In Vivo Animal Study of a Highly Viscous N-butyl Cyanoacrylate Medical Adhesive for Intravenous Embolization.

N-butyl cyanoacrylate (NBCA) is a liquid monomer that undergoes an exothermic polymerization reaction to form a solid upon initiation with hydroxyl anions. Recently, EGpresto, a highly viscous NBCA-based adhesive, has been developed for vascular-occlusion purposes. In this study, we investigated the heat of polymerization of EGpresto and compared the results with those of a low-viscosity NBCA glue. Results show that EGpresto exhibited a lower heat of polymerization (64 ± 7 °C vs. 34 ± 1 °C). This was due to its high viscosity, which resulted in a delayed polymerization time. To investigate the efficacy and safety of EGpresto for intravenous embolization, a 14 d in vivo animal test was conducted using three pigs. Five cc of EGpresto was injected into the epigastric vein of each animal. Complete postoperative vein occlusion was confirmed at 7 and 14 d by ultrasonographic visualization. After the animals were sacrificed, the operated and unoperated veins were exposed, and the injected adhesive was found without migration. During the histology, the injected adhesive was not found in the inner or outer vein walls, and the immune reactions seemed to be the only foreign-body reaction, showing that EGpresto is a non-toxic and safe intravascular embolic agent.

Fabrication of inverse opal photonic gel sensors on flexible substrates by transfer process.

We demonstrate a platform technology for transferring opal films and photonic gel films to flexible substrates. The conventional fabrication procedure for inverse opal photonic gel (IOPG) sensors comprises three major steps: 1) the self-assembly of polystyrene µ-spheres to an opal template film within a channel between the top and bottom substrates, 2) infiltration and photo-polymerisation of the monomer mixture, and 3) etching of the opal template. Owing to the low processing yield of the first step, it is difficult to fabricate multiple sensor arrays on a single substrate. In this study, an opal film is formed between two substrates with different surface polarities, and the film is separated by disassembling the two substrates. The opal film on a medium polar substrate is covered using a flexible polyethylene terephthalate (PET) film, and opal-templated photo-polymerisation is performed. Finally, the photonic gel with the opal template is transferred to the PET film, and the opal template is etched out. Using the platform technique, the fabrications of pH-responsive IOPG and temperature-responsive IOPG sensors on PET films are respectively demonstrated. In addition, the IOPG containing the copolymer of acrylamide and 3-acrylamidophenylboronic acid was found to be responsive to glucose at physiological pH. All three sensors were fabricated using the same transfer method, differing only in the composition of monomer mixtures, and they all showed excellent sensitivity and repeatability on PET substrates. Due to the advantageous feature of the transfer method, dual sensors of pH-responsive IOPG and temperature-responsive IOPG were sequentially fabricated on a single PET film.