Assuntos
Furagina/urina , Nitrofuranos/urina , Animais , Biofarmácia , Cápsulas , Nitrofurantoína/urina , Coelhos , Suspensões , Comprimidos , Fatores de TempoRESUMO
Methindion demethylation was shown to occur in rats. This process proceeds with the participation of NADPH-dependent transport system of microsomal electrons. In experiments in vivo methindion demethylation coursed at a high rate and was accompanied by a partial loss of its anticonvulsive effects.
Assuntos
Anticonvulsivantes/metabolismo , Indenos/metabolismo , Fígado/metabolismo , Animais , Transporte de Elétrons , Masculino , Microssomos Hepáticos/metabolismo , NADP/metabolismo , Ratos , Frações SubcelularesAssuntos
Anabolizantes/análise , Acetatos , Alcanos , Cromatografia em Camada Fina , Etil-Éteres , Lipídeos/análise , Métodos , SolventesRESUMO
Three chemical methods for determination diethylstilbestrol (DES) in biological material (Jons and Deferage, 1953; Ponder, 1966; Official Method of Pharmacopoeia, USA, 1965) were compared. The most adequate method for determination of DES in animal tissue is the Official method of Pharmacopoeia based on a specific reaction. The method yields reproducible results. Average recovery of pure DES added to biological material was 78%. The method by Jons and Deferage though providing recovery of 86% of the added DES is based on nonspecific reaction with SbCl5, which resulted in too high results. Method of Pharmacopoeia (USA) was used to determine residual amounts of DES in tissues of a rabbit, implanted with 0.1 mg DES per 1 kg weight. One month after implantation, trace amounts of DES were detected only in muscle tissues. Two and three months after implantation, the residual amounts of DES in the rabbit tissues were not detected.