MiR-507 inhibits the progression of gastric carcinoma via targeting CBX4-mediated activation of Wnt/B-catenin and HIF-1α pathways

Purpose: Gastric carcinoma (GC) is a common malignant disease with high morbidity and mortality. MiR-507 has been confirmed as a tumor inhibitor which can suppress the progression of multiple cancers while its role in GC remains unknown.MethodsIn this study, the expression levels of miR-507 in the GC tissues and cells were observed by qRT-PCR, and CCK-8 assay, transwell asssay and TUNEL assay were used to observe the function of miR-507 on GC. The miRNA database and dual-luciferase reporter assay were used to investigate the downstream target of miR-507. Moreover, the activities of Wnt/β-catenin and HIF-1α pathways were observed by western blot.ResultsThe results showed that miR-507 was significantly downregulated in GC tissues and cell lines, and miR-507 upregulation effectively inhibited the proliferation and invasion and induced the apoptosis of GC cells. CBX4 was a downstream target of miR-507, and CBX4 could reverse the effects of miR-507 on the GC cells. Moreover, it was determined that miR-507 could inhibit CBX4 expression to suppress the activation of Wnt/β-catenin and HIF-1α pathways.ConclusionsIn conclusion, it suggests that miR-507 could inhibit the progression of GC via regulating CBX4-mediated activation of Wnt/β-catenin and HIF-1α pathways. (AU)

MiR-507 inhibits the progression of gastric carcinoma via targeting CBX4-mediated activation of Wnt/ß-catenin and HIF-1α pathways.

PURPOSE: Gastric carcinoma (GC) is a common malignant disease with high morbidity and mortality. MiR-507 has been confirmed as a tumor inhibitor which can suppress the progression of multiple cancers while its role in GC remains unknown. METHODS: In this study, the expression levels of miR-507 in the GC tissues and cells were observed by qRT-PCR, and CCK-8 assay, transwell asssay and TUNEL assay were used to observe the function of miR-507 on GC. The miRNA database and dual-luciferase reporter assay were used to investigate the downstream target of miR-507. Moreover, the activities of Wnt/ß-catenin and HIF-1α pathways were observed by western blot. RESULTS: The results showed that miR-507 was significantly downregulated in GC tissues and cell lines, and miR-507 upregulation effectively inhibited the proliferation and invasion and induced the apoptosis of GC cells. CBX4 was a downstream target of miR-507, and CBX4 could reverse the effects of miR-507 on the GC cells. Moreover, it was determined that miR-507 could inhibit CBX4 expression to suppress the activation of Wnt/ß-catenin and HIF-1α pathways. CONCLUSIONS: In conclusion, it suggests that miR-507 could inhibit the progression of GC via regulating CBX4-mediated activation of Wnt/ß-catenin and HIF-1α pathways.

Study on mobility-disadvantage group' risk perception and coping behaviors of abrupt geological hazards in coastal rural area of China.

China is a country highly vulnerable to abrupt geological hazards. The present study aims to investigate disaster preparedness and perception of abrupt geological disasters (such as rock avalanches, landslide, mud-rock flows etc) in mobility-disadvantage group living in coastal rural area of China. This research is to take into account all factors regarding disasters and to design the questionnaires accordingly. Two debris flow vulnerable townships are selected as study areas including Hedi Township in Qinyuan County and Xianxi Township in Yueqing City which are located in East China's Zhejiang Province. SPSS was applied to conduct descriptive analysis, which results in an effective empirical model for evacuation behavior of the disable groups. The result of this study shows mobility-disadvantage groups' awareness on disaster prevention and mitigation is poor and their knowledge about basic theory and emergency response is limited. Errors and distortions in public consciousness on disaster prevention and mitigation stimulate the development of areas with frequent disasters, which will expose more life and property to danger and aggravate the vulnerability of hazard bearing body. In conclusion, before drafting emergency planning, the government should consider more the disable group's expectations and actual evacuation behavior than the request of the situation to ensure the planning is good to work.

Interference with the ß-catenin gene in gastric cancer induces changes to the miRNA expression profile.

Aberrant activation of the Wnt/ß-catenin signaling pathway plays a major role in carcinogenesis and the progression of many malignant tumors, especially gastric cancer (GC). Some research has suggested that expression of the ß-catenin protein is associated with clinicopathologic factors and affects the biological behaviors of GC cells. However, the mechanism of these effects is not yet clear. Studies show that the Wnt/ß-catenin pathway regulates some miRNAs. We hypothesize that oncogenic activation of ß-catenin signaling is involved in the formation of GC through regulating certain microRNAs (miRNAs). The results of the current study demonstrate that expression of the ß-catenin protein is associated with many clinicopathologic characteristics including the degree of differentiation, depth of tumor invasion, tumor site, and 5-year survival rate. We found that silencing the expression of ß-catenin with lentiviruses could delay cell proliferation, promote apoptosis, weaken the invasive power of GC cells, and increase the sensitivity of GC cells to 5-fluorouracil in vitro. Using miRNA microarrays to detect changes in the miRNA transcriptome following interference with ß-catenin in GC cells, we found that miR-1234-3p, miR-135b-5p, miR-210, and miR-4739 were commonly upregulated and that miR-20a-3p, miR-23b-5p, miR-335-3p, miR-423-5p, and miR-455-3p were commonly downregulated. These data provide a theoretical basis for the potential interaction between miRNA and the ß-catenin signaling pathway in GC.