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A 71-year-old woman presented 2 weeks after vaccination with the first dose of Vaxzevria (AstraZeneca, Oxford) for COVID-19 with a left lower motor neuron facial nerve palsy, which progressed to bilateral involvement. This was accompanied by bilateral proximal leg weakness. She was diagnosed with the 'facial diplegia with paraesthesia' variant of Guillain-Barré syndrome. Seven weeks post vaccination she developed painless loss of vision in the right eye. The visual acuity in that eye was light perception only with a right relative afferent pupillary defect and right optic disc swelling. A diagnosis of optic neuritis was made and she received pulsed intravenous methylprednisolone for 3 days, followed by oral prednisolone. The optic neuritis recurred following initial cessation of steroids requiring an extended course of steroids. Despite this, she made a good visual recovery to 6/6 in the affected eye. We present this case and a review of the literature surrounding vaccination and the development of these conditions.
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Menu-based 'nudges' hold promise as effective ways to encourage a shift away from ruminant meat and towards more environmentally friendly plant-based food when dining out. One example of a menu-based nudge is including an inferior 'decoy' option to existing items on menus. Decoys have been shown to influence decision-making in other domains (e.g. Lichters, Bengart, Sarstedt, & Vogt, 2017), but have yet to be used to promote sustainable food choices. Two online randomized controlled trials tested whether the addition of higher priced 'decoy' vegetarian options on menus influenced the number of diners choosing a 'target' vegetarian option. Adjusted Generalized Estimating Equations on data from four menu conditions showed no main effect of the intervention in study 1 (decoy absent vs. decoy present; Odds Ratio (OR) 1.08 95% Confidence Interval (CI) 0.45 to 2.57). Replicating the trial in study 2 across seven menu conditions and testing a more expensive decoy also showed no main effect of the intervention decoy absent vs. decoy present; OR 0.68 (95% CI 0.41 to 1.12). Further analyses revealed that our price-based decoy strategy (a 30% price increase) did not significantly influence the number of people choosing the inferior decoy dish, possibly because dish choices were purely hypothetical. Further research is needed to clarify which attributes of a dish (e.g. taste, portion size, signature ingredients etc.) are optimal candidates for use as decoys and testing these in real world choice contexts.
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Comportamento do Consumidor/economia , Custos e Análise de Custo , Dieta Vegetariana/psicologia , Preferências Alimentares/psicologia , Planejamento de Cardápio/métodos , Adolescente , Adulto , Idoso , Comportamento de Escolha , Tomada de Decisões , Dieta Vegetariana/economia , Feminino , Rotulagem de Alimentos/economia , Rotulagem de Alimentos/métodos , Humanos , Masculino , Planejamento de Cardápio/economia , Pessoa de Meia-Idade , Razão de Chances , Restaurantes , Adulto JovemRESUMO
PurposeAnterior approach white line advancement presents a novel surgical option for correction of blepharoptosis. The technique draws on several advantages of other approaches. The aim of this study was to present outcomes using this technique at a minimum follow-up of 18 months.Patients and methodsParticipants having undergone anterior approach white line advancement ptosis correction at a single institution were retrospectively recruited at a minimum of 18 months' follow-up. A total of 18 independent eyelid measurements were recorded at final review. Outcomes included long-term rate of surgical success, upper eyelid margin-reflex distance (MRD1) at both early and late post-operative follow-up, inter-eyelid asymmetry, complications, re-operation rate, patient satisfaction, and quality-of-life improvement using the Glasgow Benefit Inventory (GBI). Pre- and post-operative MRD1, as well as inter-eyelid asymmetry, were compared using a two-tailed t-test.ResultsIn total, 82 eyelids of 47 participants were included with a mean follow-up of 2.3 years (range 1.5-3.7). Surgical success was achieved in 91.5%, with a final mean MRD1 of 3.5 mm (95% confidence 3.2-3.7). An increase of 2.4 mm (2.1-2.8) in eyelid height was observed between baseline and long-term follow-up (P<0.0001). No significant change was observed between early and late post-operative follow-up. Pre-operative asymmetry was reduced from 1.0 mm (0.7-1.3) to 0.4 mm (0.3-0.5; P<0.0001). Patient satisfaction was 95.7% with a mean GBI score of +21.8 (13.2-30.3).ConclusionsAnterior approach white line advancement presents an excellent option for patients undergoing ptosis correction with favourable long-term results. Comparisons are made with other techniques with respect to anatomical, functional, and surgical factors.
