-four crossbred lambs, three months old and with initial body weight 25.97 ± 0.75 kg, were kept in individual pens. The animals received one of the following diets: ‘control (no oilseeds, 3.11% Ether Extract - EE), ‘cottonseed (6.14% EE), ‘sunflower (6.33% EE), and ‘soybean (6.30% EE). All diets contained 23.63% of coast cross hay and 76.37% of concentrate. After 84 days in feedlot, the animals were slaughtered and the carcasses were evaluated. The dry matter intake (DMI) and daily weight gain (DWG) of animals fed with the control diet was higher than sunflower (P≤0.05). Final body weight and carcass weight were higher (P≤0.05) in the control (42.42 kg and 19.27 kg, respectively) than for sunflower diet (34.85 kg and 16.05 kg, respectively). Neutral detergent fiber (NDF) digestibility was reduced (P≤0.05) when the animals received cottonseed diet (50.34%) in comparison to soybean (71.07%), sunflower (67.07%) and control (66.50%). NDF digestibility for soybean, sunflower and control diets did not differ from each other. Lower (P≤0.05) EE digestibility was observed for the control diet (73.67%). There were no differences (P≥0.05) in the ingestive behavior, however, the addition of cottonseed or sunflower negatively affected (P≤0.05) rumination efficiency of NDF. Sunflower should not be included in high concentrate diets for fattening lambs.(AU)

Um experimento foi conduzido para comparar diferentes oleaginosas em substituição ao milho moído na dieta de cordeiros em engorda, avaliando o desempenho, a digestibilidade aparente da dieta, o comportamento ingestivo e as características de carcaça. Vinte e quatro cordeiros cruzados, com 3 meses de idade e peso corporal inicial de 25,97 ± 0,75 kg, foram mantidos em baias individuais. Eles receberam uma das seguintes dietas ou tratamentos: ‘controle (sem grãos de oleaginosas, 3,11% de Extrato Etéreo - EE), ‘caroço de algodão (6,14% EE), ‘girassol (6,33% EE) e ‘soja (6,30% EE). Todas as dietas continham 23,63% de feno de coast cross e 76,37% de concentrado. Após 84 dias de confinamento, os animais foram abatidos e as carcaças avaliadas. O consumo de matéria seca e ganho de peso diário dos animais alimentados com a dieta controle foi maior daqueles alimentados com girassol (P≤0,05). O peso corporal final e o peso da carcaça foram maiores (P≤0,05) para aqueles alimentados com a dieta controle (42,42 kg e 19,27 kg, respectivamente) que aqueles da dieta com girassol (34,85 kg e 16,05 kg, respectivamente). A digestibilidade da Fibra em Detergente Neutro (FDN) foi reduzida (P≤0,05) quando os animais receberam caroço de algodão (50,34%) comparado à soja (71,07%); para as dietas com girassol (67,07%) e controle (66,50%) não houve diferenças das demais. Menor (P≤0,05) digestibilidade do extrato etéreo foi observada para a dieta controle (73,67%). Não houve diferenças (P≥0,05) no comportamento ingestivo, no entanto, a inclusão de caroço de algodão ou girassol afetou negativamente (P≤0,05) a eficiência de ruminação da FDN. Grãos de girassol não devem ser incluídos em dietas de alto concentrado para cordeiros em engorda.(AU)

Oilseeds in diets of fattening lambs / Oleaginosas em dietas para cordeiros em engorda

An experiment was conducted to compare different oilseeds in replacement of ground corn in diets of fattening lambs, evaluating performance, apparent diet digestibility, ingestive behavior, and carcass traits. Twenty-four crossbred lambs, three months old and with initial body weight 25.97 ± 0.75 kg, were kept in individual pens. The animals received one of the following diets: ‘control’ (no oilseeds, 3.11% Ether Extract - EE), ‘cottonseed’ (6.14% EE), ‘sunflower’ (6.33% EE), and ‘soybean’ (6.30% EE). All diets contained 23.63% of coast cross hay and 76.37% of concentrate. After 84 days in feedlot, the animals were slaughtered and the carcasses were evaluated. The dry matter intake (DMI) and daily weight gain (DWG) of animals fed with the control diet was higher than sunflower (P≤0.05). Final body weight and carcass weight were higher (P≤0.05) in the control (42.42 kg and 19.27 kg, respectively) than for sunflower diet (34.85 kg and 16.05 kg, respectively). Neutral detergent fiber (NDF) digestibility was reduced (P≤0.05) when the animals received cottonseed diet (50.34%) in comparison to soybean (71.07%), sunflower (67.07%) and control (66.50%). NDF digestibility for soybean, sunflower and control diets did not differ from each other. Lower (P≤0.05) EE digestibility was observed for the control diet (73.67%). There were no differences (P≥0.05) in the ingestive behavior, however, the addition of cottonseed or sunflower negatively affected (P≤0.05) rumination efficiency of NDF. Sunflower should not be included in high concentrate diets for fattening lambs.

