Phytoplankton community shift in response to experimental Cu addition at the elevated CO2 levels (Arabian Sea, winter monsoon).

Understanding phytoplankton community shifts under multiple stressors is becoming increasingly important. Among other combinations of stressors, the impact of trace metal toxicity on marine phytoplankton under the ocean acidification scenario is an important aspect to address. Such multiple stressor studies are rare from the Arabian Sea, one of the highest productive oceanic provinces within the North Indian Ocean. We studied the interactive impacts of copper (Cu) and CO2 enrichment on two natural phytoplankton communities from the eastern and central Arabian Sea. Low dissolved silicate (DSi < 2 µM) favoured smaller diatoms (e.g. Nitzschia sp.) and non-diatom (Phaeocystis). CO2 enrichment caused both positive (Nitzschia sp. and Phaeocystis sp.) and negative (Cylindrotheca closterium, Navicula sp., Pseudo-nitzschia sp., Alexandrium sp., and Gymnodinium sp.) growth impacts. The addition of Cu under the ambient CO2 level (A-CO2) hindered cell division in most of the species, whereas Chla contents were nearly unaffected. Interestingly, CO2 enrichment seemed to alleviate Cu toxicity in some species (Nitzschia sp., Cylindrotheca closterium, Guinardia flaccida, and Phaeocystis) and increased their growth rates. This could be related to the cellular Cu demand and energy budget at elevated CO2 levels. Dinoflagellates were more sensitive to Cu supply compared to diatoms and prymnesiophytes and could be related to the unavailability of prey. Such community shifts in response to the projected ocean acidification, oligotrophy, and Cu pollution may impact trophic transfer and carbon cycling in this region.

Interlinking diatom frustule diversity from the abyss of the central Arabian Sea to surface processes: physical forcing and oxygen minimum zone.

This study analyzed the diversity and abundance of diatom frustules including the ancillary parameters using the core top sediments from five locations (21, 19, 15, 13, and 11°N) along the central Arabian Sea (64°E), an area profoundly influenced by atmospheric forcing (monsoons) and oxygen minimum zone (OMZ) with high spatial variability. Significantly higher organic carbon (0.97 ± 0.05%) and diatom frustules (5.92 ± 0.57 × 104 valves g-1) were noticed in the north (21, 19, 15°N) where natural nutrient enrichment via open-ocean upwelling, winter convection, and lateral advection support large diatom-dominated phytoplankton blooms and intense OMZ. Conversely, the south (13, 11°N) depicted significantly lower organic carbon (0.74 ± 0.08%) as well as frustules (4.02 ± 0.87 × 104 valves g-1) as this area mostly remains nutrient-poor dominated by small-medium-sized phytoplankton. The north was dominated by large-sized diatoms like Coscinodiscus that could escape grazing and sink consequently due to higher ballasting. Furthermore, the presence of the intense OMZ in the north might reduce grazing pressure (low zooplankton stock) and mineralization speed facilitating higher phytodetritus transport. Relatively smaller chain-forming centric (Thalassiosira) and pennate diatoms (Pseudo-nitzschia, Fragilaria, Nitzschia, etc.) were found throughout the transect with higher abundance in the south. The euphotic diatom diversity from the existing literature was compared with the frustule diversity from the sediments suggesting not all diatoms make their way to the abyss. Such distinct spatial north-south variability in diatom frustule size as well as abundance could be attributed to cell size, grazing, and water column mineralization rates related to OMZ.