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Segmento Anterior do Olho/cirurgia , Blefaroplastia/métodos , Blefaroptose/cirurgia , Pálpebras/cirurgia , Músculos Oculomotores/cirurgia , Satisfação do Paciente , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Blefaroptose/fisiopatologia , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Músculos Oculomotores/fisiopatologia , Reoperação , Estudos Retrospectivos , Fatores de Tempo , Resultado do Tratamento , Adulto JovemRESUMO
Allogeneic hematopoietic cell transplantation (HCT) may produce long-term survival in AML after relapse or primary induction failure (PIF). However, outcomes of HCT performed for AML not in remission are historically poor given high relapse rates and transplant-related mortality. Preliminary studies suggest conditioning with clofarabine and myeloablative busulfan (CloBu4) may exert significant anti-leukemic effects without excessive toxicity in refractory hematologic malignancies. A prospective multicenter phase II trial was conducted to determine the efficacy of CloBu4 for patients proceeding directly to HCT with AML not in remission. Seventy-one patients (median age: 56 years) received CloBu4. At day 30 after HCT, 90% achieved morphologic remission. The incidence of non-relapse mortality and relapse at 2 years was 25% and 55%, respectively. The 2-year overall survival (OS) and event-free survival (EFS) were 26% and 20%, respectively. Patients entering HCT in PIF had significantly greater EFS than those in relapse (34% vs 8%; P<0.01). Multivariate analysis comparing CloBu4 with a contemporaneous cohort (Center for International Blood and Marrow Transplantation Research) of AML not in remission receiving other myeloablative conditioning (n=105) demonstrated similar OS (HR: 1.33, 95% confidence interval: 0.92-1.92; P=0.12). HCT with myeloablative CloBu4 is associated with high early response rates and may produce durable remissions in select patients with AML not in remission.
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Nucleotídeos de Adenina/administração & dosagem , Arabinonucleosídeos/administração & dosagem , Bussulfano/administração & dosagem , Transplante de Células-Tronco Hematopoéticas , Leucemia Mieloide Aguda/mortalidade , Leucemia Mieloide Aguda/terapia , Condicionamento Pré-Transplante , Adulto , Idoso , Aloenxertos , Clofarabina , Intervalo Livre de Doença , Feminino , Humanos , Quimioterapia de Indução , Masculino , Pessoa de Meia-Idade , Taxa de SobrevidaAssuntos
Transformação Celular Neoplásica/efeitos dos fármacos , Histona-Lisina N-Metiltransferase/antagonistas & inibidores , Leucemia Aguda Bifenotípica/tratamento farmacológico , Proteína de Leucina Linfoide-Mieloide/antagonistas & inibidores , Proteína de Leucina Linfoide-Mieloide/fisiologia , Proteínas de Fusão Oncogênica/fisiologia , Proteínas Proto-Oncogênicas/antagonistas & inibidores , Animais , Linhagem Celular Tumoral , Humanos , Leucemia Aguda Bifenotípica/patologia , CamundongosRESUMO
PURPOSE: To assess the long term impact of ptosis surgery on health-related quality-of-life using the Glasgow Benefit Inventory (GBI) patient-reported outcome measure. PATIENTS AND METHODS: A retrospective case note review was carried out on all patients who had undergone ptosis surgery (6 November 2008 to 5 December 2010) by one surgeon at Royal Bournemouth Hospital. Patient demographics, surgical technique, and complications were recorded. The GBI questionnaire data was obtained by telephone interview. RESULTS: A total of 62 ptosis operations (33 right, 29 left, of which 18 were bilateral) on 44 patients; 20 male, 24 female were included. Median age 77 years (range: 17-95 years). One wound dehiscence occurred, but no redo ptosis procedures were required. GBI data was obtained on 32 patients (45 ptosis procedures) at a mean follow-up period of 42.8 months postoperatively (range 31-67 months). The mean total GBI score for ptosis surgery was +21.36 (range 0-58.33; 95% confidence interval: 17.28-25.43, P<0.05). CONCLUSION: Patients derived a highly significant benefit to their health-related quality-of-life from ptosis surgery measured by the GBI that was maintained for up to 5 years.