Um experimento foi conduzido para comparar diferentes oleaginosas em substituição ao milho moído na dieta de cordeiros em engorda, avaliando o desempenho, a digestibilidade aparente da dieta, o comportamento ingestivo e as características de carcaça. Vinte e quatro cordeiros cruzados, com 3 meses de idade e peso corporal inicial de 25,97 ± 0,75 kg, foram mantidos em baias individuais. Eles receberam uma das seguintes dietas ou tratamentos: ‘controle’ (sem grãos de oleaginosas, 3,11% de Extrato Etéreo - EE), ‘caroço de algodão’ (6,14% EE), ‘girassol’ (6,33% EE) e ‘soja’ (6,30% EE). Todas as dietas continham 23,63% de feno de coast cross e 76,37% de concentrado. Após 84 dias de confinamento, os animais foram abatidos e as carcaças avaliadas. O consumo de matéria seca e ganho de peso diário dos animais alimentados com a dieta controle foi maior daqueles alimentados com girassol (P≤0,05). O peso corporal final e o peso da carcaça foram maiores (P≤0,05) para aqueles alimentados com a dieta controle (42,42 kg e 19,27 kg, respectivamente) que aqueles da dieta com girassol (34,85 kg e 16,05 kg, respectivamente). A digestibilidade da Fibra em Detergente Neutro (FDN) foi reduzida (P≤0,05) quando os animais receberam caroço de algodão (50,34%) comparado à soja (71,07%); para as dietas com girassol (67,07%) e controle (66,50%) não houve diferenças das demais. Menor (P≤0,05) digestibilidade do extrato etéreo foi observada para a dieta controle (73,67%). Não houve diferenças (P≥0,05) no comportamento ingestivo, no entanto, a inclusão de caroço de algodão ou girassol afetou negativamente (P≤0,05) a eficiência de ruminação da FDN. Grãos de girassol não devem ser incluídos em dietas de alto concentrado para cordeiros em engorda.

Economic values and selection index in different Angus-Nellore cross-bred production systems.

Bioeconomic models were developed to calculate economic values (EV) for economically important traits in beef cattle, to evaluate the impact of these traits on production profitability, to assess possible market changes with a payment system and to develop economic selection indexes for Angus cattle for two production systems. Two beef cattle production systems were simulated as follows: a cow-calf cycle (CC) and a complete cycle (CoC). Following selection, positive changes in the EV were observed. In the CC, each 1.0% increment in weaning weight (WW), weaning rate (WR) and pregnancy rate (PR) resulted in increases in US$ 1.30, US$ 3.68 and US$ 3.55 per cow/year in profit, respectively. In the CoC, EV of US$ 1.01, US$ 1.79, US$ 1.19, US$ 1.34, US$ 6.84 and US$ 7.86 per cow/year were obtained for WW, year weight, yearling weight, final weight, WR and PR, respectively. The payment system for carcass quality showed that the scenario considering that 100% of the animals displayed uniform carcasses exhibited the highest EV and was considered optimal. Considering the sensitivity analysis, the price paid per animal was the factor that most affected the EV in both systems. The selection indexes obtained may be used in similar production systems, and the use of EV and selection indexes are important tools for any production system with positive change in profit after selection.

B4GALT1, LHR, FSHR and IGF2 polymorphisms and scrotal circumference in Nellore bulls / Polimorfismos dos genes B4GALT1, LHR, FSHR e IGF2 e perímetro escrotal em touros da raça Nelore

We assessed whether single nucleotide polymorphisms (SNPs) in the genes beta 1,4- galactosyltransferase (B4GALT1), luteinizing hormone receptor (LHR), follicle-stimulating hormone receptor (FSHR) and insulin-like growth factor 2 (IGF2) could be molecular markers for scrotal circumference (SC) in Nellore bulls. Animals with positive (+, n = 104) and negative (-, n = 74) expected progeny difference for scrotal circumference at 365 days (EPD SC 365) were selected and their SNPs were analyzed by restriction fragment length polymorphism (RFLP). The correlation between EPD SC 365 and expected progeny difference for age at first birth (EPD AFB) was also investigated. The SNPs in B4GALT1 and FSHR was not different between two groups analyzed. The CC genotype for LHR gene was most frequent in animals with EPD SC 365(+), whereas the TT was most frequent in the EPD SC 365(-). For IGF2 the CT and CC were the most frequent genotypes observed in animals with positive and negative EPD SC 365, respectively. The EPD SC 365 was negatively correlated with the EPD AFB (r = 0.23). We suggest that CC and TT genotypes for LHR and IGF2, respectively, could be possible molecular markers for SC selection in Nellore bulls, that can also predict for AFB.(AU)

Foram avaliados se polimorfismos de base única (SNPs) presentes nos genes beta-1,4- galactosiltransferase (B4GALT1), receptor de hormônio luteinizante (LHR), receptor de hormônio folículo estimulante (FSHR) e fator de crescimento semelhante à insulina 2 (IGF2) poderiam ser marcadores moleculares para o perímetro escrotal (PE) em touros da raça Nelore. Animais com diferença esperada de progênie positiva (+, n = 104) e negativa (-, n = 74) para PE aos 365 dias (DEP PE 365) foram selecionados e seus SNPs foram analisados utilizando a técnica de polimorfismo de comprimento de fragmentos de restrição (RFLP). A correlação entre DEP PE 365 e idade ao primeiro parto (DEP IPP) também foi investigada. Os SNPs dos genes B4GALT1 e FSHR não apresentaram diferença entre os dois grupos analisados. O genótipo CC para o gene LHR foi mais freqüente em animais com DEP PE 365 (+), enquanto o TT foi mais frequente no grupo com DEP PE 365 (-). Para o gene IGF2, os genótipos CT e CC foram mais freqüentes em animais com DEP PE 365 positiva e negativa, respectivamente. A DEP PE 365 foi negativamente correlacionada com a DEP IPP (r = -0,23). O genótipo CC para o gene LHR e genótipo TT para o gene IGF2 podem ser possíveis marcadores de PE para a seleção assistida em touros da raça Nelore, podendo ser ainda preditores para IPP.(AU)

B4GALT1, LHR, FSHR and IGF2 polymorphisms and scrotal circumference in Nellore bulls / Polimorfismos dos genes B4GALT1, LHR, FSHR e IGF2 e perímetro escrotal em touros da raça Nelore

We assessed whether single nucleotide polymorphisms (SNPs) in the genes beta 1,4- galactosyltransferase (B4GALT1), luteinizing hormone receptor (LHR), follicle-stimulating hormone receptor (FSHR) and insulin-like growth factor 2 (IGF2) could be molecular markers for scrotal circumference (SC) in Nellore bulls. Animals with positive (+, n = 104) and negative (-, n = 74) expected progeny difference for scrotal circumference at 365 days (EPD SC 365) were selected and their SNPs were analyzed by restriction fragment length polymorphism (RFLP). The correlation between EPD SC 365 and expected progeny difference for age at first birth (EPD AFB) was also investigated. The SNPs in B4GALT1 and FSHR was not different between two groups analyzed. The CC genotype for LHR gene was most frequent in animals with EPD SC 365(+), whereas the TT was most frequent in the EPD SC 365(-). For IGF2 the CT and CC were the most frequent genotypes observed in animals with positive and negative EPD SC 365, respectively. The EPD SC 365 was negatively correlated with the EPD AFB (r = 0.23). We suggest that CC and TT genotypes for LHR and IGF2, respectively, could be possible molecular markers for SC selection in Nellore bulls, that can also predict for AFB.

