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This study investigated the biocompatibility of strontium-doped calcium phosphate (Sr-CaP) coatings on pure magnesium (Mg) surfaces for bone applications. Sr-CaP coated specimens were obtained by chemical immersion method on biodegradable magnesium. In this study, Sr-CaP coated magnesium was obtained by immersing pure magnesium in a solution containing Sr-CaP at 80 °C for 3 h. The corrosion resistance and biocompatibility of magnesium according to the content of Sr-CaP coated on the magnesium surface were evaluated. As a result, the corrosion resistance of Sr-CaP coated magnesium was improved compared to pure magnesium. In addition, it was confirmed that the biocompatibility of the group containing Sr was increased. Thus, the Ca-SrP coating with a reduced degradation and improved biocompatibility could be used in Mg-based orthopedic implant applications.
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Acute liver injury (ALI) is a life-threatening clinical syndrome. Long-lasting liver injury can lead to chronic hepatic inflammation and fibrogenic responses. Zerumbone (ZER), the main constituent of rhizomes of Zingiber zerumbet Smith, has a variety of functions including anticancer activity. We investigated the role of ZER on the progression of hepatotoxin-induced liver injury. Single or repeated injection of CCl4 was used to induce acute or chronic liver injury, respectively. Mice were orally administered with ZER (10, 50 mg/kg) during the experimental period. Histopathologic analysis and serum biochemical levels revealed that ZER had hepatoprotective activities against ALI. Similar effects of ZER on injured livers were confirmed by analyses of inflammation and apoptosis-related genes. Western blot analysis showed that protein levels of apoptotic molecules were decreased, whereas antiapoptotic protein levels were conversely increased in injured livers treated with ZER. Furthermore, chronic liver injury and its associated fibrogenesis in mice were reduced by ZER treatment. These findings from our in vivo experiments further indicate that ZER could alleviate hepatocellular toxicity and inhibit activation of primary hepatic stellate cells. Our results suggest that ZER might have potential as a safe and prophylactic alternative to prevent acute and chronic liver injury.
Assuntos
Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Fígado/efeitos dos fármacos , Sesquiterpenos/uso terapêutico , Doença Aguda , Animais , Apoptose , Doença Hepática Induzida por Substâncias e Drogas/patologia , Doença Crônica , Fígado/patologia , Masculino , Camundongos , Sesquiterpenos/química , Sesquiterpenos/farmacologiaRESUMO
Osseointegration of dental implants is affected by osteoporosis. The purpose of this study was overcome the implant failure and facilitate the osseointegration of dental implants by c-myb in ovariectomized (OVX)-induced osteoporosis. c-myb is a transcription factor and supports bone formation. Plasmid DNA/c-myb conjugated with chitosan-gold nanoparticles (Ch-GNPs/c-myb) promoted osteogenesis and inhibited osteoclastogenesis in MC-3T3 E1 cells. Ch-GNPs/c-myb involved the reduction of the nuclear factor of activated T-cells 1, c-Fos, and tartrate-resistant acid phosphatase-positive multinucleated osteoclasts in receptor activator of nuclear factor-κB ligand (RANKL) stimulated bone marrow macrophages. In vivo results of rat mandibles demonstrated Ch-GNP/c-myb-coated titanium (Ti) implants increased the volume and density of newly formed bone and the osseointegration of dental implant with bone by micro computed tomography examination after OVX-induced osteoporosis. Immunohistochemical analysis showed increased c-myb expression and upregulation of bone morphogenic proteins, osteoprotegerin and EphB4, as well as the downregulation of RANKL by Ch-GNP/c-myb-coated Ti implants. Hematoxylin and Eosin staining expressed new bone formation by Ch-GNP/c-myb-coated Ti implants. Our findings indicated that c-myb delivered by Ch-GNPs supports osseointegration of dental implant even in osteoporotic condition. c-myb may be applicable to support dental implant integration and treatment in age-dependent bone destruction disease.
