Laboratory and Field Evaluation of Host-Related Foraging Odor-Cue Combinations to Attract Drosophila suzukii (Diptera: Drosophilidae).

The invasive spotted-wing drosophila, Drosophila suzukii (Matsumura), is a major pest of soft-skinned fruits. Since its introduction into North America and Europe, significant progress has been made in understanding the volatile cues used by this fly during food, oviposition site, and mate finding. Despite this progress, commercially available lures are non-selective. Here, we tested two Hanseniaspora uvarum (Niehaus) yeast compounds (isoamyl acetate and isobutyl acetate) and a leaf compound ß-cyclocitral alone and in combination with a blend of four fermentation compounds ('Fermentation lure': acetic acid, ethanol, methionol, and acetoin) to improve D. suzukii attraction and selectivity. In laboratory assays, males and females were attracted to all seven individual compounds, although in electrophysiological assays, their antennae exhibited a dose-dependent response to only four of these compounds. In two-choice cage studies, the Fermentation lure was more attractive to D. suzukii than water controls, whereas ß-cyclocitral and the mixture of isoamyl acetate and isobutyl acetate were not attractive in this larger-cage study. Moreover, adding the two-component H. uvarum compound blend to the Fermentation lure reduced D. suzukii attraction to the Fermentation blend. When these experiments were repeated in blueberry, raspberry, blackberry, and cherry orchards across several states in the United States over 2 yr, similar outcomes were observed: ß-cyclocitral or the mixture of the H. uvarum blend did not improve the attractiveness of the Fermentation lure or its selectivity. This study demonstrates that cues from different sources may interfere with each other and reduce D. suzukii attraction to otherwise attractive odor combinations.

Intraspecific Competition Affects the Pupation Behavior of Spotted-Wing Drosophila (Drosophila suzukii).

In Drosophila, intraspecific competition (IC) may cause stress, cannibalism, and affect survival and reproduction. By migrating to less crowded environments, individuals can escape IC. Larvae of spotted-wing drosophila (SWD, Drosophila suzukii) are often exposed to IC. They are known to pupate either attached to or detached from their hosts. Here, we hypothesized that SWD pupates detached from the larval host as a means to escape IC and increase their survival and fitness. Under laboratory conditions, IC resulted in increased pupation detached from the larval host in both cornmeal medium and blueberry fruit. Males were more prone to detached pupation than females. In blueberry, IC-exposed larvae pupated farther away from the fruit relative to singly-developed individuals. Detached pupation was associated to survival and fitness gains. For example, larvae that displayed detached pupation showed shorter egg-pupa development times, higher pupa-adult survival, and larger adult size relative to fruit-attached individuals. These findings demonstrate that SWD larvae select pupation sites based on IC, and that such a strategy is associated with improved survival and fitness. This information contributes to a better understanding of SWD basic biology and behavior, offering insights to the development of improved practices to manage this pest in the field.