Foram avaliados se polimorfismos de base única (SNPs) presentes nos genes beta-1,4- galactosiltransferase (B4GALT1), receptor de hormônio luteinizante (LHR), receptor de hormônio folículo estimulante (FSHR) e fator de crescimento semelhante à insulina 2 (IGF2) poderiam ser marcadores moleculares para o perímetro escrotal (PE) em touros da raça Nelore. Animais com diferença esperada de progênie positiva (+, n = 104) e negativa (-, n = 74) para PE aos 365 dias (DEP PE 365) foram selecionados e seus SNPs foram analisados utilizando a técnica de polimorfismo de comprimento de fragmentos de restrição (RFLP). A correlação entre DEP PE 365 e idade ao primeiro parto (DEP IPP) também foi investigada. Os SNPs dos genes B4GALT1 e FSHR não apresentaram diferença entre os dois grupos analisados. O genótipo CC para o gene LHR foi mais freqüente em animais com DEP PE 365 (+), enquanto o TT foi mais frequente no grupo com DEP PE 365 (-). Para o gene IGF2, os genótipos CT e CC foram mais freqüentes em animais com DEP PE 365 positiva e negativa, respectivamente. A DEP PE 365 foi negativamente correlacionada com a DEP IPP (r = -0,23). O genótipo CC para o gene LHR e genótipo TT para o gene IGF2 podem ser possíveis marcadores de PE para a seleção assistida em touros da raça Nelore, podendo ser ainda preditores para IPP.

Increased expression of ID2, PRELP and SMOC2 genes in patients with endometriosis.

Endometriosis is a benign, estrogen-dependent disease with symptoms such as pelvic pain and infertility, and it is characterized by the ectopic distribution of endometrial tissue. The expression of the ID2, PRELP and SMOC2 genes was compared between the endometrium of women without endometriosis in the proliferative phase of their menstrual cycle and the eutopic and ectopic endometrium of women with endometriosis in the proliferative phase. Paired tissue samples from 20 women were analyzed: 10 from endometrial and peritoneal endometriotic lesions and 10 from endometrial and ovarian endometriotic lesions. As controls, 16 endometrium samples were collected from women without endometriosis in the proliferative phase of menstrual cycle. Analysis was performed by real-time polymerase chain reaction (PCR). There was no significant difference between gene expression in the endometrium of women with and without endometriosis. The ID2 gene expression was increased in the most advanced stage of endometriosis and in ovarian endometriomas, the PRELP was more expressed in peritoneal lesions, and the SMOC2 was highly expressed in both peritoneal and endometrioma lesions. Considering that the genes studied participate either directly or indirectly in cellular processes that can lead to cell migration, angiogenesis, and inappropriate invasion, it is possible that the deregulation of these genes caused the development and maintenance of ectopic tissue.

Increased expression of ID2, PRELP and SMOC2 genes in patients with endometriosis

Endometriosis is a benign, estrogen-dependent disease with symptoms such as pelvic pain and infertility, and it is characterized by the ectopic distribution of endometrial tissue. The expression of the ID2, PRELP and SMOC2 genes was compared between the endometrium of women without endometriosis in the proliferative phase of their menstrual cycle and the eutopic and ectopic endometrium of women with endometriosis in the proliferative phase. Paired tissue samples from 20 women were analyzed: 10 from endometrial and peritoneal endometriotic lesions and 10 from endometrial and ovarian endometriotic lesions. As controls, 16 endometrium samples were collected from women without endometriosis in the proliferative phase of menstrual cycle. Analysis was performed by real-time polymerase chain reaction (PCR). There was no significant difference between gene expression in the endometrium of women with and without endometriosis. The ID2 gene expression was increased in the most advanced stage of endometriosis and in ovarian endometriomas, the PRELP was more expressed in peritoneal lesions, and the SMOC2 was highly expressed in both peritoneal and endometrioma lesions. Considering that the genes studied participate either directly or indirectly in cellular processes that can lead to cell migration, angiogenesis, and inappropriate invasion, it is possible that the deregulation of these genes caused the development and maintenance of ectopic tissue.

Strategies of rotational stocking management on weight gain and productivity of heifers grazing on marandu palisade grass / Estratégia de manejo em pastejo rotacionado no ganho de peso e produtividade de novilhas pastejando capim marandu

Pasture management can affect plant growth and interfere with the performance of the animals or the productivity of the production system. Marandu palisade grass swards subjected to year-round grazing by crossbred heifers were evaluated. Three stocking systems were tested: 1) a grazing cycle of 28 days (T28d); 2) when the sward reached a pre-grazing height of 30 cm (T0.3m), and 3) when the sward reached a pre-grazing height of 30 cm under irrigation (T-irrig0.3m). Heifers from treatments T28d and T0.3m were supplemented with dry matter at 1% of BW during winter. Statistical was a completely randomized block split-plot design. The light interception was higher (P<0.001) for T-irrig0.3m during winter. A lower leaf area index (P<0.001) was found for T28d, and T0.3m during winter and for T0.3m during autumn. There was an effect of stocking systems (P=0.0055), season (P<0.001) and interaction (P<0.0001) on weight gain (WG), stocking rate and productivity. The lowest WG was obtained in winter for the T-irrig0.3m treatment. The mean WG was 0.45, 0.49 and 0.43 kg/day for T28d, T0.3m and T-irrig0.3m, respectively. Lower productivity (P=0.0155) was observed for T28d when compared to T0.3m and T-irrig0.3m. The productivity rates were 662.2, 741.7 and 758.3 kg WG/ha, per season for T28d, T0.3m and T-irrig0.3m, respectively...(AU)