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Blefaroplastia/psicologia , Blefaroptose/psicologia , Blefaroptose/cirurgia , Qualidade de Vida/psicologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Nível de Saúde , Humanos , Masculino , Pessoa de Meia-Idade , Satisfação do Paciente , Estudos Retrospectivos , Inquéritos e QuestionáriosRESUMO
A new trocar has been developed that increases the ease and accuracy of Lester Jones tube placement using the Cox system in conjunctivodacryocystorhinostomy (CDCR) surgery.
Assuntos
Túnica Conjuntiva/cirurgia , Dacriocistorinostomia/métodos , Intubação/instrumentação , Ducto Nasolacrimal/cirurgia , Desenho de Equipamento , HumanosRESUMO
PURPOSE: To assess the information included in optometrist referrals for cataract to the Hospital Eye Service with reference to the recommendations made by the Department of Health in "Action on Cataracts" and the resultant surgical listing rates. METHODS: We prospectively collected and analysed the information included in three different types of optometrist referrals for cataract over 8 weeks. Referrals containing "full information" as recommended in "Action on Cataracts" confirmed cataract as the cause of visual loss with a detrimental effect on the patient's lifestyle, and a willingness for surgery. The referral outcomes were assessed in terms of listing rate and the reasons for not listing, for each type of referral form. RESULTS: A total of 412 referrals were analysed. Of these, 50% (208) were "conventional" referrals via the general practitioner (GP) [39% (n = 162) using the General Ophthalmic Services (GOS) 18 referral form and 11% (n = 46) an optometrist's custom letter]; 35% (n = 143) were direct referrals and 15% (n = 61) were GP referrals with no optometrist information (these results were excluded from further analysis). The listing rates for optometric referrals were 78% (n = 272) overall, 83% (n = 119) for direct and 74% (n = 153) for conventional referrals. "Full information" was included in 17% (n = 8) of letter referrals and 10% (n = 16) of GOS 18 referrals. The listing rates were 83% for referrals with full information and 73% for referrals specifying only that cataracts were the cause of visual loss. The listing rates for referrals with partial information were 78% for "cataract" plus "effect on lifestyle" and 67% for "cataract" plus "willingness for surgery". The most common reason for not listing a patient for surgery was "no effect on lifestyle", accounting for 42% (n = 32). CONCLUSION: We recommend that all referrals for cataract should confirm a detrimental effect on lifestyle and the patient's willingness for surgery, in addition to confirming cataract as the main cause of visual loss.
Assuntos
Catarata/terapia , Optometria , Guias de Prática Clínica como Assunto , Encaminhamento e Consulta/normas , Extração de Catarata , Departamentos Hospitalares , Humanos , Oftalmologia , Estudos ProspectivosRESUMO
PURPOSE: Digital images are increasingly being used in ophthalmology. These may be viewed either on thin-film transistor (TFT) or on cathode-ray tube (CRT) displays. However, there is little data showing which is superior. In this study, we compared the performance of CRT and TFT displays for grading of both compressed and uncompressed images of diabetic retinopathy. METHODS: A total of 49 35 mm transparencies of diabetic retinopathy were scanned and compressed. The images comprised 17 with no retinopathy, eight with background, five with preproliferative, and 19 with proliferative retinopathy. Four levels of compression were used: 0, 70, 80, and 90%. A total of 196 randomised images were presented to two masked graders using both TFT and CRT displays under uniform lighting conditions, 2 months apart. The grade of retinopathy was assessed. Statistical analysis of grading accuracy was performed using receiver operator characteristic curves of sensitivity and specificity and the Stuart-Maxwell test for paired, nonparametric data. RESULTS: Both displays showed high sensitivity and specificity for the detection of any retinopathy. For the specific grade of retinopathy, the CRT performed slightly better with a sensitivity of 0.80 for uncompressed images, compared with 0.75 using the TFT. Compression reduced these sensitivities to 0.73 on the CRT and 0.63 on the TFT. Grading of uncompressed images magnified to four times their original size was more accurate on the TFT. CONCLUSIONS: Grading on both displays met sensitivity and specificity criteria proposed by Diabetes UK (formerly British Diabetic Association) for screening of diabetic retinopathy. The CRT generally performed slightly better than the TFT in relation to the detection of the specific grade of retinopathy.
