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1.
Bone Marrow Transplant ; 48(11): 1421-8, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23665821

RESUMO

Intestinal acute GVHD (I-aGVHD) is a life-threatening complication after allografting. Non-invasive bed-side procedures to evaluate extension and treatment response are still lacking. We hypothesized that, during I-aGVHD, contrast-enhanced ultrasound sonography (CEUS) could detect microcirculation changes (MVC) of the bowel wall (BW) and help to monitor treatment response. We prospectively employed CEUS in 83 consecutive patients. Of these, 14 patients with biopsy-proven intestinal GVHD (I-GVHD) were defined as the study group, whereas 16 patients with biopsy-proven stomach GVHD (U-GVHD) without intestinal symptoms, 6 normal volunteers and 4 patients with neutropenic enterocolitis were defined as the control group. All patients were evaluated with both standard ultrasonography (US) and CEUS at the onset of intestinal symptoms, during clinical follow-up and at flare of symptoms. Standard US revealed BW thickening of multiple intestinal segments, useful to determine the extension of GVHD. CEUS showed MVC, which correlated with GVHD activity, treatment response, and predicted flare of intestinal symptoms. US and CEUS findings were superimposable at diagnosis and in remission. CEUS was, however, more sensitive and specific to identify subclinical activity in patients with clinical relevant improvement. These findings were not observed in the control groups. CEUS is a non-invasive, easily reproducible bed-side tool useful to monitor I-aGVHD.


Assuntos
Doença Enxerto-Hospedeiro/diagnóstico por imagem , Enteropatias/diagnóstico por imagem , Doença Aguda , Adulto , Idoso , Estudos de Casos e Controles , Feminino , Doença Enxerto-Hospedeiro/imunologia , Humanos , Enteropatias/imunologia , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Transplante Homólogo/métodos , Ultrassonografia , Adulto Jovem
2.
J Chemother ; 21(3): 311-6, 2009 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19567352

RESUMO

In randomized studies linezolid, indicated for Gram-positive infections, was as effective as teicoplanin in critical ill patients or was superior to teicoplanin in skin infection, pneumonia and bacteremia. We performed a 2-year comparative, retrospective study of patients treated with linezolid or teicoplanin in a single hospital for the same indications. We collected information about the type of infection, the responsible pathogen, therapy administered before study drugs, antibiotic associated with the study drugs, length of hospital stay (LOS), adverse events and outcome of the infections. The aim of the study was to evaluate the efficacy of linezolid in this retrospective patients series. Overall we identified 169 patients treated with linezolid and 91 with teicoplanin. Response to therapy, (resolution or improvement of infection) was better in patients treated with linezolid compared to teicoplanin (83.9% versus 69.2%, p=0.002). Response to therapy by type of pathogen showed the superior efficacy of linezolid against Staphylococcus aureus (including MRSA) and enterococci; although not statistically significant because of the small number of patients enrolled, they were close to significance (p<0.056 for S. aureus, p<0.055 for MRSA, p<0.061 for enterococci). Overall LOS in linezolid-treated patients was 4.6 days (p<0.041) less. Empirical use of linezolid reduced lOS by 6 days (p<0.038), especially in VAP and bacteremia patients (p<0.05). Mortality due to infection was 9.8% in both groups, and adverse events were most frequently documented in linezolid-treated patients. Linezolid was clinically superior to teicoplanin in the treatment of Gram-positive infections.


Assuntos
Acetamidas/uso terapêutico , Anti-Infecciosos/uso terapêutico , Infecções por Bactérias Gram-Positivas/tratamento farmacológico , Oxazolidinonas/uso terapêutico , Teicoplanina/uso terapêutico , Acetamidas/efeitos adversos , Adulto , Idoso , Feminino , Humanos , Tempo de Internação , Linezolida , Masculino , Pessoa de Meia-Idade , Oxazolidinonas/efeitos adversos , Pneumonia Bacteriana/tratamento farmacológico , Pneumonia Associada à Ventilação Mecânica/tratamento farmacológico , Estudos Retrospectivos , Teicoplanina/efeitos adversos
4.
J Ultrasound ; 11(3): 97-101, 2008 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23396752

