Evaluation of the optimal strategy for ex situ bioremediation of diesel oil-contaminated soil.

PURPOSE: Bioaugmentation and biostimulation have been widely applied in the remediation of oil contamination. However, ambiguous results have been reported. It is important to reveal the controlling factors on the field for optimal selection of remediation strategy. In this study, an integrated field landfarming technique was carried out to assess the relative effectiveness of five biological approaches on diesel degradation. The limiting factors during the degradation process were discussed. METHOD: A total of five treatments were tested, including conventional landfarming, nutrient enhancement (NE), biosurfactant addition (BS), bioaugmentation (BA), and combination of bioaugmentation and biosurfactant addition (BAS). The consortium consisted of four diesel-degrading bacteria strains. Rhamnolipid was used as the biosurfactant. The diesel concentration, bacterial population, evolution of CO(2), and bacterial community in the soil were periodically measured. RESULTS: The best overall degradation efficiency was achieved by BAS treatment (90 ± 2%), followed by BA (86 ± 2%), NE (84 ± 3%), BS (78 ± 3%), and conventional landfarming (68 ± 3%). In the early stage, the total petroleum hydrocarbon was degraded 10 times faster than the degradation rates measured during the period from day 30 to 100. At the later stage, the degradation rates were similar among treatments. In the conventional landfarming, contaminated soil contained bacteria ready for diesel degradation. CONCLUSION: The availability of hydrocarbon was likely the limiting factor in the beginning of the degradation process. At the later stage, the degradation was likely limited by desorption and mass transfer of hydrocarbon in the soil matrix.

Ex situ bioremediation of oil-contaminated soil.

An innovative bioprocess method, Systematic Environmental Molecular Bioremediation Technology (SEMBT) that combines bioaugmentation and biostimulation with a molecular monitoring microarray biochip, was developed as an integrated bioremediation technology to treat S- and T-series biopiles by using the landfarming operation and reseeding process to enhance the bioremediation efficiency. After 28 days of the bioremediation process, diesel oil (TPH(C10-C28)) and fuel oil (TPH(C10-C40)) were degraded up to approximately 70% and 63% respectively in the S-series biopiles. When the bioaugmentation and biostimulation were applied in the beginning of bioremediation, the microbial concentration increased from approximately 10(5) to 10(6) CFU/g dry soil along with the TPH biodegradation. Analysis of microbial diversity in the contaminated soils by microarray biochips revealed that Acinetobacter sp. and Pseudomonas aeruginosa were the predominant groups in indigenous consortia, while the augmented consortia were Gordonia alkanivorans and Rhodococcus erythropolis in both series of biopiles during bioremediation. Microbial respiration as influenced by the microbial activity reflected directly the active microbial population and indirectly the biodegradation of TPH. Field experimental results showed that the residual TPH concentration in the complex biopile was reduced to less than 500 mg TPH/kg dry soil. The above results demonstrated that the SEMBT technology is a feasible alternative to bioremediate the oil-contaminated soil.