RESUMO
The effects of retinoic acid (RA) on cell proliferation, activity of acid phosphatase, protein synthesis and methionine uptake were studied in transformed murine LPA cells. Early inhibition of protein synthesis was demonstrated under experimental conditions in which the rate of cell proliferation was diminished and non-specific effects of vitamin action could be excluded. Measurements of L-methionine uptake revealed a decrease to approximately one-half of that in control cultures after treatment with RA at the concentrations of 5 X 10(-5) M and 10(-4) M.
Assuntos
Metionina/metabolismo , Biossíntese de Proteínas , Tretinoína/farmacologia , Fosfatase Ácida/metabolismo , Animais , Transporte Biológico/efeitos dos fármacos , Divisão Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Transformação Celular Neoplásica , Relação Dose-Resposta a Droga , Cinética , Células L , CamundongosRESUMO
The behaviour of subcultures of vitamin A-treated human embryo fibroblasts was studied from the point of view of morphology, growth characteristics, karyology and agglutinability at subsequent passages after treatment. Evidence was obtained that vitamin A-induced rupture of lysosomes may lead to transient alterations in the pattern of growth, structure of the membranes and morphology in cell generations deriving from the cells injured by the action of the vitamin.