RESUMO

INTRODUCTION: Neutropenic enterocolitis (NEC) can be a life-threatening complication of chemotherapy in leukemic patients. Early diagnosis and treatment is therefore crucial. METHODS: A 38-year-old woman with acute lymphoblastic leukemia and chemotherapy-induced neutropenia suddenly developed symptoms suspicious of NEC. Transabdominal ultrasound showed features consistent with NEC, later confirmed by computed tomography (CT) scan. RESULTS: The patient was scanned using portable ultrasound (US) equipment (Esaote My Lab 25). US findings showed involvement of the cecum, appendix, ascending colon and proximal middle transverse colon, with features resembling gas containing fissures within the colon wall itself. The risk of colon rupture was confirmed by CT scan. The patient underwent successful hemicolectomy after intravenous treatment with broad spectrum antibiotics, granulocyte-colony stimulating factor (G-CSF), platelets and fresh frozen plasma transfusion. DISCUSSION: A prompt bedside US examination upon development of symptoms allowed an early diagnosis of NEC and identified features consistent with imminent colon wall rupture, shifting the management of this life-threatening complication from medical to surgical. Multidisciplinary intervention was crucial for a successful hemicolectomy in a severely affected neutropenic patient.

8.
Biomed Pharmacother ; 59(9): 511-6, 2005 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-16274955

RESUMO

The infectious complications are an important cause of morbidity and mortality in hematopoietic stem cell transplant (HSCT) recipients. Our retrospective study has the objective to evaluate the incidence, clinical and bacteriologic features of documented infections in these patients. The frequency of infectious complications was analysed in 42 patients with hematologic malignancies who received HSCT from January to December 2002 at Pisa General Hospital. Thirty-three patients underwent autologous HSCT and 9 received allogeneic HSCT. All patients received acyclovir, fluconazole and fluoroquinolones as prophylactic regimen. A total of 38 infectious episodes were recognized in 22 patients during the early post-HSCT period (N=27) and in the late post-HSCT period (N=11). Infectious complications rate correlated positively with the deepness and length of neutropenia in the early period. There were 21 episodes of sepsis (the majority by coagulase negative staphylococci), 2 pneumonias and 1 vertebral osteomyelitis. All staphylococcus strains were, in vitro, resistant to oxacillin and ciprofloxacin and 8 out of 15 gram negative rods were resistant to ciprofloxacin. Most of the infectious complications were cured with appropriated antimicrobial therapy and/or with engraftment and, in 4 cases, with central catheter removal. One patient developed a positive CMV antigenemia; a pre-core mutant form of HBV reactivation was diagnosed in another patient. No cases of invasive fungal infections were recognised. Five patients died but only one from infection (septic shock). Pneumonia was a coexisting cause of death in 2 patient in the late period. We can conclude that most of infectious complications, that occurred in the early period post-HSCT were due to coagulase negative staphylococci and gram negative rods resistant to ciprofloxacin. For this reason, the usefulness of fluoroquinolone prophylaxis in HSCT recipients should be reevaluated.


Assuntos
Antifúngicos/uso terapêutico , Infecções Bacterianas/complicações , Resistência a Medicamentos/efeitos dos fármacos , Fluoroquinolonas/uso terapêutico , Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Complicações Pós-Operatórias/microbiologia , Adulto , Idoso , Infecções Bacterianas/tratamento farmacológico , Infecções Bacterianas/epidemiologia , Infecções Bacterianas/etiologia , Infecções Bacterianas/mortalidade , Cateteres de Demora/microbiologia , Feminino , Infecções por Bactérias Gram-Negativas/epidemiologia , Infecções por Bactérias Gram-Positivas/epidemiologia , Neoplasias Hematológicas/tratamento farmacológico , Transplante de Células-Tronco Hematopoéticas/mortalidade , Humanos , Incidência , Itália/epidemiologia , Masculino , Pessoa de Meia-Idade , Neutropenia/microbiologia , Pneumonia/microbiologia , Pneumonia/mortalidade , Estudos Retrospectivos , Sepse/microbiologia , Sepse/mortalidade , Fatores de Tempo , Transplante Autólogo/estatística & dados numéricos , Transplante Homólogo/estatística & dados numéricos , Viroses/complicações , Viroses/tratamento farmacológico
10.
Bone Marrow Transplant ; 33(6): 659-60, 2004 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-14755324

RESUMO

A 55-year-old female with standard risk AML in second CR received an allogenic transplant from an HLA-matched sibling, using a nonmyeloablative conditioning regimen (NMST). On day +139, she rejected her graft with autologous reconstitution. She received a second NMST from a different HLA-matched sibling with an identical conditioning regimen and immunosuppression. On day +110, she rejected the second graft, with autologous reconstitution with blasts. She received a third allograft from the first sibling with a myeloablative busulfan-based conditioning regimen. She is now day +270, in CR, with full donor chimerism.