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Periféricos de Computador , Retinopatia Diabética/diagnóstico , Compressão de Dados , Humanos , Processamento de Imagem Assistida por Computador , Programas de Rastreamento/instrumentação , Curva ROC , Sensibilidade e Especificidade , Índice de Gravidade de DoençaRESUMO
Two siblings with Ramon syndrome (MIM 266270) were found at follow-up to have anterior and posterior segment ocular abnormalities, including Axenfeld anomaly, retinopathy, and pale anomalous optic discs. These features were not described in the original report on this family. A recent report of another family with Ramon syndrome describes pigmentary retinopathy and optic disc pallor. It may be concluded that ocular abnormalities are another feature of this syndrome with anterior chamber abnormalities present at birth and retinopathy developing later.
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Anormalidades do Olho/diagnóstico , Anormalidades do Olho/genética , Adulto , Saúde da Família , Feminino , Seguimentos , Humanos , Masculino , Retina/anormalidades , SíndromeRESUMO
AIMS: To compare stereophotographic and monophotographic optic disc assessments made using a digital optic disc stereo camera. METHODS: Stereo digital optic disc photographs of 150 selected patients who had presented to a glaucoma clinic were assessed by two masked observers on separate occasions using (1) the stereophotographs and a stereoviewer, (2) a single image from the same stereopair. Results were analysed for both right and left eyes separately. 95% tolerance limits for change (TC) and intraclass correlation coefficients (ICC) were calculated and a multivariate analysis using a general linear model for repeated measures was performed. RESULTS: A total of 201 optic disc images of 150 patients (84 females, 108 left eyes) were analysed. Mean age of patients was 64 years. The results for right eyes are as follows (similar results were obtained for left eyes). Intraobserver (stereoscopic compared to monoscopic) measurements of: horizontal cup:disc ratios (CDR), ICC = 0.5995 and 0.7269, TC = 34% and 27%; vertical CDR, ICC = 0.8298 and 0.817, TC = 25% and 27%; area CDR, ICC = 0.7757 and 0.8259, TC = 28% and 25%; circumference CDR, ICC = 0.7618 and 0.8103, TC = 28% and 25%. Interobserver measurements of: horizontal CDR, ICC stereoscopic (SS) = 0.7287; monoscopic (MS) = 0.5030; TC SS = 30%; MS = 32%; vertical CDR, ICC SS = 0.8439; MS = 0.7106; TC SS = 25%; MS = 31%; area CDR, ICC SS = 0.8392; MS = 0.6276; TC SS = 26%; MS = 32%; circumference CDR, ICC SS = 0.8433; MS = 0.6438, TC SS = 26%; MS = 31%. Systematic bias between observers and between methods was within acceptable limits. CONCLUSIONS: This study using a digital stereo camera indicates that there may be little benefit of stereoscopic imaging over monoscopic imaging despite demonstrating small but inconsistent differences between both observers and methods.
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Glaucoma/patologia , Disco Óptico/patologia , Fotografação/métodos , Visão Binocular/fisiologia , Visão Monocular/fisiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Modelos Lineares , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Variações Dependentes do Observador , Reprodutibilidade dos TestesAssuntos
Catarata/etiologia , Lentes Intraoculares , Falha de Prótese , Idoso , Humanos , Masculino , Reoperação , Elastômeros de SiliconeRESUMO
BACKGROUND/AIMS: There are no nationally agreed guidelines on preoperative management of patients who are on aspirin or warfarin therapy. There is considerable evidence that complication rates in anticoagulated patients are low whereas there are higher rates of thromboembolic complications in those whose therapy is manipulated. This survey aimed to establish oculoplastic specialist and non-specialist ophthalmic surgeons' current management practice of patients before oculoplastic surgery who are taking aspirin or warfarin and to assess the rate of complications in these patients. METHOD: An anonymous postal questionnaire survey of all ophthalmic consultants and specialist registrars in the Wessex region along with oculoplastic specialists in the Southern region. RESULTS: The overall response rate was 92%. Preoperative management was influenced both by type of operation and by type of surgeon. A statistically significant higher proportion of surgeons would consider altering warfarin compared with aspirin treatment. For all procedures, non-specialists are unlikely to stop aspirin therapy, and are less likely to stop warfarin before all procedures apart from dacrocystorhinostomy. A significant proportion of surgeons (18%) would allow insufficient time for the coagulation status of the patient to change after altering treatment. A considerable proportion of surgeons (54%) reported that they had seen complications as a result either of stopping or continuing anticoagulation therapy. CONCLUSIONS: In this survey, at least half the surgeons questioned would consider stopping warfarin before oculoplastic procedures. Over half of all surgeons have seen complications related to aspirin or warfarin, some of which were serious. A suggested approach to minimising patient risk is given.
