[Heterogeneity of Escherichia coli population during induced autolysis]. / Geterogennost' populiatsii Escherichia coli v protsesse indutsirovannogo avtoliza.

Escherichia coli M-17 autolysis was induced by eliminating nutrition sources from the growth medium and exerting a shock with EDTA. The overall cell number, the optical density of the cell suspension, the number of colony-forming units (CFU), and [3H]uracil incorporation into the cells were analysed in the course of autolysis. The number of CFU was found to drop down faster than the overall cell number in the process of autolysis. The population of E. coli was shown to be heterogeneous in its sensitivity to the induction of autolysis, and some nonlysed cells were still metabolically active. When the rate of autolysis was highest in some cells of the population, the labeled precursor was found to be incorporated into the TCA-soluble and TCA-insoluble fractions of nonlysed cells. The overall cell number, the optical density of the cell suspension, and the number of CFU increased 96 h after the induction of autolysis. The authors discuss what is the role played by the heterogeneity of an E. coli population in its adaptation to EDTA-induced autolysis.

[Heterogeneity of the population of Escherichia coli thr- by assimilation of 3H-threonine]. / Geterogennost' populiatsii Escherichia coli thr- po usvoeniiu 3H-treonina.

The incorporation and spatial localisation of [3H]threonine in the cells of Escherichia coli RP 477 (leu-his-thr-B1-) were studied using the techniques of radiometry and adsorption electron-microscopic autoradiography with additional time-lapse microfilming to follow up the growth of the population. The strain was shown to be heterogeneous in its metabolic, reproductive and morphological (cell diameter) characteristics; a generative cluster was revealed. Indirect data indicated that the so-called "resting" cells were functionally active.

[Cortisol level and immunosuppression in viral hepatitis B in children and the approach to its treatment with glucocorticoids]. / Urove'n kortizola i dinamika immunodepressii pri virusnom gepatite B u detei i podkhody k lecheniiu gliukokortikoidami.

As many as 132 children with verified virus hepatitis were examined. Cortisol content in blood serum was measured and the parameters of cellular-humoral immunity were determined over time in the acute period and in the catamnesis up to 1 year. In the cyclic self-limiting forms of hepatitis B, the children developed immunologic unbalance with progressive hypercortisolemia, being an adequate reaction of the stressor systems and not requiring prednisolone administration. In protracted and chronic persistent hepatitis, the children developed immunosuppression and monotonous hypercortisolemia requiring short-term prednisolone treatment. The children afflicted with the grave and malignant forms of hepatitis B demonstrated, within the early times of the disease, secondary immunodeficiency and a considerable drop of cortisol content, demanding early prednisolone administration.

[Growth dyssymmetry of Shigella flexneri 6(762 S) cells]. / Dissimmetriia rosta kletok Shigella flexneri 6(762 S).

Dyssymmetry in the growth of the initial and secondary shigella cells at early stages of the formation of microcolonies has been revealed by the method of dynamic morphometry in serial photographs obtained by time-lapse microfilming. Electron-microscopic autoradiography has allowed revealing dyssymmetry in the assimilation of exogenous H3-thymidine, H3-threonine, H3-asparaginic acid by different halves of shigella cells in the process of their growth and preparation for mytosis. These results suggest that the shigellae are divided into two unequal cells: the "mother" cell and the "daughter" one.

[Plastic repair of the urinary bladder]. / K voprosu o plastike mochevogo puzyria

Clinical observations of 76 patients in whom an artifical urinary bladder was constructed from the rectum are presented. 52 patients were operated upon for cancer of the urinary bladder, total papillomatosis, a metastasis of uterine cancer in the bladder, cancer of the sigmoid with the urinary bladder involvement, sarcoma and cancer of the prostate with the bladder involvement. 17 patients were operated upon for extrophy, 7 -- for cancer of the urethra. An isolation of the rectum is followed by ligation of the upper rectal and vein. Fifteen patients died postoperatively due to peritonitis (6), ascending infection of the urinary tract (4), postoperative shock (I), phlegmon of the minor pelvis (I). In late postoperative period an artificial urinary bladder provides for reservoir and excretory functions.