Assuntos
Rejeição de Enxerto/imunologia , Leucemia Mieloide Aguda/terapia , Transplante de Células-Tronco , Transplante Homólogo/imunologia , Feminino , Teste de Histocompatibilidade , Humanos , Pessoa de Meia-Idade , Quimeras de Transplante , Condicionamento Pré-Transplante/métodos , Resultado do Tratamento
11.
Bone Marrow Transplant ; 32(1): 57-63, 2003 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-12815479

RESUMO

The main aim of this paper was to compare results of Genescan and real-time PCR methods in order to detect contamination in harvests from patients with follicular lymphoma. The secondary goal was to evaluate the efficacy of Rituximab as an in vivo purging agent. A total of 23 patients had been treated with CHOP followed by either high-dose therapy (12 patients) or high-dose plus Rituximab (11 patients), both followed by autologous transplantation. Results show that 86% of harvests from patients treated with Rituximab were PCR-negative compared to 14.3% from controls. Real-time PCR was more sensitive than Genescan PCR; quantitative analysis revealed a correlation between the amount of contamination in the harvests and relapse after transplantation. Whereas all patients reinfused with negative aphereses achieved complete remission and showed a significantly better 5-year PFS (100%) compared to those reinfused with contaminated samples (41%), a very low amount of contamination does not appear to negatively affect outcome, suggesting that determination of a cutoff in the contamination level of harvests could be useful. Results suggest that real-time PCR is superior to Genescan PCR to select transplantable harvests and confirm the ability of Rituximab as an in vivo purging tool for follicular lymphoma.


Assuntos
Anticorpos Monoclonais/uso terapêutico , Linfoma Folicular/terapia , Células Neoplásicas Circulantes/efeitos dos fármacos , Transplante de Células-Tronco de Sangue Periférico/métodos , Reação em Cadeia da Polimerase/normas , Adulto , Anticorpos Monoclonais/administração & dosagem , Anticorpos Monoclonais Murinos , Intervalo Livre de Doença , Feminino , Rearranjo Gênico , Genes bcl-2 , Humanos , Leucaférese/métodos , Leucaférese/normas , Linfoma Folicular/diagnóstico , Masculino , Pessoa de Meia-Idade , Técnicas de Diagnóstico Molecular , Transplante de Células-Tronco de Sangue Periférico/normas , Reação em Cadeia da Polimerase/métodos , Rituximab , Sensibilidade e Especificidade , Transplante Autólogo
12.
J Neurol ; 249(8): 1088-97, 2002 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-12195460

RESUMO

RATIONALE: Phase I/II studies of autologous hematopoietic stem cell transplantation (HSCT) for multiple sclerosis ( MS) were initiated, based on results of experimental transplantation in animal models of multiple sclerosis and clinical observations in patients treated concomitantly for malignant disease. PATIENTS: Eighty-five patients with progressive MS were treated with autologous HSCT in 20 centers and reported to the autoimmune disease working party of the European Group for Blood and Marrow Transplantation (EBMT). 52 (61 %) were female, median age was 39 [20-58] years. The median interval from diagnosis to transplant was 7 [1-26] years. Patients suffered from severe disease with a median EDSS score of 6.5 [4.5-8.5]. Active disease prior to transplant was documented in 79 of 82 evaluable cases. RESULTS: The stem cell source was bone marrow in 6 and peripheral blood in 79, and stem cells were mobilized into peripheral blood using either cyclophosphamide combined with growth factors or growth factors alone. Three patients experienced transient neurological complications during the mobilization phase. The high dose regimen included combination chemotherapy, with or without anti-lymphocyte antibodies or, with or without, total body irradiation. The stem cell transplants were purged of lymphocytes in 52 patients. Median follow-up was 16 [3-59] months. There were 7 deaths, 5 due to toxicity and infectious complications, 2 with neurological deterioration. The risk of death of any cause at 3 years was 10 (+/-7)% (95 % confidence interval). Neurological deterioration during transplant was observed in 22 patients; this was transient in most but was associated with MS progression in 6 patients. Neurological improvement by > or = 1 point in the EDSS score was seen in 18 (21 %) patients. Confirmed progression-free survival was 74 (+/-12)% at 3 years being 66 (+/-23)% in patients with primary progressive MS but higher in patients with secondary progressive or relapsing-remitting MS, 78 (+/-13)%; p = 0.59. The probability of confirmed disease progression was 20 (+/-11)%. MRI data were available in 78 patients before transplant showing disease activity (gadolinium enhancing, new or enlarging lesions) in 33 %. Posttransplant MRI showed activity at any time in 5/61 (8 %) evaluable cases. CONCLUSION: Autologous HSCT suggest positive early results in the management of progressive MS and is feasible. These multicentre data suggest an association with significant mortality risks especially in some patient groups and are being utilised in the planning of future trials to reduce transplant related mortality.