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Anticoagulantes , Aspirina , Procedimentos Cirúrgicos Oftalmológicos , Inibidores da Agregação Plaquetária , Varfarina , Anticoagulantes/efeitos adversos , Aspirina/efeitos adversos , Contraindicações , Humanos , Inibidores da Agregação Plaquetária/efeitos adversos , Cuidados Pré-Operatórios/métodos , Prática Profissional , Gestão de Riscos/métodos , Inquéritos e Questionários , Varfarina/efeitos adversosAssuntos
Infecção Hospitalar/transmissão , Infecções Oculares Bacterianas/transmissão , Unidades de Terapia Intensiva , Ceratite/microbiologia , Infecção Hospitalar/prevenção & controle , Infecções Oculares Bacterianas/prevenção & controle , Humanos , Ceratite/prevenção & controle , Fatores de RiscoRESUMO
PURPOSE: Early phase keratoconic corneas and their cultured keratocytes abnormally produce the Mr 62,000 form of the matrix metalloproteinase-2 (MMP-2). It is known that platelet derived growth factor (PDGF) and transforming growth factor-beta (TGF-beta) are involved in the regulation of MMP activity and tissue inhibitor of metalloproteinase (TIMP) production in non-ocular tissues. The purpose of this enquiry was to determine whether these growth factors also play a role in the activity and/or production of corneal MMP-2 and TIMP, and whether their activity could account for the existence of the Mr 62,000 form of MMP-2 in keratoconic corneas. METHODS: Confluent cultures of normal and early-phase keratoconic corneal keratocytes were established and incubated in serum-free media in the presence or absence of PDGF and TGF-beta. The proteins secreted by these cells over periods of 7 days were harvested for analysis. The total protein produced was determined spectrophotometrically. MMP-2 was visualised by SDS-gelatin polyacrylamide gel electrophoresis and assayed using radiolabelled type IV collagen as substrate. The enzyme inhibitors, TIMP-1 and TIMP-2, were quantified by dot blot immunoassay. RESULTS: The addition of PDGF or TGF-beta to the culture medium of keratoconic corneal keratocytes had no significant effect on overall protein production, MMP-2 activity or on the amounts of TIMP- 1 and TIMP-2 secreted. These observations also applied to normal corneal keratocytes, with the exception that PDGF induced expression of the Mr 62,000 species of MMP-2. CONCLUSIONS: PDGF may be involved in the production of the Mr 62,000 species of MMP-2 that is abnormally produced by early-phase keratoconic corneal keratocytes.
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Córnea/enzimologia , Fibroblastos/enzimologia , Ceratocone/enzimologia , Metaloproteinase 2 da Matriz/biossíntese , Células Cultivadas , Córnea/citologia , Meios de Cultura Livres de Soro , Eletroforese em Gel de Poliacrilamida , Fibroblastos/efeitos dos fármacos , Humanos , Ceratocone/patologia , Peso Molecular , Fator de Crescimento Derivado de Plaquetas/farmacologia , Inibidor Tecidual de Metaloproteinase-1/biossíntese , Inibidor Tecidual de Metaloproteinase-2/biossíntese , Fator de Crescimento Transformador beta/farmacologiaRESUMO
PURPOSE: This experiment reports the independence of first- and second-order processing mechanisms in form perception. METHODS: Symmetrical dot patterns were created using either luminance-increment dots (luminance above background), or texture-defined dots (average luminance equal to background). The proportion of luminance increment or texture dots defining each pattern was varied among fields of noise dots of the same type to determine symmetry detection thresholds. RESULTS: Differences in detection thresholds were found between luminance- and texture-defined patterns. Further, symmetry detection thresholds for luminance-increment dot patterns were resistant to noise defined by dots of opposite contrast polarity (luminance-decrement dots) or texture, while texture-defined patterns were resistant to neither texture nor luminance-decrement noise. CONCLUSIONS: These data suggest that symmetry perception, along with other types of form perception, use both first- and second-order processing mechanisms. The data are compatible with a second-order system that includes a negative half-wave rectifying non-linearity.