O manejo de pastagens pode afetar o crescimento das plantas e interferir no desempenho dos animais ou na produtividade do sistema de produção. Sistemas de pastejo de capim marandu foram avaliados durante um ano, sendo ocupados por novilhas mestiças. Três sistemas de pastejo foram avaliados: 1) ciclos de pastejos fixos com 28 dias (T28d); 2) pastejos iniciados quando a altura da planta era de 30 cm (T0,3m), e 3) pastejos iniciados quando a altura da planta era de 30 cm recebendo irrigação (T-irrig0,3m). As novilhas dos tratamentos T28d e T0,3m foram suplementadas durante o inverno seco em 1% do peso vivo. O delineamento foi em blocos casualisados e medidas repetidas no tempo. A interceptação luminosa foi maior (P<0,001) para T-irrig0,3m durante o inverno. Menor índice de área foliar (P<0,001) foi observada para T28d, eT0,3m no inverno e para T0,3m durante o outono. Houve efeito de sistema de pastejo (P=0,0055), estação do ano (P<0,001) e interação (P<0,0001) no ganho de peso (GP), taxa de lotação e produtividade. O menor GP foi obtido no inverno e no tratamento T-irrig0,3m. As médias de GP foram 0,45, 0,49 e 0,43 kg/dia para T28d, T0,3m e T-irrig0,3m, respectivamente. Menor produtividade (P=0,0155) foi observada em T28d quando comparado com T0,3m e T-irrig0,3m. As produtividades foram 662,2, 741,7 e 758,3 kg GP/ha por estação do ano para T28d, T0,3m e T-irrig0,3m, respectivamente...(AU)

Relação entre circunferência escrotal de touros Nelore e taxa de prenhez de vacas em monta natural / Relationship between scrotal circumference of nellore bulls and cow pregnancy rate in natural mating

A taxa de prenhez é de extrema importância para a eficiência produtiva de bovinos de corte, e a fertilidade dos touros pode influenciar na taxa de prenhez das matrizes em monta natural. O objetivo deste trabalho foi avaliar a relação entre as características do exame andrológico, especialmente a circunferência escrotal (CE), de touros da raça Nelore (Bos indicus), selecionados para reprodução com base em maior ganho de peso pós-desmama, com a taxa de prenhez de vacas Nelore em estação de monta natural de 90 dias de duração. Foram avaliados registros de 7171 entradas na monta de 1923 vacas submetidas a 216 touros em 368 lotes de estação de monta a campo (touro único), durante os anos de 1995 a 2014, e a CE dos touros. A CE foi pré-ajustada para idade do touro (2,67±0,62 anos) na entrada da estação de monta, em modelo de regressão linear, sendo posteriormente expressa em 3 classes: classe 1: CE < 35 cm; classe 2: 35 ≥ CE < 37 cm; classe 3: CE ≥ 37. Mais da metade das observações de CE ficaram na classe 2, e 74,52% das exposições aos touros resultaram em prenhez, com mínimo de 0% e máximo de 100% de sucesso de prenhez nos 368 lotes de monta. Parte (n=33) dos 216 touros tinham registros de características obtidas em exame andrológico parcial [CE, volume, motilidade, vigor, concentração e morfologia espermática (defeitos maiores e menores)]. Verificou-se que a classe de valores intermediários de CE (classe 2: 35 ≥ CE < 37 cm) foi a de maior risco relativo para sucesso na prenhez. Na amostra dos 33 touros, a taxa de prenhez apresentou correlação moderada e positiva (r = 0,66) somente com a concentração espermática. A fraca relação entre as características do andrológico os touros e taxa de prenhez das vacas pode ser devido ao fato da amostra dos touros avaliados neste tipo de trabalho ser sempre viesada. Ou seja, só são considerados os touros que entraram em monta...(AU)

The pregnancy rate is extremely important for the productive efficiency of beef cattle and bull fertility can influence the pregnancy rate of cows in natural mating. The objective of this study was to evaluate the relationship of andrological traits, especially scrotal circumference (SC), of Nellore bulls (Bos indicus) selected for breeding based on greater postweaning weight gain with the pregnancy rate of naturally mated Nellore cows in a 90-day breeding season. Records of 7,171 entries into the breeding season of 1,923 cows exposed to 216 bulls in 368 mating batches in the field (single bull) from 1995 to 2014, as well as records of SC, were evaluated. Scrotal circumference was pre-adjusted for bull age (2.67±0.62 years) at the start of the breeding season using a linear regression model and was divided into three classes: class 1: SC < 35 cm; class 2: 35 ≥ SC < 37 cm; class 3: SC ≥ 37. More than half the observations of SC were in class 2 and 74.52% of the exposures to bulls resulted in pregnancy, with a minimum rate of successful pregnancy of 0% and a maximum rate of 100% in the 368 mating batches. Thirty-three of the 216 bulls had records of andrological traits [SC, sperm volume, motility, vigor, concentration and morphology (major and minor defects)]. The class of intermediate SC values (class 2: 35 ≥ SC < 37 cm) was associated with a higher relative risk of pregnancy success. In the sample of the 33 bulls, pregnancy rate showed a moderate positive correlation (r = 0.66) only with sperm concentration. The weak association between andrological traits of bulls and pregnancy rate might be due to the fact that the sample of bulls evaluated in this type of study will be always biased. In other words, only bulls that enter the breeding season are considered, which correspond to a small percentage of all males born. Consequently, the variation observed in these bulls does...(AU)