Assuntos
Transplante de Células-Tronco Hematopoéticas , Esclerose Múltipla Crônica Progressiva/terapia , Condicionamento Pré-Transplante , Adulto , Transplante de Medula Óssea/efeitos adversos , Transplante de Medula Óssea/mortalidade , Contagem de Linfócito CD4 , Progressão da Doença , Intervalo Livre de Doença , Feminino , Mobilização de Células-Tronco Hematopoéticas/efeitos adversos , Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Transplante de Células-Tronco Hematopoéticas/mortalidade , Humanos , Masculino , Pessoa de Meia-Idade , Esclerose Múltipla Crônica Progressiva/imunologia , Esclerose Múltipla Crônica Progressiva/mortalidade , Estudos Retrospectivos , Condicionamento Pré-Transplante/efeitos adversos , Resultado do Tratamento
13.
Bone Marrow Transplant ; 29(7): 581-7, 2002 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11979307

RESUMO

Seventy-two patients with non-Hodgkin's lymphoma were evaluated for the presence of molecular markers (IgH, bcl-1, bcl-2 rearrangement) on bone marrow, at diagnosis and after PBSCT, and on harvests in order to find a possible predictive role of minimal residual disease on treatment outcome. At diagnosis, 41 (59%) out of 69 available bone marrows showed molecular involvement. Fifty-six percent of leukaphereses were involved, mainly indolent lymphoma (P = 0.001) or advanced disease (P = 0.01). Ex vivo purging cleared only one stem collection out of 31 PCR-positive leukaphereses. Aggressive lymphomas showed both a longer overall survival (OS) (P = 0.03) and relapse-free survival RFS (P = 0.02) when transplanted with unpurged stem cells, whereas indolent NHL survival was not influenced by ex vivo purging. Twenty out of 26 samples taken during follow-up had bone marrow involvement at diagnosis. Of these, 15 cleared their bone marrow; both OS and RFS were significantly longer in the PCR-negative cases (P = 0.05 and P = 0.005). At 1 year after PBSCT, 75% of patients were PCR negative, with 50% molecular remissions; the relapse rate was 55% for patients still PCR positive vs 29% for those who were PCR negative. Thus, after high-dose chemotherapy, close molecular monitoring of MRD using qualitative PCR techniques seems to represent a reliable prognostic indicator.


Assuntos
Biomarcadores Tumorais/análise , Medula Óssea/química , Ciclina D1/análise , Cadeias Pesadas de Imunoglobulinas/análise , Linfoma não Hodgkin/química , Proteínas de Neoplasias/análise , Proteínas Proto-Oncogênicas c-bcl-2/análise , Adolescente , Adulto , Protocolos de Quimioterapia Combinada Antineoplásica/administração & dosagem , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Purging da Medula Óssea , Terapia Combinada , Ciclina D1/genética , Ciclofosfamida/administração & dosagem , Intervalo Livre de Doença , Doxorrubicina/administração & dosagem , Feminino , Seguimentos , Genes bcl-2 , Humanos , Cadeias Pesadas de Imunoglobulinas/genética , Leucaférese , Tábuas de Vida , Linfoma não Hodgkin/tratamento farmacológico , Linfoma não Hodgkin/mortalidade , Linfoma não Hodgkin/patologia , Linfoma não Hodgkin/terapia , Masculino , Pessoa de Meia-Idade , Neoplasia Residual , Reação em Cadeia da Polimerase , Valor Preditivo dos Testes , Prednisona/administração & dosagem , Proteínas Proto-Oncogênicas c-bcl-2/genética , Estudos Retrospectivos , Análise de Sobrevida , Resultado do Tratamento , Vincristina/administração & dosagem
14.
Bone Marrow Transplant ; 24(3): 345-8, 1999 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-10455379