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Sensibilidades de Contraste/fisiologia , Percepção de Forma/fisiologia , Luz , Córtex Visual/fisiologia , Sinais (Psicologia) , Humanos , Limiar SensorialRESUMO
BACKGROUND: In the 4 year period (1988-91 there were nine cases of bacterial keratitis in five critically ill patients on an intensive care unit ('unit A'), all except one due to Pseudomonas aeruginosa. Many of these patients had serious ocular complications requiring surgery and all surviving patients were left with significant visual deficits. One further case of keratitis due to P aeruginosa occurred on unit A in April 1993. The problem of keratitis in ventilated patients is not unique to this unit as a further four cases in three patients from additional units in this area have been treated. METHODS: Predisposing factors in unit A were established through subsequent investigations. It was found, in particular, that all the ocular infections were preceded by colonisation of the respiratory tract with the pathogenic organism. Recommendations concerning eye care and tracheal suctioning were adopted by unit A in 1991. RESULTS: In the subsequent 4 years (1991-5), the frequency of isolation of pseudomonas from the respiratory tract per patient treated in unit A remained relatively high at 3.8% (153/4032). However, the conjunctival pseudomonas isolation rate has decreased significantly (p < 0.001) from 0.8% (19/2430) to 0.05% (2/4032). CONCLUSIONS: Ventilated patients may be at risk from inoculation of pathogens into the eyes. The principal risk factor for bacterial keratitis in this series was corneal exposure secondary to conjunctival chemosis or lid damage. The adoption of simple preventative measures on unit A had a significant impact on the incidence of eye infections due to pseudomonas, despite the high proportion of patients whose respiratory tracts were colonised with the same organism. There is a need for additional research into the most effective method of eye care for ventilated patients in order to reduce the frequency of this avoidable condition.
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Estado Terminal/terapia , Infecção Hospitalar/transmissão , Infecções Oculares Bacterianas/transmissão , Ceratite/microbiologia , Infecções por Pseudomonas/transmissão , Cuidados Críticos/métodos , Infecção Hospitalar/prevenção & controle , Infecções Oculares Bacterianas/prevenção & controle , Humanos , Ceratite/prevenção & controle , Guias de Prática Clínica como Assunto , Infecções por Pseudomonas/prevenção & controle , Respiração Artificial/efeitos adversos , Estudos Retrospectivos , Fatores de RiscoRESUMO
The percutaneous temporal bone pedestal has shown significant utility for the attachment of bone-anchored hearing aids, attachment of cosmetic auricular prostheses, and as connector between external sound processors and implanted cochlear implants. The biological acceptance of these implants by temporal bone hosts is affected by many factors including the maturity of the bone, the design of the pedestal-fixation system, and the pedestal-construction material. The first phase of this study evaluated the effect of the pyrolized graphite pedestal fixation on maturing temporal bones. Pedestals were implanted in young swine temporal bones using single screw and multiple screw fixation systems. The effect on temporal bone growth is demonstrated with photomicrographs and gross photography showing acceptable host-tissue response to the presence of multiple and single screw attachment techniques. Osseointegration of the attaching screws occurred. The second phase of the study evaluated osseointegration of smooth, beaded, and textured titanium pedestals in feline temporal bones. High-resolution temporal bone/pedestal sectioning has been accomplished with high-performance microtomes, showing the osseointegration of the pedestal by the temporal bone. This is demonstrated with tetracycline labeling and histologic assessment. Percutaneous pedestals are of increasing importance in otologic practice. This study assists in the understanding of biologic acceptance of pedestals as influenced by the pedestal composition and fixation design. This basic understanding is essential for design improvements in percutaneous temporal bone pedestals.
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Osseointegração , Osso Temporal/cirurgia , Animais , Próteses e Implantes , SuínosRESUMO
A polymerase chain reaction- (PCR) based short tandem repeat (STR) system has recently been developed for use in routine forensic identity testing. The methodology involves the simultaneous amplification of alleles at four loci on different chromosomes, followed by the fluorescent detection of products using an automated DNA sequencer. The adoption of this technology into operational casework offers several advantages over systems currently in use, particularly the ability to obtain results from very old or small samples, reduced operator time when compared with conventional DNA (single locus probe) analysis and the potential for automation. Validation studies were incorporated into the development work on this system. The scope of these studies has been extended by further investigation carried out in this laboratory to test the reliability of the system under normal operational procedures. It was demonstrated that the precision of size determination was sufficient for the discrimination of alleles and size windows for allelic designation were established. A collaborative exercise carried out in conjunction with two independent laboratories demonstrated the robustness of allelic designation. