Terrabacter carboxydivorans sp. nov., a carbon monoxide-oxidizing actinomycete.

A bacterial strain, PY2(T), capable of oxidizing carbon monoxide, was isolated from a soil sample collected from a roadside at Yonsei University, Seoul, Korea. On the basis of 16S rRNA gene sequence analysis, strain PY2(T) was shown to belong to the genus Terrabacter and was most closely related to Terrabacter lapilli LR-26(T) (99.1 % similarity). Strain PY2(T) was characterized chemotaxonomically as having iso-C(15 : 0) as the predominant fatty acid, MK-8(H(4)) as the major menaquinone, ll-diaminopimelic acid as the diagnostic diamino acid of the cell wall, as possessing a polar lipid profile that included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and unknown amino-containing phosphoglycolipids, and having a DNA G+C content of 75.6 mol%. DNA-DNA relatedness values between strain PY2(T) and the type strains of T. lapilli, Terrabacter tumescens, Terrabacter terrae and Terrabacter aerolatus were 20.0 %, 22.9 %, 35.9 % and 64.5 %, respectively. Based on the combined evidence from the phylogenetic analyses, chemotaxonomic data and DNA-DNA hybridization experiments, it is proposed that strain PY2(T) represents a novel species for which the name Terrabacter carboxydivorans sp. nov. is proposed. The type strain is PY2(T) (=KCCM 42922(T)=JCM 16259(T)).

Tsukamurella carboxydivorans sp. nov., a carbon monoxide-oxidizing actinomycete.

A Gram-positive, slightly acid-alcohol-fast, carbon monoxide-oxidizing bacterium, strain Y2(T), was isolated from a soil sample collected from a roadside in Seoul, Korea. On the basis of 16S rRNA gene sequence comparative analyses, strain Y2(T) was shown to belong to the genus Tsukamurella and was most closely related to Tsukamurella tyrosinosolvens DSM 44234(T) (GenBank accession no. AY238514; 99.8 %). The predominant fatty acids were C(18 : 1)omega9c and C(16 : 0). The cell-wall peptidoglycan of strain Y2(T) contained meso-diaminopimelic acid as the diagnostic diamino acid. Strain Y2(T) contained galactose and arabinose as the whole cell sugars. The DNA G+C content was 77 mol%. The DNA-DNA relatedness value between strain Y2(T) and T. tyrosinosolvens DSM 44234(T) was 62.7 %. Based on the combination of the carbon source utilization pattern, fatty acid profile, cell-wall chemotype, DNA G+C content and DNA-DNA hybridization experiments, it is proposed that strain Y2(T) (=KCCM 42885(T)=JCM 15482(T)) represents the type strain of a novel species, Tsukamurella carboxydivorans sp. nov.

Pseudonocardia carboxydivorans sp. nov., a carbon monoxide-oxidizing actinomycete, and an emended description of the genus Pseudonocardia.

A bacterial strain, Y8(T), capable of oxidizing carbon monoxide, was isolated from a soil sample collected from a roadside in Seoul, Korea. On the basis of 16S rRNA gene sequence similarity analyses, strain Y8(T) was shown to belong to the genus Pseudonocardia and was related most closely to the type strain of Pseudonocardia alni (99.6 % similarity). The cells were aerobic and stained Gram-positive, with white aerial mycelium and brown substrate mycelium. The predominant fatty acids were 16 : 0 iso and 16 : 1 iso. The cell-wall peptidoglycan of strain Y8(T) contained meso-diaminopimelic acid. The DNA G+C content was 77 mol%. Strain Y8(T) contained MK-9 as the major menaquinone, which is different from the major menaquinone reported previously in the genus Pseudonocardia, MK-8(H(4)). DNA-DNA relatedness between strain Y8(T) and the type strains of P. alni and Pseudonocardia antarctica was respectively 10 and 63 %. Based on phylogenetic, morphological and chemotaxonomic evidence, it is proposed that strain Y8(T) (=KCCM 42678(T) =JCM 14827(T)) be classified as the type strain of a novel species, Pseudonocardia carboxydivorans sp. nov. An emended description of the genus Pseudonocardia is also presented.