RESUMO
Common bean plants (Phaseolus vulgaris L.) showing different virus-like symptoms were collected in northwestern Argentina. Dot-blot hybridization tests showed that the begomoviruses bean golden mosaic virus and tomato yellow vein streak virus were the most prevalent, but they also revealed the presence of unknown begomoviruses. The complete genome sequence of one of these unknown begomoviruses was determined. Sequence analysis showed that the virus is a typical New World begomovirus, for which the name "bean bushy stunt virus" (BBSV) is proposed. Biological assays based on biolistic inoculations showed that BBSV induced leaf roll and stunting symptoms similar to those observed in the field-collected common bean sample.
Assuntos
Begomovirus/fisiologia , Phaseolus/virologia , Doenças das Plantas/virologia , Argentina , Sequência de Bases , Begomovirus/classificação , Begomovirus/genética , Begomovirus/patogenicidade , DNA Viral/genética , Genoma Viral/genética , Especificidade de Hospedeiro , Fases de Leitura Aberta , Phaseolus/crescimento & desenvolvimento , Filogenia , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/virologia , Glycine max/crescimento & desenvolvimento , Glycine max/virologiaRESUMO
We investigated the molecular characteristics of an Argentinean isolate of alfalfa leaf curl virus (ALCV-Arg), a virus of the genus Capulavirus in the family Geminiviridae that was isolated from alfalfa plants showing dwarfism. The genome was found to be 2,750 nucleotides in length. In pairwise comparisons, this ALCV isolate shared 83.2% to 92.6% sequence identity with European ALCV isolates. Sequence comparisons and phylogenetic analysis showed that this isolate combines features of strains A and B of ALCV. Recombination analysis showed that ALCV-Arg is a recombinant isolate that was generated by intraspecific recombination between ALCV strains A and B. The results of this study not only show that ALCV-Arg is unique because it combines features of strains A and B but also show that ALCV naturally infects this forage crop on the American continent.
Assuntos
DNA Viral/genética , Geminiviridae/genética , Genoma Viral , Medicago sativa/virologia , Filogenia , Folhas de Planta/virologia , Argentina , Sequência de Bases , Geminiviridae/classificação , Geminiviridae/isolamento & purificação , Sequenciamento de Nucleotídeos em Larga Escala , Doenças das Plantas/virologia , Recombinação Genética , Análise de Sequência de DNA , Homologia de Sequência do Ácido NucleicoRESUMO
Chia (Salvia hispanica), an herbaceous plant native to Latin America, has become important in the last 20 years due to its beneficial effects on health. Here, we present the first record and identification of two viruses in chia plants. The comparison of the complete nucleotide sequences showed the presence of two viral species with the typical genome organization of bipartite New World begomovirus, identified as Sida mosaic Bolivia virus 2 and Tomato yellow spot virus, according to the ICTV taxonomic criteria for begomovirus classification. DNA-A from Sida mosaic Bolivia virus 2 exhibited 96.1% nucleotide identity with a Bolivian isolate of Sida micrantha, and Tomato yellow spot virus showed 95.3% nucleotide identity with an Argentine bean isolate. This is the first report of begomoviruses infecting chia as well as of the occurrence of Sida mosaic Bolivia virus 2 in Argentina.