Assuntos
Quitosana , Implantes Dentários , Técnicas de Transferência de Genes , Ouro , Nanopartículas Metálicas , Osseointegração , Proteínas Proto-Oncogênicas c-myb , Animais , Linhagem Celular , Quitosana/química , Quitosana/farmacologia , Feminino , Ouro/química , Ouro/farmacologia , Nanopartículas Metálicas/química , Nanopartículas Metálicas/uso terapêutico , Ovariectomia , Proteínas Proto-Oncogênicas c-myb/biossíntese , Proteínas Proto-Oncogênicas c-myb/genética , Ratos , Ratos Sprague-DawleyRESUMO
Poor bone quality and osteolysis are the major causes of implant failure in dentistry. Here, this study tested the effect of phelligridin D-loaded nanotubes titanium (Ti) for bone formation around the dental implants. The purpose of this study was to enhance osseointegration of phelligridin D-loaded implant into the bone for bone formation and prevention of osteolysis. Cell viability, crystal violet staining, Western blot, alizarin red S staining, alkaline phosphatase activity, tartrate-resistant acid phosphatase staining, micro-computed tromography (µ-CT), hematoxylin and eosin (H&E) and immunohistochemical staining were used in vitro and in vivo to test the biocompatibility of phelligridin D. Phelligridin D enhanced osteoblast differentiation and mineralization by increasing bone morphogenic protein-2/7 (BMP-2/7), Osterix, Runx-2, osteoprotegerin (OPG), alkaline phosphatase and inhibited osteoclast differentiation by decreasing receptor activator of nuclear factor kappa-B ligand (RANKL) in MC-3T3 E1 cells. Further, phelligridin D promoted bone regeneration around nanotube Ti implant surface by increasing the levels of BMP-2/7 and OPG in a rat model. Phelligridin D also inhibited osteolysis by suppressing the expression of RANKL. These findings strongly suggest that phelligridin D is a new compound representing a potential therapeutic candidate for implant failure caused by osteolysis and poor bone quality of teeth.
Assuntos
Benzopiranos/farmacologia , Portadores de Fármacos/química , Mandíbula/efeitos dos fármacos , Mandíbula/fisiologia , Nanotubos/química , Osseointegração/efeitos dos fármacos , Osteólise/prevenção & controle , Titânio/química , Células 3T3 , Administração Oral , Animais , Benzopiranos/administração & dosagem , Benzopiranos/química , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células , Sobrevivência Celular/efeitos dos fármacos , Portadores de Fármacos/administração & dosagem , Liberação Controlada de Fármacos , Masculino , Mandíbula/patologia , Camundongos , Osteoblastos/citologia , Osteoblastos/efeitos dos fármacos , Osteogênese/efeitos dos fármacos , Osteólise/metabolismo , Osteólise/patologia , Próteses e Implantes , Ratos , Ratos Sprague-Dawley , Titânio/administração & dosagemRESUMO
Diabetes mellitus (DM) has a detrimental effect on osseointegration, stability and longevity of implants due to osteoporosis. In this study, PPARγ-loaded dental implants were investigated for the improvement of osseointegration and peri-implantitis. Chitosan gold nanoparticles conjugated with PPARγ cDNA were introduced on titanium mini-implant surfaces for PPARγ release to rat mandibular. DM-induced rat mandible showed structural changes such as decreased bone mass and increased inflammatory molecules, and diminution of PPARγ expression and bone formation molecules compared to normal rats. PPARγ induced bone formation via reduction of inflammatory molecules even under glucose oxidative stress. Furthermore, PPARγ strongly activated mitochondrial biogenesis and cell viability via p-AMK and Wnt/ß-catenin signaling. Consequently, PPARγ gene delivery on regional dental implants contributed osseointegration, new bone formation and mineralization in DM-induced rats. This study demonstrates that PPARγ can be used as a therapeutic gene with dental implantation in diabetic patients since regional PPARγ expression enhances osseointegration and implant longevity.
