RESUMO
AIM: To investigate the use of Daclizumab (Dmab) as an immunosuppressive agent in an experimental model of hepatocyte xenotransplantation (XenoTx) in rats with fulminant hepatic failure (FHF). METHODS: Two white male New Zealand rabbits were used as donors and 68 Wistar rats as recipients. FHF was induced by intravenous application of dimethylnitrosamine (DMNA). The isolated hepatocytes of the rabbits were xenotransplanted into the spleen of the rats 24 h after FHF induction. Group A (n = 13): no treatment; Group B (n = 14): FHF and XenoTx; Group C (n = 14): FHF and XenoTx and cyclosporin (CsA); Group D (n = 14): FHF and XenoTx and Dmab; Group E (n = 13): FHF and XenoTx and CsA and Dmab. The rats were followed for 15 d. RESULTS: Statistical analysis showed better survival among groups D (92.86%) and E (76.92%) compared to group A (all rats died after 72 h), group B (28.57%) or group C (71.43%), although the differences were not statistically significant. Biochemical evaluation of the liver enzymes and histology confirmed satisfactory function and engraftment, respectively. CONCLUSION: This experimental model has shown the safe, effective and beneficial use of Dmab in a xenotransplantation model of rabbit hepatocytes in rats.
Assuntos
Anticorpos Monoclonais/uso terapêutico , Hepatócitos/transplante , Imunoglobulina G/uso terapêutico , Imunossupressores/uso terapêutico , Falência Hepática Aguda/cirurgia , Receptores de Interleucina-2/antagonistas & inibidores , Transplante Heterólogo/métodos , Doença Aguda , Animais , Anticorpos Monoclonais/imunologia , Anticorpos Monoclonais Humanizados , Transplante de Células/métodos , Daclizumabe , Modelos Animais de Doenças , Imunoglobulina G/imunologia , Falência Hepática Aguda/patologia , Masculino , Coelhos , Ratos , Ratos Wistar , Receptores de Interleucina-2/imunologia , Linfócitos T Auxiliares-Indutores/imunologia , Linfócitos T Auxiliares-Indutores/patologia , Transplante Heterólogo/imunologiaRESUMO
Colonic lesions of benign or early malignant origin may be difficult to remove by colonoscopy. However, conventional surgical resection is considered as too invasive for these types of lesions suitable for local excision. The combined laparoscopic-colonoscopic excision was performed in 75 patients (males, 42; females, 33) with benign or early malignant lesions of the colorectum. Three different variations were used: laparoscopy-assisted endoscopic resection (LAER), endoscopy-assisted laparoscopic wedge resection (EAWR), and endoscopy-assisted laparoscopic transluminal resection (EATR). If these techniques were not applicable, an endoscopy-assisted laparoscopic segment resection (EASR) was performed. Conversion rate was 5.0%. Although the expenditure of combined endoscopic/laparoscopic approaches is higher, they are attractive alternatives to either laparoscopic tubular resection or open surgery.
