Assuntos
Carboxipeptidases/efeitos da radiação , Cininas/efeitos da radiação , Lisina Carboxipeptidase/efeitos da radiação , Peptidil Dipeptidase A/efeitos adversos , Raios Ultravioleta , Animais , Cininogênios/efeitos da radiação , Cininas/sangue , Lisina Carboxipeptidase/sangue , Masculino , Peptidil Dipeptidase A/sangue , Ratos , Ratos Endogâmicos , Fatores de TempoRESUMO
Traumatic shock was induced by the tourniquet method compressing one thigh during 10 hours. Venous blood samples were taken from control animals, as well as twice in the nervous phase of shock - after application and before removal of the tourniquet, and in the humoral-toxic phase - 1, 3 and 5 hours after tourniquet removal, in groups of 10 animals. Determinations included blood kinin level, and plasma kininogen level, and the activity of kallikreins and kininases in the plasma. It was found that during tourniquet shock a significant change occurred in the whole blood kinin system. Proportionally to the severity of shock the level of free kinins and kallikrein activity increased 3-4, times and the level of kininogen and the activity of kininases decreased, especially 3 hours after tourniquet removal.
Assuntos
Cininas/sangue , Choque Traumático/sangue , Animais , Hematócrito , Membro Posterior/irrigação sanguínea , Calicreínas/sangue , Cininogênios/sangue , Lisina Carboxipeptidase/sangue , Masculino , Peptidil Dipeptidase A/sangue , Coelhos , Choque Traumático/enzimologia , Choque Traumático/etiologia , TorniquetesRESUMO
The experiments were carried out on 54 rabbits in 9 groups of 6 animals each: group I -- controls, groups II, III, IV -- bradykinin i.v., groups V, VI, VII -- Depot-Kallikrein i.m. every other day for 3 weeks, groups VIII and IX -- Traskolan (trasylol) i.v. four times at intervals of 1 or 12 hours. The determined indices of cell-mediated and humoral immunity included: phagocytic activity of the reticuloendothelial system and peripheral blood leucocytes and their leukergic adhesiveness, haemagglutinin and haemolysin levels, serum complement titre, and the number of cells forming rosettes (RFC) or plaques (PFC) in the blood and spleen. These indices were determined 15 minutes, 3 and 24 hours after bradykinin administration, after 1, 2 and 3 weeks of kallikrein administration, and 1 or 12 hours after the last dose of Traskolan. Most determined indices showed always some fall. Only the phagocytic activity of the reticuloendothelial system was moderately increased in all groups, and in the bradykinin group leucocyte phagocytosis was increased slightly while their leukergic reaction was increased very strongly.