Strategies of rotational stocking management on weight gain and productivity of heifers grazing on marandu palisade grass / Estratégia de manejo em pastejo rotacionado no ganho de peso e produtividade de novilhas pastejando capim marandu

Pasture management can affect plant growth and interfere with the performance of the animals or the productivity of the production system. Marandu palisade grass swards subjected to year-round grazing by crossbred heifers were evaluated. Three stocking systems were tested: 1) a grazing cycle of 28 days (T28d); 2) when the sward reached a pre-grazing height of 30 cm (T0.3m), and 3) when the sward reached a pre-grazing height of 30 cm under irrigation (T-irrig0.3m). Heifers from treatments T28d and T0.3m were supplemented with dry matter at 1% of BW during winter. Statistical was a completely randomized block split-plot design. The light interception was higher (P<0.001) for T-irrig0.3m during winter. A lower leaf area index (P<0.001) was found for T28d, and T0.3m during winter and for T0.3m during autumn. There was an effect of stocking systems (P=0.0055), season (P<0.001) and interaction (P<0.0001) on weight gain (WG), stocking rate and productivity. The lowest WG was obtained in winter for the T-irrig0.3m treatment. The mean WG was 0.45, 0.49 and 0.43 kg/day for T28d, T0.3m and T-irrig0.3m, respectively. Lower productivity (P=0.0155) was observed for T28d when compared to T0.3m and T-irrig0.3m. The productivity rates were 662.2, 741.7 and 758.3 kg WG/ha, per season for T28d, T0.3m and T-irrig0.3m, respectively...

O manejo de pastagens pode afetar o crescimento das plantas e interferir no desempenho dos animais ou na produtividade do sistema de produção. Sistemas de pastejo de capim marandu foram avaliados durante um ano, sendo ocupados por novilhas mestiças. Três sistemas de pastejo foram avaliados: 1) ciclos de pastejos fixos com 28 dias (T28d); 2) pastejos iniciados quando a altura da planta era de 30 cm (T0,3m), e 3) pastejos iniciados quando a altura da planta era de 30 cm recebendo irrigação (T-irrig0,3m). As novilhas dos tratamentos T28d e T0,3m foram suplementadas durante o inverno seco em 1% do peso vivo. O delineamento foi em blocos casualisados e medidas repetidas no tempo. A interceptação luminosa foi maior (P<0,001) para T-irrig0,3m durante o inverno. Menor índice de área foliar (P<0,001) foi observada para T28d, eT0,3m no inverno e para T0,3m durante o outono. Houve efeito de sistema de pastejo (P=0,0055), estação do ano (P<0,001) e interação (P<0,0001) no ganho de peso (GP), taxa de lotação e produtividade. O menor GP foi obtido no inverno e no tratamento T-irrig0,3m. As médias de GP foram 0,45, 0,49 e 0,43 kg/dia para T28d, T0,3m e T-irrig0,3m, respectivamente. Menor produtividade (P=0,0155) foi observada em T28d quando comparado com T0,3m e T-irrig0,3m. As produtividades foram 662,2, 741,7 e 758,3 kg GP/ha por estação do ano para T28d, T0,3m e T-irrig0,3m, respectivamente...

Relação entre circunferência escrotal de touros Nelore e taxa de prenhez de vacas em monta natural / Relationship between scrotal circumference of nellore bulls and cow pregnancy rate in natural mating

A taxa de prenhez é de extrema importância para a eficiência produtiva de bovinos de corte, e a fertilidade dos touros pode influenciar na taxa de prenhez das matrizes em monta natural. O objetivo deste trabalho foi avaliar a relação entre as características do exame andrológico, especialmente a circunferência escrotal (CE), de touros da raça Nelore (Bos indicus), selecionados para reprodução com base em maior ganho de peso pós-desmama, com a taxa de prenhez de vacas Nelore em estação de monta natural de 90 dias de duração. Foram avaliados registros de 7171 entradas na monta de 1923 vacas submetidas a 216 touros em 368 lotes de estação de monta a campo (touro único), durante os anos de 1995 a 2014, e a CE dos touros. A CE foi pré-ajustada para idade do touro (2,67±0,62 anos) na entrada da estação de monta, em modelo de regressão linear, sendo posteriormente expressa em 3 classes: classe 1: CE < 35 cm; classe 2: 35 ≥ CE < 37 cm; classe 3: CE ≥ 37. Mais da metade das observações de CE ficaram na classe 2, e 74,52% das exposições aos touros resultaram em prenhez, com mínimo de 0% e máximo de 100% de sucesso de prenhez nos 368 lotes de monta. Parte (n=33) dos 216 touros tinham registros de características obtidas em exame andrológico parcial [CE, volume, motilidade, vigor, concentração e morfologia espermática (defeitos maiores e menores)]. Verificou-se que a classe de valores intermediários de CE (classe 2: 35 ≥ CE < 37 cm) foi a de maior risco relativo para sucesso na prenhez. Na amostra dos 33 touros, a taxa de prenhez apresentou correlação moderada e positiva (r = 0,66) somente com a concentração espermática. A fraca relação entre as características do andrológico os touros e taxa de prenhez das vacas pode ser devido ao fato da amostra dos touros avaliados neste tipo de trabalho ser sempre viesada. Ou seja, só são considerados os touros que entraram em monta...

