[Effect of iodine and cobalt salts on content of biologically active substances in spirulina biomass]. / Vliianie solei ioda i kobal'ta na soderzhanie biologicheski aktivnykh veshchestv v biomasse spiruliny.

Production technology of iodinized spirulina biomass at vertical panel airlifted photobioreactor has been developed. The influence of different concentrations of iodine ions at certain combinations with cobalt ions on iodine-accumulative properties of spirulina has been studied. It has been shown that it is possible to obtain spirulina biomass containing certain amount of organically bound iodine by varying cobalt and iodine ions combinations and by staged addition of ions to incubation medium. This technique of staged addition of iodine and cobalt salt allowed to adopt spirulina to their high amount in the incubation medium, to increase spirulina productivity during growth, and to produce biomass with large iodine content.

[Effect of tocopherol and tocopherol quinone complexes with proteins on activity of leukotriene B4 lipoxygenase]. / Vplyv kompleksiv tokoferolu ta tokoferylkhinonu z bilkamy, shcho ïkh zv'iazuiut', na aktyvnist' leikotriien B4 lipoksyhenazy.

While estimating the effect of tocopherol, tocopherylquinone and their complexes with the tocopherol-binding proteins from the rat liver cytosole on arachidonate 5-lipoxygenase from peritoneal-lymphocytes and soybean linoleate-5-lipoxygenase alpha-tocopherol and especially its complex with tocopherol-binding protein was defined to inhibit the activity of both vegetative- and animal nature-lipoxygenase.

[Effect of the complexes tocopherol-protein-acceptors and tocopherylquinone-protein-acceptors on the activity cytosol phospholipase A2 in rat liver]. / Vliianie kompleksov tokoferol-belki-aktseptory i tokoferilkhinon-belki-aktseptori na aktivnost' fosfolipazy A2 tsitozolia pecheni krys.

A new method of tocopherol and tocopherylquinone binding with rat liver cytosol proteins-acceptors. Complexes tocopherol-proteins-acceptors and tocopheryl-quinone-proteins acceptors have been studied for their effect on phospholipase A2 activity. It is established that the both complexes are the inhibitors of phospholipase A2, under these conditions tocopherylquinone complex with proteins-acceptors more intensively decreases Km of the enzyme than the complex tocopherol-proteins-acceptors.

[In vitro incorporation of [14CH3]methionine into liver phosphatidylcholine in rats with vitamin E deficiency]. / Vkliuchenie in vitro [14CH3]metionina v fosfatidilkholin pecheni krys s E-gipovitaminozom.

The content of phosphatidyl choline, a terminal product of phosphatidyl ethanolamine methylation as well as in intensity in vitro incorporation of [14CH3]methionine into phosphatidyl choline of rat liver with E-hypovitaminosis are found to considerably decrease as compared to the norm. In this case the total content of phospholipids is practically the same.

[The role of vitamin E in the biosynthesis of the isoprenoid region of the ubiquinone molecule in the rat liver]. / Izuchenie roli vitamina E v biosinteze izoprenoidnoi chasti molekuly ubikhinona v pecheni krys.

A decrease in the CoA and acetyl-CoA amount in the rat liver tissue by 34.8 and 29.4%, respectively, as well as inhibition of the biosynthesis rate of mevalonic acid from [I-14C] acetyl-CoA in the postmitochondrial liver fraction by 17.9% as compared to the control are found against a background of E-hypovitaminosis. The last change is not associated with the inhibition of the 3-oxy-3-methylglutaryl-CoA-reductase activity and may not be one of reasons which cause the biosynthesis disturbances in the isoprenoid part of the ubiquinone molecule in this organ. alpha-Tocopherol activates the ubiquinone biosynthesis from [2-14C] sodium acetate in the liver of rats with E-hypovitaminosis under conditions of 30 min preincubation and is not efficient when added in combination with actinomycin D. Probably, such an effect of alpha-tocopherol is realized at the level of RNA synthesis and is associated with the biosynthesis activation of short-living RNA.

[Effect of alpha-tocopherol on relations between RNA and ubiquinone biosynthesis in rat liver]. / Vzaimosviaz' izmenenii biosinteza RNK i ubikhinona v pecheni krys pod vozdeistviem alpha-tokoferola.

The animals were kept on the low-protein diet deprived of vitamin E. Addition of the latter to the diet fed to a noticeable increase in the intensity of the biosynthesis of total and nuclear RNA of the rat liver. alpha-Tocopherol had a clearly marked stimulant effect on the synthesis of RNA and ubiquinone as shown by in vitro experiments under 30-minute preincubation of the liver from rats with E-hypovitaminosis. Meanwhile actinomycin D was found to inhibit these processes. Under preincubation with actinomycin D or concurrent introduction of the antibiotic with alpha-tocopherol the effect of the latter does no get realized. Unlike alpha-tocopherol, ionol and other synthetic antioxidants proved ineffective. It is assumed that the stimulant effect of vitamin E on the biosynthesis of ubiquinone in the liver of rats with E-hypovitaminosis is related to the increased intensity of the RNA synthesis and differs from the antioxidant one as regards the type. It is also assumed that the action of alpha-tocopherol on the RNA synthesis is effected at the transcriptional level.

[Alpha-tocopherol and actinomycin D regulation of ubiquinone and protein biosynthesis in rat liver in E-hypovitaminosis]. / Reguliatsiia al'fa-tokoferolom i aktinomitsinom D biosinteza ubikhinona i belka v pecheni krys pri E-gipovitaminoze.

In vitro experiments with the liver preparations from rats with E-hypovitaminosis revealed a more intensive incorporation of [14C]phenylalanine into ubiquinone induced by alpha-tocopherol. Actinomycin D inhibiting the DNA-dependent RNA synthesis decreases the incorporation intensity. A 30 min preincubation of the liver preparations with thesis. In case of preliminary addition of actinomycin D or its simultaneous introduction with alpha-tocopherol the latter has no stimulating effect on the intensity of [14C] phenylalanine incorporation into ubiquinone. When studying incorporation of [14C]phenylalanine into the total protein of the liver of rats with E-hypovitaminosis a tendency is revealed to an increase in the protein biosynthesis intensity in case of preincubation with alpha-tocopherol and to inhibition of the labelled precursor incorporation under conditions of preincubation with actinomycin D. The data obtained permit suggesting that the alpha-tocopherol effect on the ubiquinone biosynthesis may occur due to its effect on the RNA synthesis.

[Effect of vitamin E an actinomycin D on protein and RNA synthesis in rat liver]. / Biosintez belka i RNK v pecheni krys pod vozdeistviem vitamina E i aktinomitsina D.

In vitro experiments showed that RNA synthesis intensity in the rat liver with E-hypovitaminosis decreases considerably while the level of the labelled precursors incorporation into protein does not differ from the norm. Under conditions of E-hypovitaminosis the inhibitory effect of actinomycin D on the RNA synthesis is pronounced more clearly as compared to the norm. In the case of the E-hypovitaminous rate liver chyme preincubation with alpha-tocopherol there is no inhibitory effect of actinomycin D on the protein biosynthesis.