Assuntos
Implantes Dentários , Diabetes Mellitus , Técnicas de Transferência de Genes , Nanopartículas , Osseointegração , PPAR gama/genética , Animais , Desenvolvimento Ósseo , Mandíbula , Biogênese de Organelas , Osteoporose/complicações , Ratos , TitânioRESUMO
Surface modification to improve the corrosion resistance and biocompatibility of the Mg-Al-Zn-Ca alloy was conducted via plasma electrolytic oxidation (PEO) in an electrolyte that included phosphate. Calcium phosphate can be easily induced on the surface of a PEO coating that includes phosphate in a physiological environment because Ca(2+) ions in body fluids can be combined with PO4 (3-). Cytotoxicity of the PEO coating formed in electrolytes with various amounts of Na3PO4 was identified. In particular, the effects that PEO films have upon oxidative stress and differentiation of osteoblast activity were studied. As the concentration of Na3PO4 in the electrolyte increased, the oxide layer was found to become thicker, which increased corrosion resistance. However, the PEO coating formed in electrolytes with over 0.2 M of added Na3PO4 exhibited more microcracks and larger pores than those formed in smaller Na3PO4 concentrations owing to a large spark discharge. A nonuniform oxide film that included more phosphate caused more cytotoxicity and oxidative stress, and overabundant phosphate content in the oxide layer interrupted the differentiation of osteoblasts. The corrosion resistance of the magnesium alloy and the thickness of the oxide layer were increased by the addition of Na3PO4 in the electrolyte for PEO treatment. However, excessive phosphate content in the oxide layer led to oxidative stress, which resulted in reduced cell viability and activity.
Assuntos
Ligas/química , Materiais Biocompatíveis/química , Eletrólitos/metabolismo , Osteoblastos/fisiologia , Fosfatos/metabolismo , Plasma/química , Propriedades de Superfície/efeitos dos fármacos , Diferenciação Celular , Oxirredução , Estresse OxidativoRESUMO
PURPOSE: The histone deacetylase (HDAC) inhibitor suberoylanilide hydroxamic acid (SAHA) has been reported to exhibit anticancer activities in various cancer cell types, but as yet there are few reports on the anticancer effects of SAHA in oral squamous cell carcinoma (OSCC)-derived cells and xenograft models. METHODS: The anti-proliferative and apoptotic activities of SAHA were assessed in human HSC-3 and HSC-4 (OSCC)-derived cell lines and JB6 normal mouse skin-derived epidermal cells using histone acetylation, soft agar colony formation, trypan blue exclusion, 4'-6-diamidino-2-phenylindole (DAPI) staining, Live/Dead viability/cytotoxicity and Western blot analyses. RESULTS: We found that SAHA treatment resulted in hyperacetylation of histones H2A and H3 and a concomitant decrease in the viability of HSC-3 and HSC-4 cells. SAHA also significantly inhibited the neoplastic transformation of JB6 cells treated with TPA, whereas the viability of these cells was not affected by this treatment. Additionally, we found that SAHA suppressed the anchorage-independent growth (colony forming capacity in soft agar) of HSC-3 and HSC-4 cells. DAPI staining, Live/Dead and Western blot analyses revealed that SAHA can induce caspase-dependent apoptosis in HSC-3 and HSC-4 cells. We also found that SAHA treatment led to inhibition of ERK phosphorylation, and that two MEK inhibitors potentiated SAHA-mediated apoptosis. Okadaic acid treatment inhibited SAHA-mediated apoptosis in both the HSC-3 and HSC-4 cell lines, wheras SAHA induced a profound in vivo inhibition of tumor growth in HSC-3 xenografts. CONCLUSIONS: Our results indicate that the ERK signaling pathway may constitute a critical denominator of SAHA-induced apoptosis in OSCC-derived cells and that SAHA may have therapeutic potential for OSCC.