The pregnancy rate is extremely important for the productive efficiency of beef cattle and bull fertility can influence the pregnancy rate of cows in natural mating. The objective of this study was to evaluate the relationship of andrological traits, especially scrotal circumference (SC), of Nellore bulls (Bos indicus) selected for breeding based on greater postweaning weight gain with the pregnancy rate of naturally mated Nellore cows in a 90-day breeding season. Records of 7,171 entries into the breeding season of 1,923 cows exposed to 216 bulls in 368 mating batches in the field (single bull) from 1995 to 2014, as well as records of SC, were evaluated. Scrotal circumference was pre-adjusted for bull age (2.67±0.62 years) at the start of the breeding season using a linear regression model and was divided into three classes: class 1: SC < 35 cm; class 2: 35 ≥ SC < 37 cm; class 3: SC ≥ 37. More than half the observations of SC were in class 2 and 74.52% of the exposures to bulls resulted in pregnancy, with a minimum rate of successful pregnancy of 0% and a maximum rate of 100% in the 368 mating batches. Thirty-three of the 216 bulls had records of andrological traits [SC, sperm volume, motility, vigor, concentration and morphology (major and minor defects)]. The class of intermediate SC values (class 2: 35 ≥ SC < 37 cm) was associated with a higher relative risk of pregnancy success. In the sample of the 33 bulls, pregnancy rate showed a moderate positive correlation (r = 0.66) only with sperm concentration. The weak association between andrological traits of bulls and pregnancy rate might be due to the fact that the sample of bulls evaluated in this type of study will be always biased. In other words, only bulls that enter the breeding season are considered, which correspond to a small percentage of all males born. Consequently, the variation observed in these bulls does...

Growth curves of carcass traits obtained by ultrasonography in three lines of Nellore cattle selected for body weight.

The effect of selection for postweaning weight was evaluated within the growth curve parameters for both growth and carcass traits. Records of 2404 Nellore animals from three selection lines were analyzed: two selection lines for high postweaning weight, selection (NeS) and traditional (NeT); and a control line (NeC) in which animals were selected for postweaning weight close to the average. Body weight (BW), hip height (HH), rib eye area (REA), back fat thickness (BFT), and rump fat thickness (RFT) were measured and records collected from animals 8 to 20 (males) and 11 to 26 (females) months of age. The parameters A (asymptotic value) and k (growth rate) were estimated using the nonlinear model procedure of the Statistical Analysis System program, which included fixed effect of line (NeS, NeT, and NeC) in the model, with the objective to evaluate differences in the estimated parameters between lines. Selected animals (NeS and NeT) showed higher growth rates than control line animals (NeC) for all traits. Line effect on curves parameters was significant (P < 0.001) for BW, HH, and REA in males, and for BFT and RFT in females. Selection for postweaning weight was effective in altering growth curves, resulting in animals with higher growth potential.

Parâmetros genéticos para período de gestação e características de crescimento pré e pós desmame em bovinos Nelore / Genetic parameters for gestation length and pre- and postweaning growth traits in Nellore cattle

O objetivo deste estudo foi estimar as herdabilidades e as correlações genéticas e fenotípicas para o período de gestação como característica do bezerro (PGbez) e características de desempenho pré desmame: peso ao nascimento (PN), peso ao 120 dias (P120) e peso ao 210 dias (P210), e características de desempenho pós-desmame: peso aos 365 dias e peso aos 450 dias (P450) em animais da raça Nelore participantes do Programa Nelore Brasil coordenado pela Associação Nacional de Criadores e Pesquisadores (ANCP). Os parâmetros foram estimados utilizando o método de máxima verossimilhança restrita por modelo animal, usando o software WOMBAT. A correlação genética entre PGbez e PN foi 0,15, indicando associação favorável entre as duas características, porém de pequena magnitude. O PGbez apresentou correlações genéticas positivas e de baixa magnitude (0,06 a 0,10) com os demais pesos, indicando que a seleção para a diminuição do período de gestação não acarretaria em perdas significativas nos pesos em idades mais avançadas. Além disso, é possível inferir que a seleção para maior P365 não aumentará expressivamente o período de gestação...(AU)

The objective of this study was to estimate heritabilities and genetic and phenotypic correlations for gestation length as calf trait (GLcalf) and preweaning [birth weight (BW) and weights at 120 (W120) and 210 (W210) days] and postweaning performance traits [weights at 365 (W365) and 450 (W450) days] in Nellore cattle participating in the Nellore Brazil Program coordinated by the National Association of Breeders and Researchers (Associação Nacional de Criadores e Pesquisadores - ANCP). The parameters were estimated by the restricted maximum likelihood method under an animal model using the WOMBAT software. The genetic correlation between GLcalf and BW was 0.15, indicating a favorable association between the two traits which, however, was of small magnitude. GLcalf showed low positive genetic correlations (0.06 to 0.10) with the other weights, indicating that selection to reduce gestation length would not result in significant losses in weights at older ages. Furthermore, it is possible to infer that selection for higher W365 will not expressively increase gestation length...(AU)

Parâmetros genéticos para período de gestação e características de crescimento pré e pós desmame em bovinos Nelore / Genetic parameters for gestation length and pre- and postweaning growth traits in Nellore cattle

O objetivo deste estudo foi estimar as herdabilidades e as correlações genéticas e fenotípicas para o período de gestação como característica do bezerro (PGbez) e características de desempenho pré desmame: peso ao nascimento (PN), peso ao 120 dias (P120) e peso ao 210 dias (P210), e características de desempenho pós-desmame: peso aos 365 dias e peso aos 450 dias (P450) em animais da raça Nelore participantes do Programa Nelore Brasil coordenado pela Associação Nacional de Criadores e Pesquisadores (ANCP). Os parâmetros foram estimados utilizando o método de máxima verossimilhança restrita por modelo animal, usando o software WOMBAT. A correlação genética entre PGbez e PN foi 0,15, indicando associação favorável entre as duas características, porém de pequena magnitude. O PGbez apresentou correlações genéticas positivas e de baixa magnitude (0,06 a 0,10) com os demais pesos, indicando que a seleção para a diminuição do período de gestação não acarretaria em perdas significativas nos pesos em idades mais avançadas. Além disso, é possível inferir que a seleção para maior P365 não aumentará expressivamente o período de gestação...

