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Am J Bot ; 98(3): e54-7, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-21613124

RESUMO

PREMISE OF THE STUDY: Traditional taxonomy and nomenclature of Brassiceae (Brassicaceae) species do not reflect their phylogeny. Revision of the species and generic limits supported by extensive molecular data seems crucial. METHODS AND RESULTS: Genome-specific polymorphisms extracted from non-coding and coding sequences were used to develop 14 sequence characterized amplified region (SCAR) markers specific for the Brassica B genome. These SCARs were verified against 77 accessions of six U-triangle Brassica species and used to screen 23 accessions of seven wild Brassiceae species to test for their cross-species amplification. SCARs were found in all B-genome Brassica species and also in Sinapis arvensis. CONCLUSIONS: SCAR markers can be employed for discerning B-genome chromosomes in Brassica species and S. arvensis to reliably identify B-genome species and their natural hybrids. The combined molecular evidence supports the suggestion to revise the generic limits of Brassica and Sinapis.


Assuntos
Genoma de Planta/genética , Filogenia , Sinapis/classificação , Sinapis/genética , Marcadores Genéticos , Testes Genéticos , Especificidade da Espécie
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