Assuntos
Ácidos Hidroxâmicos/farmacologia , Neoplasias Bucais/patologia , Acetilação/efeitos dos fármacos , Animais , Apoptose/efeitos dos fármacos , Caspases/metabolismo , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Histonas/metabolismo , Humanos , Masculino , Camundongos , Neoplasias Bucais/enzimologia , Ácido Okadáico/farmacologia , Fosforilação/efeitos dos fármacos , Inibidores de Proteínas Quinases/farmacologia , Ensaio Tumoral de Célula-Tronco , Vorinostat , Ensaios Antitumorais Modelo de XenoenxertoRESUMO
Insulin like growth factor binding protein-3 (IGFBP-3) in bone cells and its utilization in dental implants have not been well studied. The aim of this study was to determine the osteogenic efficacy of chitosan gold nanoparticles (Ch-GNPs) conjugated with IGFBP-3 coated titanium (Ti) implants. Ch-GNPs were conjugated with IGFBP-3 plasmid DNA through a coacervation process. Conjugation was cast over Ti surfaces, and cells were seeded on coated surfaces. For in vitro analysis the expression of different proteins was analyzed by immunoblotting. For in vivo analysis, Ch-GNP/IGFBP-3 coated implants were installed in rat mandibles. Four weeks post-implantation, mandibles were examined by microcomputed tomography (µCT), immunohistochemistry, hematoxylin & eosin and tartrate resistance acid phosphatase staining. In vitro overexpressed Ch-GNP/IGFBP-3 coated Ti surfaces was associated with activation of extracellular signal related kinase (ERK), inhibition of the stress activated protein c-Jun N-terminal kinase (JNK) and enhanced bone morphogenetic protein (BMP)-2 and 7 compared to control. Further, in vivo, Ch-GNP/IGFBP-3 coated implants were associated with inhibition of implant induced osteoclastogenesis molecules, receptor activator of nuclear factor kappa-B ligand (RANKL) and enhanced expression of osteogenic molecules including BMP2/7 and osteopontin (OPN). The µCT analysis demonstrated that IGFBP-3 increased the volume of newly formed bone surrounding the implants compared to control (n=5; p<0.05). These results support the view that IGFBP-3 overexpression diminishes osteoclastogenesis and enhances osteogenesis of Ti implants, and can serve as a potent molecule for the development of good implantation.
Assuntos
Implantes Dentários , Proteína 3 de Ligação a Fator de Crescimento Semelhante à Insulina/fisiologia , Mandíbula/cirurgia , Osteogênese , Células 3T3 , Animais , Masculino , Camundongos , Ratos , Ratos Sprague-DawleyRESUMO
This study investigated the effects of anodization-cyclic precalcification-heat (APH) treatment on the bonding ability of Ca-P coating to the parent metal and osseointegration of Ti-6Al-7Nb implants. Eighteen Ti-6Al-7Nb discs, 9 untreated and 9 APH-treated, were cultured with osteoblast cells in vitro, and the cellular differentiation ability was assayed at 1, 2, and 3 weeks. For in vivo testing, 28 Ti-6Al-7Nb implants (14 implants of each group) were inserted to rat tibias, and after each 4 and 6 weeks of implantation, bone bonding, and osseointegration were evaluated through removal torque and histological analysis. Osteoblast-culturing showed twice as much of the alkaline phosphatase activity on the treated surface at 3 weeks than on the untreated surface (p < 0.05). The treated implants exhibited higher removal torque values than the untreated ones (15.5 vs. 1.8 Ncm at 4 weeks and 19.7 vs. 2.6 Ncm at 6 weeks, p < 0.05). Moreover, the excellent bonding quality of coats was confirmed by the existence of cohesive fractures on the surface of removed APH implants (field emission scanning electron microscopy and histological observation). Within the limits of this study, it can be concluded that the APH treatment significantly enhanced osseointegration of the Ti-6Al-7Nb implant, with the stable bonding between the coating and the implant surface.