The objective of this study was to estimate heritabilities and genetic and phenotypic correlations for gestation length as calf trait (GLcalf) and preweaning [birth weight (BW) and weights at 120 (W120) and 210 (W210) days] and postweaning performance traits [weights at 365 (W365) and 450 (W450) days] in Nellore cattle participating in the Nellore Brazil Program coordinated by the National Association of Breeders and Researchers (Associação Nacional de Criadores e Pesquisadores - ANCP). The parameters were estimated by the restricted maximum likelihood method under an animal model using the WOMBAT software. The genetic correlation between GLcalf and BW was 0.15, indicating a favorable association between the two traits which, however, was of small magnitude. GLcalf showed low positive genetic correlations (0.06 to 0.10) with the other weights, indicating that selection to reduce gestation length would not result in significant losses in weights at older ages. Furthermore, it is possible to infer that selection for higher W365 will not expressively increase gestation length...

Follicular fluid from infertile women with mild endometriosis may compromise the meiotic spindles of bovine metaphase II oocytes.

STUDY QUESTION: What is the potential impact of follicular fluid (FF) from infertile women with mild endometriosis (ME) on oocyte quality, especially on nuclear maturation and the meiotic spindle? SUMMARY ANSWER: FF from infertile women with ME may compromise nuclear maturation and the meiotic spindles of in vitro matured bovine oocytes. WHAT IS KNOWN ALREADY: Controversial studies have suggested that impaired oocyte quality may be involved in the pathogenesis of endometriosis-related infertility. Moreover, some studies have demonstrated alterations in the composition of FF from infertile women with endometriosis. However, to date no study has evaluated the effect of FF from infertile women with ME on the genesis of meiotic oocyte anomalies. STUDY DESIGN, SIZE, DURATION: We performed an experimental study. Samples of FF were obtained from February 2009 to February 2011 from 22 infertile women, 11 with ME and 11 with tubal or male factors of infertility (control group), who underwent ovarian stimulation for ICSI at our university IVF Unit. From March 2011 to February 2012 we performed in vitro maturation (IVM) experiments using immature bovine oocytes as described below. PARTICIPANTS/MATERIALS, SETTING, METHODS: FF free of blood and containing a mature oocyte was obtained from 22 infertile women during oocyte retrieval for ICSI. Immature bovine oocytes underwent IVM in the absence of FF (No-FF) and in the presence of four concentrations (1, 5, 10 and 15%) of FF from infertile women without endometriosis (C-FF) and with ME (ME-FF). Eleven replicates were performed, each one using FF from a control patient and a patient with ME. Each FF sample was used in only one experiment. After 22-24 h of IVM, oocytes were denuded, fixed and immunostained for morphological visualization of microtubules and chromatin by confocal microscopy. MAIN RESULTS AND THE ROLE OF CHANCE: A total of 1324 cumulus-oocyte complexes were matured in vitro. Of these, 1128 were fixed and 1048 were analyzed by confocal microscopy. The percentage of meiotically normal oocytes was significantly higher for oocytes that underwent IVM in the absence of FF (No-FF; 76.5%) and in the presence of 1% (80.9%), 5% (76.6%), 10% (75%) and 15% (76.2%) C-FF than in oocytes that underwent IVM in the presence of 1% (44.4%), 5% (36.7%), 10% (45.5%) and 15% (51.2%) ME-FF (P < 0.01). No differences were observed among FF concentrations within each group. When the four concentrations from each group were pooled, the number of oocytes in metaphase I stage was significantly higher in the ME-FF (50 oocytes) than in the C-FF (29 oocytes) group and the percentage of meiotic abnormalities was significantly higher when oocytes were matured with ME-FF (55.8%) than with C-FF (23.1%), P < 0.01. LIMITATIONS, REASONS FOR CAUTION: Owing to the strict selection criteria for FF donors, this study had a small sample size (11 cases and 11 controls), and thus further investigations using a large cohort of patients are needed to confirm these results. In addition, data obtained from studies using animal models may not necessarily be extrapolated to humans and studies evaluating in vivo matured oocytes from infertile women with ME are important to confirm our results. WIDER IMPLICATIONS OF THE FINDINGS: Our results open new insights into the pathogenic mechanisms of infertility related to mild endometriosis, suggesting that FF from infertile women with mild endometriosis may be involved in the worsening of oocyte quality of these women. STUDY FUNDING/COMPETING INTEREST(S): This study was supported by the National Council for Scientific and Technological Development (CNPq), Brazil. The authors declare no conflicts of interest.

Placental hydroxymethylation vs methylation at the imprinting control region 2 on chromosome 11p15.5.

In addition to methylated cytosines (5-mCs), hydroxymethylcytosines (5-hmCs) are present in CpG dinucleotide-enriched regions and some transcription regulator binding sites. Unlike methylation, hydroxymethylation does not result in silencing of gene expression, and the most commonly used methods to study methylation, such as techniques based on restriction enzymatic digestion and/or bisulfite modification, are unable to distinguish between them. Genomic imprinting is a process of gene regulation where only one member of an allelic pair is expressed depending on the parental origin. Chromosome 11p15.5 has an imprinting control region (ICR2) that includes a differentially methylated region (KvDMR1) that guarantees parent-specific gene expression. The objective of the present study was to determine the presence of 5-hmC at the KvDMR1 in human placentas. We analyzed 16 third-trimester normal human placentas (chorionic villi). We compared two different methods based on real-time PCR after enzymatic digestion. The first method distinguished methylation from hydroxymethylation, while the other method did not. Unlike other methylation studies, subtle variations of methylation in ICRs could represent a drastic deregulation of the expression of imprinted genes, leading to important phenotypic consequences, and the presence of hydroxymethylation could interfere with the results of many studies. We observed agreement between the results of both methods, indicating the absence of hydroxymethylation at the KvDMR1 in third-trimester placentas. To the best of our knowledge, this is the first study describing the investigation of hydroxymethylation in human placenta using a genomic imprinting model.

