Endogenous Enzymatic Activity of Primary and Permanent Dentine.

Matrix metalloproteinases (MMPs) play an important role in tooth development and influence caries development and hybrid layer degradation. Literature is scant on the differences in the activity of MMPs between primary and permanent dentine. Accordingly, the aim of the present study was to investigate endogenous gelatinolytic activity in primary and permanent dentine. Separate batches of dentine powder were obtained from intact human primary and permanent molars (n = 6). Each batch was divided in two subgroups: (1) mineralised; and (2) demineralised with 10% H3PO4. After protein extraction, gelatine zymography was performed. Furthermore, in situ zymography was performed on dentine sections of the same groups (n = 3). The slices were polished, covered with fluorescein-conjugated gelatine and evaluated using a confocal microscope. In situ zymography data were analysed using two-way analysis of variance and post hoc Holm-Sidák statistics (α = 0.05). Primary dentine showed poorly defined bands in the zymograms that vaguely corresponded to the pro-form and active form of MMP-2 and the pro-form of MMP-9. In permanent dentine, demineralised powder demonstrated stronger gelatinolytic activity than mineralised powder. In situ zymography identified stronger enzymatic activity in primary etched dentine (p < 0.05). Stronger enzymatic activity recorded in primary dentine may be related to the differences in morphology and composition between primary and permanent dentine.

Using gamification and IoT-based educational tools towards energy savings - some experiences from two schools in Italy and Greece.

Climate change and the need for sustainable development have become part of our daily lives. In this context, it is crucial to involve the educational community to the discussion, both students and teachers; by increasing awareness about these issues and the ways school communities can contribute to energy savings, we can kick-start a change towards more sustainable practices in our societies. The Green Awareness in Action (GAIA) H2020 research project implemented an IoT-based approach in several European schools for sustainability awareness and energy efficiency, while at the same time aiming for increasing students' digital skills. By using gamification, competitions and IoT-based educational activities, GAIA engaged directly with teachers and students in order to realize energy-saving activities in their environment. We report here on the use of gamification and competition among schools in this context, and how they helped together with IoT-based lab activities to engage students and educators to participate in the project more actively. We provide details on the implementation of GAIA's intervention in specific school settings to showcase our approach. Our findings, backed up by evaluation data and answers to a survey by 30 educators in Greece and Italy, confirm that the inclusion of competition and gamification aspects can significantly increase students' engagement, especially when having groups/schools competing with each other. Moreover, IoT-based educational activities can supplement existing educational activities in interesting ways, with students evaluating positively the experience and educators reporting increased overall student engagement in their class during the intervention period, and, on average, better class performance compared to previous periods.

Effect of Chitosan as a Cross-Linker on Matrix Metalloproteinase Activity and Bond Stability with Different Adhesive Systems.

The aim of the present study was to evaluate the effect of 0.1% chitosan (Ch) solution as an additional primer on the mechanical durability and enzymatic activity on dentine using an etch-and-rinse (E&R) adhesive and a universal self-etch (SE) adhesive. Microtensile bond strength and interfacial nanoleakage expression of the bonded interfaces for all adhesives (with or without pretreatment with 0.1% Ch solution for 1 min and air-dried for 5 seconds) were analyzed immediately and after 10,000 thermocycles. Zymograms of protein extracts from human dentine powder incubated with Optibond FL and Scotchbond Universal on untreated or Ch-treated dentine were obtained to examine dentine matrix metalloproteinase (MMP) activities. The use of 0.1% Ch solution as an additional primer in conjunction with the E&R or SE adhesive did not appear to have influenced the immediate bond strength (T0) or bond strength after thermocycling (T1). Zymography showed a reduction in MMP activities only for mineralized and demineralized dentine powder after the application of Ch. Application of 0.1% Ch solution does not increase the longevity of resin-dentine bonds. Nonetheless, the procedure appears to be proficient in reducing dentine MMP activities within groups without adhesive treatments. Further studies are required to comprehend the cross-linking of Ch with dentine collagen.

Design of a RESTful web information system for drug prescription and administration.

Drug prescription and administration processes strongly impact on the occurrence of risks in medical settings for they can be sources of adverse drug events (ADEs). A properly engineered use of information and communication technologies has proven to be a promising approach to reduce these risks. In this study, we propose PHARMA, a web information system which supports healthcare staff in the secure cooperative execution of drug prescription, transcription and registration tasks. PHARMA allows the easy sharing and management of documents containing drug-related information (i.e., drug prescriptions, medical reports, screening), which is often inconsistent and scattered across different information systems and heterogeneous organization domains (e.g., departments, other hospital facilities). PHARMA enables users to access such information in a consistent and secure way, through the adoption of REST and web-oriented design paradigms and protocols. We describe the implementation of the PHARMA prototype, and we discuss the results of the usability evaluation that we carried out with the staff of a hospital in Florence, Italy.

