Microwave-assisted tissue processing, fixation and staining in tissues of different thicknesses: A comparative study.

AIM AND OBJECTIVES: The study aimed at assessment of microwave assisted tissue fixation, processing and staining and to determine if it can replace standard formalin fixed paraffin embedded processing in tissues of different thickness. MATERIALS AND METHODS: Specimens from buccal mucosa and gingiva was used in the study and were divided into three different thickness and was fixed, processed and stained according to conventional method and with a use of kitchen microwave oven respectively. The present study is the first of its kind where oral tissues was fixed, processed and stained with a kitchen microwave in three different thickness. The results obtained was statistically analyzed using IBM SPSS Statistics version 21.0 software. RESULTS: The new technique of fixation, tissue processing and staining using a microwave employed in the present study represented a major change from conventional method and achieved significant reduction in time taken. CONCLUSION: The ease of application and speed of this technique significantly reduced turnaround time in diagnostic labs.

Adenoid ameloblastoma with dentinoid: A rare hybrid variant.

Odontogenic tumors comprise an unusual group of lesions of the jaw and present diverse histological patterns. Derived from the primordial tooth-forming tissues, they represent a heterogeneous group of lesions that range from hamartomas to benign and malignant neoplasms of variable aggressiveness. Sporadic case reports and diverse complex histogenetic source also defy categorization of odontogenic tumors. Many can be diagnosed accurately based on the distinctive clinical, radiological and histopathological presentation. Considerable variations in the clinicopathological presentation of odontogenic tumors can be confusing, increasing the chance of misdiagnosis. An interesting case of adenoid ameloblastoma reported in a 55-year-old male patient in the mandible, presenting with a diverse and intriguing histopathology, is discussed here.

A rare incident of Paget's disease of bone in early adult life.

Paget's disease of bone (PDB) is characterized by a focal alteration of bone remodeling, which leads to bone with anomalous structure and altered mechanical properties associated with pain and complications. It is more common in Western Europe where as rare in Africa and Asia. The disease is more common in men of older adults and mean age of occurrence is 59 ± 14 years. But it is rarely encountered in patients younger than 40 years of age.