Assuntos
Materiais Revestidos Biocompatíveis/farmacologia , Ligas Dentárias/farmacologia , Implantes Dentários , Osseointegração , Óxidos/farmacologia , Titânio/farmacologia , Fosfatase Alcalina/análise , Animais , Células Cultivadas , Temperatura Alta , Masculino , Teste de Materiais , Osteoblastos/efeitos dos fármacos , Oxirredução , Período Pós-Operatório , Ratos , Ratos Sprague-Dawley , Propriedades de Superfície , Tíbia/metabolismo , Tíbia/patologia , Tíbia/cirurgia , TorqueRESUMO
Titanium (Ti) is often used as an orthopedic and dental implant material due to its better mechanical properties, corrosion resistance, and excellent biocompatibility. Formation of TiO2 nanotubes (TiO2 NTs) on titanium is an interesting surface modification to achieve controlled drug delivery and to promote cell growth. Carbon nanotubes (CNTs) possess excellent chemical durability and mechanical strength. The use of CNTs in biomedical applications such as scaffolds has received considerable attention in recent years. The present study aims to modify the surface of titanium by anodizing to form TiO2 NTs and subsequently deposit CNTs over it by electrophoretic deposition (EPD). Characteristic, biocompatibility, and apatite forming ability of the surface modified samples were evaluated. The results of the study reveal that CNTs coating on TiO2 nanotubes help improve the biological activity and this type of surface modification is highly suitable for biomedical applications.
RESUMO
The methodology of deposition of fluoride conversion coatings is modified with the use of galvanic coupling, agitation of the electrolyte solution, and addition of K2CO3, which helps to provide a better understanding of the mechanism and new avenues to tailor the composition of the coating. A very good correlation exists between the F/O ratio of the coatings prepared under varying experimental conditions and their icorr, |Z| and phase angle maximum; the higher the F/O ratio, the better the corrosion protective ability of the coatings in Hank's balanced salt solution. The corrosion behaviour of the coatings of the present study suggests that fluoride conversion coatings show much promise for their use for biomedical applications, as long as their uniformity is improved and the composition is tailored to enrich the MgF2 phase, encompassing a higher F/O ratio.
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The warming acupuncture for hyperthermia therapy is made of STS304. However, its needle point cannot be reached to a desirable temperature due to heat loss caused by low thermal conductivity, and the quantification of stimulation condition and the effective standard establishment of warming acupuncture are required as a heat source. Accordingly, in this study, after Ag-Cu alloys with different composition ratios were casted and then mixed with additives to improve their physical and mechanical properties, the thermal conductivity and biocompatibility of the alloy specimens were evaluated for selecting suitable material. Ag-Cu binary alloys and ternary alloys added 5 wt% Zn or 2 wt% Mg were casted and then cold drawn to manufacture needles for acupuncture, and their physical properties, thermal conductivity, and biocompatibility were evaluated for their potential use in warming acupuncture. The results of this study showed that the physical and mechanical properties of the Ag-Cu alloys were improved by additives and that the thermal conductivity, machinability, and biocompatibility of the Ag-Cu alloys were improved by Mg addition.
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New strategies involving drugs loading onto implant surfaces are required to enhance osseointegration and shorten healing time after implantation. In this study, we examined the feasibility of N-acetyl cysteine (NAC)-loaded nanotube titanium (NLN-Ti) implants as a potential drug delivery system. To determine the effect of NLN-Ti in in vitro and in vivo, viability and ROS formation was assessed and enzyme-linked immunosorbant assay (ELISA), Western blot, micro-computed tomography (µ-CT), hematoxylin and eoxin (H&E) staining and immunohistochemical (IHC) analysis were done. In vitro, cell viability was increased and inflammatory responses and reduced oxidative stress-related defense were decreased with MC 3T3-E1 cells exposed to a sustained release of NAC from NLN-Ti implants. Following NLN-Ti implant installation, µ-CT revealed an increase of newly formed bone volume and bone mineral density in the mandibles of Sprague Dawley rats. Relatively well formed new bone was demonstrated in close contact to the NLN-Ti implant surface by H&E staining. IHC revealed significantly higher expression of bone morphogenetic protein-2, -7 and heme oxygenase-1, and reduced expression of receptor activator of nuclear factor-kappa B ligand. The data indicate that NLN-Ti implants enhance osseointegration and highlight the value of the small animal model in assessing diverse biological responses to dental implants.
