Non-contact oxygen saturation monitoring for wound healing process using dual-wavelength simultaneous acquisition imaging system.

Here we report the fabrication of a noncontact pulse oximeter system based on a dual-wavelength imaging system and its oxygen saturation monitoring performance during wound healing. The dual-wavelength imaging system consists of 660 nm and 940 nm light-emitting diodes and a multi-spectral camera that simultaneously accepts visible and near-infrared images. Using the proposed system, images were acquired at 30 fps at both wavelengths, and photoplethysmography signals were extracted by specifying a specific region in the images. We removed the signals caused by small movements and smoothed them using the discrete wavelet transform and moving average filter. To confirm the feasibility of the proposed noncontact oxygen saturation system, a wound model was created using a hairless mouse and oxygen saturation was measured during wound healing. The measured values were compared and analyzed using a reflective animal pulse oximeter. Through a comparative analysis of these two devices, the error of the proposed system was evaluated and the possibility of its clinical application and wound healing monitoring through oxygen saturation measurement confirmed.

Real-Time Monitoring of Colorectal Cancer Location and Lymph Node Metastasis and Photodynamic Therapy Using Fucoidan-Based Therapeutic Nanogel and Near-Infrared Fluorescence Diagnostic-Therapy System.

We report real-time monitoring of colorectal cancer, lymph node metastasis of colorectal cancer cells, and tumor growth inhibition through photodynamic therapy (PDT) using a near-infrared fluorescence diagnostic-therapy system with a light source for PDT and a fucoidan-based theranostic nanogel (CFN-gel) with good accumulation efficiency in cancer cells. To confirm the effect of the fabricated system and developed CFN-gel, in vitro and in vivo experiments were performed. Chlorin e6 (Ce6) and 5-aminolevulinic acid (5-ALA) were used for comparison. We confirmed that CFN-gel has a high accumulation efficiency in cancer cells and high fluorescence signals in near-infrared light for a long period, and only CFN-gel delayed the growth rate of cancer in terms of its size in PDT. In addition, using the near-infrared fluorescence diagnostic-therapy system and CFN-gel prepared for these experiments, the lymph node metastasis of cancer cells was imaged in real time, and the metastasis was confirmed through H&E staining. The possibility of image-guided surgery and identification of lymph node metastasis in colorectal cancer can be confirmed through CFN-gel and a near-infrared fluorescence diagnostic-therapy system that includes various light sources.