RESUMO
A methanolic extract of Commelina communis showed potent inhibitory activity against alpha-glucosidase. One pyrrolidine alkaloid, 2,5-dihydroxymethyl-3,4-dihydroxypyrrolidine (DMDP, 1) and four piperidine alkaloids, 1-deoxymannojirimycin (2), 1-deoxynojirimycin (3), alpha-homonojirimycin (4) and 7-O-beta-D-glucopyranosyl alpha-homonojirimycin (5) were isolated by bioassay-directed fractionation and separation. These compounds have been identified for the first time from Commelina communis, supporting the pharmacological basis of this plant that has been used as a traditional herbal medicine for the treatment of diabetes.
Assuntos
Alcaloides/isolamento & purificação , Inibidores Enzimáticos/isolamento & purificação , Inibidores de Glicosídeo Hidrolases , Plantas Medicinais , Alcaloides/química , Alcaloides/farmacologia , Animais , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Nozes/enzimologia , Oryza/enzimologia , Pâncreas/enzimologia , Rhizopus/enzimologia , Saccharomyces cerevisiae/enzimologia , SuínosRESUMO
A transgalactosylation reaction from p-nitrophenyl-α-D-galactopyranoside to 1-deoxynojirimycin was done using α-galactosidase [EC 3.2.1.22] from green coffee beans. The enzyme formed 6-O-α-D-galactopyranosyl-1-deoxynojirimycin as the major product.