RESUMO

Arsenic trioxide has recently been reported to be successful in the treatment of promyelocytic leukemia. Several concerns about the use of this toxic agent are currently reducing its potential clinical use even in severely ill patients. In this report we describe the results achieved by As2O3 with all-trans retinoic acid in a patient suffering from secondary, relapsed, resistant promyelocytic leukemia. Several complications, including sepsis and an extensive area of skin necrosis, did not allow us to treat the patient further with chemotherapy. With As2O3 and ATRA therapy, the patient obtained a complete molecular remission without any significant toxicity and, subsequently, it was possible to perform a bone marrow autograft in a state of complete remission.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Arsenicais/administração & dosagem , Leucemia Promielocítica Aguda/tratamento farmacológico , Segunda Neoplasia Primária/tratamento farmacológico , Óxidos/administração & dosagem , Tretinoína/administração & dosagem , Adulto , Trióxido de Arsênio , Transplante de Medula Óssea , Humanos , Masculino , Recidiva , Transplante Autólogo
16.
Cell Mol Biol (Noisy-le-grand) ; 44(1): 129-39, 1998 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-9551645

RESUMO

Chronic myeloid leukemia (CML), polycythemia vera (PV), idiopathic myelofibrosis (IM) and primary thrombocythemia (PT) are myeloproliferative diseases of clonal origin. Megakaryocyte series are commonly involved in these disorders. In a previous paper of us, megakaryocytes (MKs) from PV and PT patients were shown to be more pathological with respect to the MKs from CML. This paper describes a Fourier transform infrared microspectroscopy (FT-IR-M) study analyzing the cytoplasm and nucleus areas of MKs from thrombocythemic patients which exhibited numerous giant cells (from 100 to 190 microm in diameter). The size of these cells makes it possible to analyze the cell parts using FT-IR-M technique. The infrared determinations on 10 single MKs for each case examined in these two different cell regions revealed spectral differences with a high degree of reproducibility. Finally, the spectra of whole MKs from normal donors and from thrombocythemic patients were also compared.


Assuntos
Megacariócitos/citologia , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Trombocitose/sangue , Humanos
17.
Leukemia ; 12(3): 289-94, 1998 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-9529121

RESUMO

The efficacy of alfa-interferon (alfa-IFN) in essential thrombocythemia (ET) patients has been reported by several authors. The aim of this study is to assess the magnitude of the effect of alfa-IFN on the neoplastic clone. As of December 1993, 11 ET patients received alfa-IFN at a dose of 3-6 MU/s.c./day for 6 months. Ten of 11 obtained complete hematological remission (CHR) and one achieved partial hematological remission. Megakaryocyte concentration was reduced in six cases. The spleen,which was enlarged in four patients, decreased in size in two patients. Seven of eight patients who were symptomatic at diagnosis obtained resolution of symptoms. In order to obtain indications about the structural modifications induced by alfa-IFN in ET megakaryocytes (Mks), Fourier-transform infra-red microspectroscopy analysis performed on 10 single Mks of each patient, was done in seven of 11 patients; the analysis showed a reduction of A1/A2 ratios (A1 integrated area of the band at 1080 cm(-1) due to the nucleic acids absorption; A2 integrated area of the band at 1540 cm(-1) due to proteic components absorption) in five cases, and in three of these five patients A1/A2 ratios achieved normal values. After alfa-IFN treatment we did not observe any change in the methylation pattern of DNA from the granulocyte fraction. Our results confirm the efficacy of alfa-IFN in ET patients, and the decrease of A1/A2 ratios in several patients is a demonstration of the depth of the effect of alfa-IFN on the neoplastic clone. The results of clonality studies showed the persistence of clonal hematopoiesis. Whether higher alfa-IFN dose and/or more prolonged alfa-IFN therapy may allow a restoration of polyclonal hematopoiesis remains to be determined and should be explored in future clinical trials.


