[Neurohormonal regulation of the development and regenerative processes of bone and cartilage tissues]. / Neirogormonal'naia reguliatsiia razvitiia i vosstanovitel'nykh protsessov kostnoi i khriashchevoi tkanei.

Neurohumoral regulation of cartilaginous and osseous tissues was investigated in different stages of ontogenesis. It has been shown that these tissues are targets practically for all hormones produced by the body. The reciprocal reaction of osseous and cartilaginous tissues to different concentrations of hormones was estimated. It has been also demonstrated that the action of hormones in these tissues manifests itself in accordance with the general regularities and that their effects are strictly specific for the test target tissues. An opinion is advanced about the breach of the endocrine situation in the body as a risk factor of the development of dystrophic processes in cartilages.

[Genetically determined diseases of the hip joint (a conceptual model)]. / Nasledstvenno predraspolozhennye zabolevaniia tazobedrennogo sustava (kontseptual'naia model').

The authors consider the problem of pathology of the hip joint from the point of view of the conception "Dysplasia-coxarthrosis". A single system of the ideas concerning dysplastis pathology is presented, which will allow to unify the methods of diagnosis and treatment of dysplasia of the hip joint in children, adolescents and adults with the aim of prevention of crippling diseases of the joint.

[Age-related changes in the intercellular substance of human hyalin and collagen-fiber cartilage tissue]. / Vozrastnye izmeneniia mezhkletochnogo veshchestva gialinovoi i kollagenovoloknistoi khriashchevoi tkani cheloveka.

The articular cartilage of the hip joint and the intervertebral disks (LIV and LV), obtained from 42 corpses without any signs of pathology in these tissues, have been investigated. Six age groups have been distinguished: 21-30, 31-40, 41-50, 51-60, 61-70, 71 years of age and older. After the topo-optic reactions have been get, double refraction of glycosamineglycans (GAG) and collagen is studied in the structure and orientational regularity is estimated in these macromolecules in the matrix. The peculiarities on distribution of the collagenous fiber fasciculi in the intercellular substance in the hyalin and collagen-fibrillar cartilaginous tissue are demonstrated at the areas and depending on the age. Correlations in qualitative and quantitative changes of the GAG and collagen with age are analysed; this gives certain possibilities to reveal signs of similarity and difference in morphogenesis of hyalin and collagen-fibrillar cartilage tissue. Basing on the comparative analysis performed concerning the GAG and collagen state in the age aspect, as well as taking into account the data of previously performed investigations, the collagenous carcass can be characterized as the most stable component of the intercellular substance of cartilages, possessing a great reserve of strength. Homokinesis of the cartilaginous tissue is performed at the expense of the GAG; this ensures a high ability of the cartilaginous tissue to adaptation. A hypothesis on the GAG role in realization of adaptive rearrangements of the cartilaginous matrix at the macromolecular level is put forward.

[Scanning electron microscopy of the surface of Chinese hamster fibroblast-like cells following glycerin treatment]. / Rastrovaia élektronnaia mikroskopiia poverkhnosti fibroblasto-podobnykh kletok kitaiskogo khomiachka posle obrabotki glitserinom.

The effect of glycerol upon the superficial structure of Chinese hamster fibroblast-like cells was studied. The analysis of scanning electron microscopy data made it clear that glycerol-induced changes in the surface structures were of various character depending on the duration of the contact period. A long-term (4 hour long) exposure to glycerol provoked significant structural changes. Nevertheless, the effects were reversible, judging by the fact that the washing-out of cell suspensions led to the repair of cell structures.

[Principles of polarization-optical analysis in the study of connective tissue]. / Printsipy poliarizatsionno-opticheskogo analiza v izuchenii soedinitel'noi tkani.

Possibilities of polarization-optic methods for studying macromolecular organization of the intercellular matrix of the connective tissue are demonstrated. Topo-optic reactions, being histochemical, make it possible to reveal not only presence of the molecules studied, but also quantitatively determine degree of their orientational regularity in the structural organization of the intercellular matrix. Determination of changes in the orientational regularity of single molecules contributes to revealing morphogenetic peculiarities of the intercellular matrix of the connective tissue, normal and under development of the destructive-distrophic process. Methodological principles of reactions to collagen, glycosaminoglycanes, glycoproteins, elastin and non-collagenous proteins are presented. Their advantages comparing certain known histochemical reactions, as well as their limitations are demonstrated. Certain illustrative material is presented.

[Stathmokinetic effect of cryoprotectors]. / Statmokineticheskii éffekt krioprotektorov.

A study was made of the effect of cryoprotectors, both penetrating the cell (glycerin) and non-penetrating it (polyethylene oxide, m.w. 400), in different concentrations on the mitosis kinetics of short-lived cultures of human peripheral blood cells and continuous fibroblast-like cells of Chinese hamsters. Changes in the kinetics of the proliferation of the cultures under study, manifesting in a decrease of the mitotic index, accumulation of metaphases and an increase in the number of k-mitotic cells, depended on the concentration of the cryoprotectors. The stathmokinetic effect of the cryoprotectors was discovered to be related to the dehydrating properties shared by these substances, with the intensity of the effect being consequent on their penetrating ability.