Placental hydroxymethylation vs methylation at the imprinting control region 2 on chromosome 11p15.5

In addition to methylated cytosines (5-mCs), hydroxymethylcytosines (5-hmCs) are present in CpG dinucleotide-enriched regions and some transcription regulator binding sites. Unlike methylation, hydroxymethylation does not result in silencing of gene expression, and the most commonly used methods to study methylation, such as techniques based on restriction enzymatic digestion and/or bisulfite modification, are unable to distinguish between them. Genomic imprinting is a process of gene regulation where only one member of an allelic pair is expressed depending on the parental origin. Chromosome 11p15.5 has an imprinting control region (ICR2) that includes a differentially methylated region (KvDMR1) that guarantees parent-specific gene expression. The objective of the present study was to determine the presence of 5-hmC at the KvDMR1 in human placentas. We analyzed 16 third-trimester normal human placentas (chorionic villi). We compared two different methods based on real-time PCR after enzymatic digestion. The first method distinguished methylation from hydroxymethylation, while the other method did not. Unlike other methylation studies, subtle variations of methylation in ICRs could represent a drastic deregulation of the expression of imprinted genes, leading to important phenotypic consequences, and the presence of hydroxymethylation could interfere with the results of many studies. We observed agreement between the results of both methods, indicating the absence of hydroxymethylation at the KvDMR1 in third-trimester placentas. To the best of our knowledge, this is the first study describing the investigation of hydroxymethylation in human placenta using a genomic imprinting model.

Characterization of the variable cow's age at last calving as a measurement of longevity by using the Kaplan-Meier estimator and the Cox model.

In most studies on beef cattle longevity, only the cows reaching a given number of calvings by a specific age are considered in the analyses. With the aim of evaluating all cows with productive life in herds, taking into consideration the different forms of management on each farm, it was proposed to measure cow longevity from age at last calving (ALC), that is, the most recent calving registered in the files. The objective was to characterize this trait in order to study the longevity of Nellore cattle, using the Kaplan-Meier estimators and the Cox model. The covariables and class effects considered in the models were age at first calving (AFC), year and season of birth of the cow and farm. The variable studied (ALC) was classified as presenting complete information (uncensored = 1) or incomplete information (censored = 0), using the criterion of the difference between the date of each cow's last calving and the date of the latest calving at each farm. If this difference was >36 months, the cow was considered to have failed. If not, this cow was censored, thus indicating that future calving remained possible for this cow. The records of 11 791 animals from 22 farms within the Nellore Breed Genetic Improvement Program ('Nellore Brazil') were used. In the estimation process using the Kaplan-Meier model, the variable of AFC was classified into three age groups. In individual analyses, the log-rank test and the Wilcoxon test in the Kaplan-Meier model showed that all covariables and class effects had significant effects (P < 0.05) on ALC. In the analysis considering all covariables and class effects, using the Wald test in the Cox model, only the season of birth of the cow was not significant for ALC (P > 0.05). This analysis indicated that each month added to AFC diminished the risk of the cow's failure in the herd by 2%. Nonetheless, this does not imply that animals with younger AFC had less profitability. Cows with greater numbers of calvings were more precocious than those with fewer calvings.

Genetic analysis on growth and carcass traits in Nelore cattle.

In this study, the objective was to estimate genetic parameters of body weight at 210 (BW210) and 365 (BW365) days of age in relation to rib eye area (REA), subcutaneous back fat thickness (BF) and rump fat (RF), and their respective genetic trends, in Nelore beef cattle. Estimates of genetic parameters and breeding values for the studied traits were obtained using the REML method. The direct and maternal heritability estimates were respectively: 0.25±0.02 and 0.21±0.01, for BW210, and 0.29±0.02 and 0.09±0.01, for BW365. The heritability estimates for transformed REA, BF and RF were 0.29±0.03, 0.21±0.02 and 0.23±0.03, respectively. There were genetic associations between BW210 and REA, BW365 and REA, and BF and RF, while the other correlations were low. The selection process that was conducted at the farms participating in the breeding program, taking the proposed selection index into consideration, caused genetic changes to these traits.

Genetic associations between age at first calving and heifer body weight and scrotal circumference in Nelore cattle.

Summary Age at first calving (AFC) measures the entry of heifers into the beef cattle production system. This trait can be used as a selection criterion for earlier reproductive performance. Using data from Nelore cattle participating in the 'Program for Genetic Improvement of the Nelore Breed' (PMGRN-Nelore Brazil), bi-trait analyses were performed using the restricted maximum likelihood method, based on an AFC animal model and the following traits: female body weight adjusted to 365 (BW365) and 450 (BW450) days of age, and male scrotal circumference adjusted to 365 (SC365), 450 (SC450), 550 (SC550) and 730 (SC730) days of age. The heritability estimates for AFC ranged from 0.02 +/- 0.02 to 0.04 +/- 0.02. The estimates of additive direct heritabilities (with standard error) for BW365, BW450, SC365, SC450, SC550 and SC730 were 0.36 +/- 0.07, 0.38 +/- 0.07, 0.48 +/- 0.07, 0.65 +/- 0.07, 0.64 +/- 0.07 and 0.42 +/- 0.07, respectively, and the genetic correlations with AFC were -0.38, -0.33, 0.10, -0.13, -0.13 and 0.06, respectively. In the herds studied, selection for SC365, SC450, SC550 or SC730 should not cause genetic changes in AFC. Selection based on BW365 or BW450 would favor smaller AFC breeding values. However, the low magnitude of direct heritability estimates for AFC in these farms indicates that changes in phenotypical expression depend mostly on non-genetic factors.