1.
J Am Chem Soc
; 137(49): 15398-401, 2015 Dec 16.
Artigo
em Inglês
| MEDLINE
| ID: mdl-26633100
RESUMO
Macrocyclization is a valuable tool for drug design and protein engineering. Although various methods have been developed to prepare macrocycles, a general and efficient strategy is needed. Here we report a highly efficient method using butelase 1 to macrocyclize peptides and proteins ranging in sizes from 26 to >200 residues. We achieved cyclizations that are 20,000 times faster than sortase A, the most widely used ligase for protein cyclization. The reactions completed within minutes with up to 95% yields.