Assuntos
Regeneração Óssea/fisiologia , Cisteína/química , Implantes Dentários , Nanotubos/química , Titânio/química , Animais , Western Blotting , Linhagem Celular , Sobrevivência Celular/fisiologia , Mandíbula/cirurgia , Camundongos , Ratos Sprague-Dawley , Espécies Reativas de Oxigênio/metabolismoRESUMO
The objective of this study is to investigate the effect of cyclic precalcification treatment to impart bioactive properties for titanium implants. Before precalcification, the titanium implants were subjected to blasting using hydroxyapatite (HAp), a resorbable blasting medium (RBM treated), and anodized using an electrolyte containing glycerol, H2O, and NH4F. Precalcification treatment was performed by two different methods, namely, continuous immersion treatment (CIT) and alternate immersion treatment (AIT). In CIT, the RBM treated and anodized titanium implants were immersed in 0.05 M NaH2PO4 solution at 80°C and saturated Ca(OH)2 solution at 100°C for 20 min, whereas during AIT, they were immersed alternatively in both solutions for 1 min for 20 cycles. Anodizing of the titanium implants enables the formation of self-organized TiO2 nanotubes. Cyclic precalcification treatment imparts a better bioactive property and enables an increase in activation level of the titanium implants. The removal torque values of the RBM treated, CIT treated, and AIT treated titanium implants are 10.8 ± 3.7 Ncm, 17.5 ± 3.5 Ncm, and 28.1 ± 2.4 Ncm, respectively. The findings of the study indicate the cyclic precalcification in an effective surface treatment method that would help accelerate osseointegration and impart bioactive property of titanium implants.
Assuntos
Materiais Biocompatíveis/farmacologia , Fosfatos de Cálcio/farmacologia , Teste de Materiais/métodos , Nanotubos/química , Próteses e Implantes , Titânio/farmacologia , Animais , Apatitas/química , Eletrodos , Masculino , Nanotubos/ultraestrutura , Implantação de Prótese , Ratos , Ratos Wistar , Espectrometria por Raios X , Propriedades de Superfície , Torque , Microtomografia por Raio-XRESUMO
OBJECTIVES: To deliver the efficacy and safety of Ch-GNPs (Chitosan gold nanoparticles) conjugated anti-inflammatory molecules peroxisome proliferator activated receptor gamma (PPARγ) on implant surface titanium (Ti) to reduce implant-induced inflammation. MATERIALS AND METHODS: The Ch-GNPs were conjugated with the PPARγ cDNA through a coacervation process. Conjugation was cast over Ti surfaces by dipping, and cells were seeded on different sizes (6 × 6 × 0.1 cm and 1 × 1 × 0.1 cm; n = 3) of Ti surfaces. The size of Ch-GNPs and surface characterization of Ti was performed using UV-vis spectroscopy, TEM (Transmission electron microscopy) and EDX (energy-dispersive X-ray). The DNA conjugation and transfection capacity of Ch-GNPs were simultaneously confirmed by agarose gel electrophoresis, ß-galactosidase staining, and immunoblotting. RESULTS: The Ch-GNPs were well dispersed and spherical in shape, with average size around 10-20 nm. Ti surfaces coated with Ch-GNPs/LacZ, as transfection efficacy molecule, showed strong ß-galactosidase staining in MC-3T3 E1 cells. Cells cultured on Ch-GNPs/PPARγ-coated Ti surfaces were able to inhibit implant-induced inflammation by simultaneously suppressing the expression of tumor necrosis factor- alpha (TNF-α), interleukin-1 beta (IL-1ß), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and matrix metalloproteinase-2 (MMP-2). The inhibition mechanism of Ch-GNPs/PPARγ was due to inhibition of both reactive oxygen species (ROS) and nitric oxide (NO) secretion (n = 3; P < 0.05). In addition, Ch-GNPs/PPARγ was able to increase expression of bone morphogenetic protein (BMP-7) and runt-related transcription factor-2 (RUNX-2). Furthermore, alkaline phosphatase activity (ALP) was also increased than that in control (n = 3; P < 0.01). Whereas, expression of receptor activator of NF-κB ligand (RANKL) was decreased. CONCLUSIONS: The novel gene delivery materials, like Ch-GNPs, can carry the PPARγ cDNA into the required areas of the implant surfaces, thus aiding to inhibit inflammation and promote osteoblast function. Thus, the PPARγ on implant surfaces may promote its clinical application on peri-implantitis or periodontitis like diseases.