An ontology-based system for context-aware and configurable services to support home-based continuous care.

Continuous care models for chronic diseases pose several technology-oriented challenges for home-based care, where assistance services rely on a close collaboration among different stakeholders, such as health operators, patient relatives, and social community members. This paper describes an ontology-based context model and a related context management system providing a configurable and extensible service-oriented framework to ease the development of applications for monitoring and handling patient chronic conditions. The system has been developed in a prototypal version, and integrated with a service platform for supporting operators of home-based care networks in cooperating and sharing patient-related information and coordinating mutual interventions for handling critical and alarm situations. Finally, we discuss experimentation results and possible further research directions.

ERMHAN: A Context-Aware Service Platform to Support Continuous Care Networks for Home-Based Assistance.

Continuous care models for chronic diseases pose several technology-oriented challenges for home-based continuous care, where assistance services rely on a close collaboration among different stakeholders such as health operators, patient relatives, and social community members. Here we describe Emilia Romagna Mobile Health Assistance Network (ERMHAN) a multichannel context-aware service platform designed to support care networks in cooperating and sharing information with the goal of improving patient quality of life. In order to meet extensibility and flexibility requirements, this platform has been developed through ontology-based context-aware computing and a service oriented approach. We also provide some preliminary results of performance analysis and user survey activity.

Grammatical gender effects on cognition: implications for language learning and language use.

In 4 experiments, the authors addressed the mechanisms by which grammatical gender (in Italian and German) may come to affect meaning. In Experiments 1 (similarity judgments) and 2 (semantic substitution errors), the authors found Italian gender effects for animals but not for artifacts; Experiment 3 revealed no comparable effects in German. These results suggest that gender effects arise as a generalization from an established association between gender of nouns and sex of human referents, extending to nouns referring to sexuated entities. Across languages, such effects are found when the language allows for easy mapping between gender of nouns and sex of human referents (Italian) but not when the mapping is less transparent (German). A final experiment provided further constraints: These effects during processing arise at a lexical-semantic level rather than at a conceptual level.

Peroxidation potential of lipid emulsions after compounding in all-in-one solutions.

OBJECTIVES: We investigated the peroxidation potential of fat emulsions in all-in-one solutions (AIOs). METHODS: Three 20% emulsions were compared: soybean oil (SO; 60% polyunsaturated fatty acids [PUFAs], alpha-tocopherol:PUFAs = 0.44), soybean plus medium-chain triacylglycerol (SO-MCT; 31% PUFAs, alpha-tocopherol:PUFAs = 0.35), and olive oil (OO; 21% PUFAs, alpha-tocopherol:PUFAs = 1.42). For each emulsion, six AIO solutions were prepared by adding 250 mL of emulsion to a lipid-free solution. Lipid peroxide (LPX) and malondialdehyde (MDA) concentrations were evaluated in fat emulsions, lipid-free solutions, and AIOs immediately (T0) and 24 h (T24) after lipid addition. Statistical analysis was done with analysis of variance. RESULTS: Fat emulsion LPX in SO-MCT was lower than that in SO (P = 0.015) and OO (P = 0.024); LPX in SO was greater than that in OO (P = 0.013); MDA in SO was greater than that in SO-MCT (P = 0.001) and OO (P = 0.013); and MDA in SO-MCT was greater than that in OO (P = 0.001). In comparison with MDA at AIO-T0, MDA at AIO-T24 increased in SO (P = 0.005) and SO-MCT (P < 0.001) and decreased in OO (P = 0.003); at AIO-T24, LPX was greater in SO, but not significantly. CONCLUSIONS: In AIO bags, LPX occurred within 24 h after the addition of the lipid emulsion and seemed to be directly related to the PUFA content and inversely related to the alpha-tocopherol:PUFA ratio of the emulsion.

An investigation of semantic errors in unimpaired and Alzheimer's speakers of Italian.

Semantic errors are a common type of slip of the tongue for normal speakers; they are also considered to be the hallmark of progressive diseases that affect semantic memory such as Alzheimer's Disease (AD) and semantic dementia. In unimpaired speakers, semantic errors have been shown to be affected by syntactic variables. For example, Marx (1999) has shown that speakers of a gendered language such as German tend to substitute a target with another word that shares the same grammatical gender with the target more often than chance would predict. This finding suggests that errors occur at a level at which lexical information about the target is activated and retrieved. Here, we assess whether such an effect of syntactic variables (gender) also holds in experimental tasks designed to induce errors in unimpaired speakers of Italian (another gendered language) and whether this effect can also be observed in the semantic errors produced in the same task by AD patients. We found that for the unimpaired speakers, the gender of the target word constrained the error committed, while this was not the case for the AD patients. We take this finding to suggest a different locus for the errors in the two populations: while the semantic errors by the unimpaired speakers occur because of mis-selection of a lexical entry due to lexical competition among semantically similar words, the errors by the AD patients occur because of insufficient activation of lexical representations.