RESUMO
BACKGROUND: The clinical and pathological heterogeneity of progressive supranuclear palsy (PSP) is well established. Even with a well-defined clinical phenotype and a thorough laboratory workup, PSP can be misdiagnosed, especially in its early stages. CASE REPORT: A 52-year-old woman, who we initially diagnosed with a behavioral variant of frontotemporal dementia developed parkinsonian features, which then progressed to gait instability and gaze abnormality. CONCLUSIONS: We report herein a pathologically confirmed case of PSP presenting with behavioral changes including agitation and irritability, which eventually led to the cardinal symptoms of progressive supranuclear palsy.
RESUMO
Although there is no sequence information, activity-based screening methods can select positive clones from a metagenomic library. However, the low frequency of positive hits that is caused by improper expression of proteins in the cloning host Escherichia coli might be improved. In order to investigate whether the metagenome can be expressed in E. coli, the structural organization of URFs from metagenome was analyzed in terms of transcription and translation factors, and compared to those of 4300 ORFs of E. coli K12. Considerable differences in amino acid composition and codon usage occurred between the metagenome URFs and E. coli ORFs, reflecting a barrier for protein expression within the host E. coli. From the analyses of the promoter and RBS regions, sequences or patterns in the corresponding region of metagenome URFs were found to be dissimilar to E. coli consensus. These results suggested that these factors are considerable to screen the clones from metagenomic library with the activity-based approach.