Assuntos
Células 3T3/efeitos dos fármacos , Calcificação Fisiológica/efeitos dos fármacos , Quitosana/farmacologia , Ouro/farmacologia , Osteoblastos/efeitos dos fármacos , PPAR gama/farmacologia , Peri-Implantite/prevenção & controle , Células 3T3/metabolismo , Fosfatase Alcalina/metabolismo , Animais , Eletroforese em Gel de Ágar , Camundongos , Nanopartículas , Óxido Nítrico/metabolismo , Osteoblastos/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Espectrofotometria Ultravioleta , Coloração e Rotulagem , Propriedades de Superfície , Titânio/química , TransfecçãoRESUMO
Nanostructure surface of titanium implants treated with anodic oxidation, heat, and bisphosphonates, has been introduced to improve osseointegration of the implants. However, no information could be found about the efficiency of these approaches on Ti-6Al-4V alloy surfaces. This study examined the drug loading capacity of anodized nanotubular Ti-6Al-4V alloy surfaces in vitro as well as the bone response to surface immobilized bisphosphonates (BPs) on anodized nanotubular Ti-6Al-4V alloy surface in tibiae of rats. Ti-6Al-4V alloy titanium was divided into two groups: (1) control group (nontreated); (2) test group (anodized, heat-, and bisphosphonate-treated group). In vitro, amount of the drug released from the both groups' specimens was examined; all samples were 1 × 2 cm in size. In vivo, the 10 implants were placed inside of tibias of five rats. After 4 weeks, the bone response of the implants was evaluated using a removal torque test, and measuring bone contact and bone area. In addition, the surfaces of the extracted implants were observed by FE-SEM and EDS. In vitro, the drug loading capacity of the Ti-6Al-4V alloy surfaces was enhanced by anodizing surface modification. The values of the removal torque, bone contact, and bone area were significantly higher in the test group (p < 0.05). Furthermore, according to the EDS analysis, the amounts of Ca and P on the surface of the extracted implants were higher in the test group. Within the limits of this experiment, results of this research demonstrated that bisphosphonate-treated Ti-6Al-4V alloy implants with nanotubular surfaces have positive effects in bone-to-implant contact.
Assuntos
Conservadores da Densidade Óssea , Substitutos Ósseos , Difosfonatos , Teste de Materiais , Nanotubos/química , Fraturas da Tíbia/terapia , Titânio , Ligas , Animais , Conservadores da Densidade Óssea/química , Conservadores da Densidade Óssea/farmacologia , Substitutos Ósseos/química , Substitutos Ósseos/farmacologia , Difosfonatos/química , Difosfonatos/farmacologia , Ácido Ibandrônico , Masculino , Porosidade , Ratos , Ratos Wistar , Fraturas da Tíbia/patologia , Titânio/química , Titânio/farmacologiaRESUMO
This study was conducted to investigate the biocompatibility of Mg-Zn-Ca ternary alloy as a biodegradable material. The casting alloy underwent anodization in an alkaline electrolyte at current density 300 mA/cm(2) and frequency 50 Hz to obtain porous oxide layer. Plasma anodization film using pulse was shown to form irregular porous oxide film. As a result of corrosion test, the corrosion current was shown to decrease and the corrosion voltage was shown to increase in the anodized group, which showed the improvement of corrosion resistance after surface treatment. Sodium silicate (0.1 M) was directly oxidized due to high charges caused by spark and then formed SiO(2), and the compounds produced inside the film were shown MgO, Mg(2) SiO(4), and SiO(2.) In the histological examination in rats, all samples of the untreated group were shown to be absorbed 3 weeks later into the body. After the magnesium alloy was implanted, blood vessel expansion and tissue change were shown in the adjacent tissues. However, the changed tissues were shown to return to normal muscle tissues 4 weeks later when the alloy was completely absorbed. These results suggest that anodized Mg-35Zn-3Ca alloy has good biocompatibility in vivo and controls the absorption rate of biomaterials.
