The bovine protein alpha-lactalbumin increases the plasma ratio of tryptophan to the other large neutral amino acids, and in vulnerable subjects raises brain serotonin activity, reduces cortisol concentration, and improves mood under stress.

BACKGROUND: Increased brain serotonin may improve the ability to cope with stress, whereas a decline in serotonin activity is involved in depressive mood. The uptake of the serotonin precursor, tryptophan, into the brain is dependent on nutrients that influence the cerebral availability of tryptophan via a change in the ratio of plasma tryptophan to the sum of the other large neutral amino acids (Trp-LNAA ratio). Therefore, a diet-induced increase in tryptophan availability may increase brain serotonin synthesis and improve coping and mood, particularly in stress-vulnerable subjects. OBJECTIVE: We tested whether alpha-lactalbumin, a whey protein with a high tryptophan content, may increase the plasma Trp-LNAA ratio and reduce depressive mood and cortisol concentrations in stress-vulnerable subjects under acute stress. DESIGN: Twenty-nine highly stress-vulnerable subjects and 29 relatively stress-invulnerable subjects participated in a double-blind, placebo-controlled study. Subjects were exposed to experimental stress after the intake of a diet enriched with either alpha-lactalbumin or sodium-caseinate. Diet-induced changes in the plasma Trp-LNAA ratio and prolactin were measured. Changes in mood, pulse rate, skin conductance, and cortisol concentrations were assessed before and after the stressor. RESULTS: The plasma Trp-LNAA ratio was 48% higher after the alpha-lactalbumin diet than after the casein diet (P = 0.0001). In stress-vulnerable subjects this was accompanied by higher prolactin concentrations (P = 0.001), a decrease in cortisol (P = 0.036), and reduced depressive feelings (P = 0.007) under stress. CONCLUSIONS: Consumption of a dietary protein enriched in tryptophan increased the plasma Trp-LNAA ratio and, in stress-vulnerable subjects, improved coping ability, probably through alterations in brain serotonin.

Body size perception in anorexia nervosa: a signal detection approach.

This study investigated whether individuals with anorexia nervosa (AN) visualize themselves as fatter than they are because they perceive themselves as fatter. Females with AN who overestimated their own body size judged size differences between pictures of their own body, and then again of someone else's body. Signal detection analysis of the results showed no differences in perceptual sensitivity between the AN and normal and thin control groups. No significant correlations were found between body size estimates and perceptual sensitivity. The anorexic group did, however, show a bias to report seeing "thin" differences, which was opposite to that of thin controls. Because bias differences between the groups were significant while sensitivity differences were not, it was concluded that abnormalities of body image most probably arise during reconstruction of the visual body image, rather than during perception of the body.

Body perception index: benefits, pitfalls, ideas.

Estimates of body size are often expressed as a ratio of actual size [body perception index or BPI = (estimated size/actual size) x 100%]. In this article, we examine the possibility that overestimation of body size in patients with anorexia nervosa, as measured by the BPI, is due to their smaller body size rather than to their being anorexic. Using 50 mean body sizes derived from seven studies we investigated whether the error of estimation is a constant proportion of the body size to be estimated, as the use of the BPI assumes. A negative linear relation between BPI and actual body size was found, confirming that smaller size is associated with greater overestimation. However, although both groups showed a strong tendency to overestimate smaller sizes, anorexic subjects showed even greater overestimation than controls. Hence, overestimation of body size in AN can only partially be accounted for by the smaller body size of anorexic patients. Recommendations for future use of the BPI are put forward.

The influence of methodological differences on the outcome of body size estimation studies in anorexia nervosa.

A major problem that has dogged research into body image disturbance in anorexia nervosa concerns the diversity of measurement procedures employed in studies of body size estimation. Many researchers believe that results obtained with different procedures cannot meaningfully be compared with each other, because the methods used measure different aspects of the body image. A meta-analysis of 33 body size estimation studies was conducted to investigate whether methodological differences do indeed influence outcome. This analysis revealed a general overestimation of body size among anorexia patients. Although a difference in mean effect size was found between studies using Body Part and Whole Body methods, this difference was absent when only the most commonly used Body Part method, the visual size estimation procedure, was considered. Among Whole Body methods, silhouette methods could not be distinguished from the rest in terms of effect size. It was concluded that Whole Body methods and the visual size estimation procedure assess correlated aspects of the body image.