Assuntos
Interferon-alfa/uso terapêutico , Trombocitose/terapia , Adolescente , Adulto , Análise de Variância , DNA/sangue , Hematopoese , Células-Tronco Hematopoéticas/patologia , Heterozigoto , Humanos , Interferon-alfa/efeitos adversos , Contagem de Leucócitos , Megacariócitos/efeitos dos fármacos , Megacariócitos/patologia , Pessoa de Meia-Idade , Fosfoglicerato Quinase/genética , Contagem de Plaquetas , Polimorfismo de Fragmento de Restrição , Esplenomegalia/terapia , Trombocitose/sangue , Cromossomo X
18.
Bone Marrow Transplant ; 21(5): 519-20, 1998 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-9535045

RESUMO

We report a 21-year-old man who experienced symptoms of colitis following autologous TBI-conditioned PBSC transplantation, which persisted despite conventional treatment. Abdominal echography showed a thickened, stratified wall of the cecum, of the right colon and of part of the transverse colon. Hyperbaric oxygen therapy (20 sessions of 100% oxygen inhalation at 2.5 bar for 120 min in a hyperbaric chamber) achieved a prompt clinical recovery as well as complete disappearance of the ultrasound abnormalities.


Assuntos
Colite/etiologia , Colite/terapia , Oxigenoterapia Hiperbárica , Irradiação Corporal Total/efeitos adversos , Abdome/diagnóstico por imagem , Adulto , Colite/diagnóstico por imagem , Transplante de Células-Tronco Hematopoéticas , Humanos , Linfoma não Hodgkin/complicações , Linfoma não Hodgkin/radioterapia , Masculino , Recidiva , Condicionamento Pré-Transplante , Ultrassonografia
19.
Biopolymers ; 41(5): 545-53, 1997 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-9095677

RESUMO

The secondary structure of human fibrin from normal donors and from bovine and suilline plasma was studied by Fourier transform ir spectroscopy and a quantitative analysis of its secondary structure was suggested. For this purpose, a previously experimented spectrum deconvolution procedure based on the use of the Conjugate Gradient Minimisation Algorithm with the addition of suitable constraints was applied to the analysis of conformation-sensitive amide bands. This procedure was applied to amide I and III analysis of bovine and suilline fibrin, obtained industrially, and to amide III analysis of human fibrin clots. The analysis of both amide I and III in the first case was useful in order to test the reliability of the method. We found bovine, suilline, and human fibrin to contain about 30% alpha-helix (amide I and III components at 1653 cm-1, and 1312 and 1284 cm-1, respectively), 40% beta-sheets (amide I and III components at 1625 and 1231 cm-1, respectively) and 30% turns (amide I and III components at 1696, 1680, 1675 cm-1, and 1249 cm-1, respectively). The precision of the quantitative determination depends on the amount of these structures in the protein. Particularly, the coefficient of variation is < 10% for percentage values of amide I and III components > 15 and 5%, respectively. The good agreement of our quantitative data, obtained separately by amide I and amide III analysis, and consistent with a previous fibrinogen (from commercial sources) study that reports only information about fibrin beta-sheet content obtained by factor analysis, leads us to believe that the amounts of secondary structures found (alpha-helix, beta-sheets, and turns) are accurate.


Assuntos
Fibrina/química , Algoritmos , Animais , Bovinos , Humanos , Estrutura Molecular , Estrutura Secundária de Proteína , Espectroscopia de Infravermelho com Transformada de Fourier , Suínos
20.
Hematol Oncol ; 15(1): 27-31, 1997 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-9378469

RESUMO

The management of indolent lymphomas is still controversial. Intensive therapies may improve remission rate but in association with toxicity. Fludarabine and idarubicin are very active drugs in indolent lymphomas. This pilot trial was designed to evaluate the efficacy of a regimen comprising fludarabine, idarubicin and prednisone (FLIDA) in the treatment of low-grade non-Hodgkin's lymphoma at diagnosis. We have assessed the response of 16 adult patients (median age 57 years, range 45-71 years) treated on an outpatient basis: the overall response rate was 93.8 per cent (CR 43.8 per cent, PR > or = 50 per cent). The toxicity of this regimen was very low, with no relevant hematological and infectious complications.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Linfoma não Hodgkin/tratamento farmacológico , Idoso , Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos , Protocolos de Quimioterapia Combinada Antineoplásica/toxicidade , Feminino , Seguimentos , Humanos , Idarubicina/administração & dosagem , Idarubicina/toxicidade , Masculino , Pessoa de Meia-Idade , Projetos Piloto , Prednisona/administração & dosagem , Prednisona/toxicidade , Vidarabina/administração & dosagem , Vidarabina/análogos & derivados , Vidarabina/toxicidade
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