Assuntos
Implantes Absorvíveis , Ligas/química , Cálcio/química , Magnésio/química , Zinco/química , Células 3T3 , Absorção , Animais , Materiais Biocompatíveis/química , Vasos Sanguíneos/metabolismo , Corrosão , Masculino , Teste de Materiais , Metais/química , Camundongos , Oxigênio/química , Ratos , Ratos Sprague-Dawley , Propriedades de SuperfícieRESUMO
The dispersing power of surfactant-modified multiwalled carbon nanotubes (MWCNTs) and their effect on the antibacterial activity were examined. The MWCNTs were modified using a dioctyl sodium sulfosuccinate (AOT) surfactant. UV-vis spectroscopy and transmission electron microscopy (TEM) were used to characterize the dispersion of MWCNTs in the aqueous phase. Fourier transform infrared spectroscopy confirmed the results of UV-vis spectroscopy and TEM, indicating that the AOT molecules had been adsorbed successfully onto the MWCNT surface. The highly dispersed AOT-modified MWCNTs showed strong antibacterial activity to Streptococcus mutans. The fluorescence images showed that the AOT-modified MWCNTs were capable of capturing bacteria and forming cell aggregates as well as killing them. The optical density growth curves and colony-forming units assays confirmed that the antibacterial activity of the AOT-modified MWCNTs was concentration-dependent and treatment time-dependent. This finding might be useful for applications of AOT-modified MWCNTs as an antibacterial agent to eliminate pathogens from a biocontaminated water phase.
Assuntos
Antibacterianos/farmacologia , Nanotubos de Carbono , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Espectrofotometria Ultravioleta , Espectroscopia de Infravermelho com Transformada de Fourier , Streptococcus mutans/efeitos dos fármacosRESUMO
Bioactive ceramic coatings on titanium substrates were prepared successfully by micro-arc oxidation coupled with electrophoretic deposition (MAO and EPD) in NaOH electrolyte solution containing hydroxyapatite (HA) particles. The HA suspensions with various NaOH concentrations were prepared by ultrasonic dispersion. The microstructure, as well as the elemental and phase composition of the coatings was examined by scanning electron microscopy, energy dispersive spectrometry, and X-ray diffraction. X-ray diffraction showed that the coatings were composed mainly of rutile, Na(2)Ti(6)O(13), and HA phases. The composition and surface morphologies are strongly dependent on the NaOH electrolyte concentration. The corrosion behavior of the coating layers in simulated body fluids was evaluated using a potentiodynamic polarization test. The corrosion resistance of the coated sample was increased compared with the untreated titanium sample. The in vitro bioactivity assessment showed that the MAO and EPD-treated titanium substrate possesses higher apatite-forming ability than the only MAO-treated titanium substrate. In addition, the cell behavior was also examined using cell proliferation assay, cell morphology, and alkaline phosphatase activity. They obtained an agreement with the result of apatite-forming ability. The results clearly show that combining the MAO and EPD techniques provides an optimal surface for cell differentiation and osseointegration.
Assuntos
Cerâmica , Durapatita , Eletroforese/métodos , Titânio , Células 3T3 , Animais , Camundongos , Oxirredução , Análise Espectral/métodos , Difração de Raios XRESUMO
TiO(2) nanotube array (TN) on titanium plate was fabricated by using an electrochemical method. The crystal structure and surface morphology of TN array was examined by X-ray diffraction (XRD) and Field Emission Scanning Electronic Microscopy (FE-SEM), respectively. The stability of the nanotube structure and crystal phase transition was studied at different temperatures in dry oxygen ambient. The as-deposited films were found to be amorphous. The tubes crystallized in the anatase phase at a temperature of 450 degrees C. Anatase crystallites formed inside the tubes walls was transformed completely to rutile at 500 degrees C in dry environment. With the heating temperature increased the intensity of rutile peak increased with decrease in reflection from titanium. Intense rutile peak was observed at 600 degrees C. The average pore diameter as calculated from FE-SEM images was 50-100 nm. At higher temperature tubular structure completely collapsed leaving dense rutile crystallites. A model was proposed to explain the formation mechanism of TN fabricated on titanium plate in HF/H(2)SO(4) electrolyte.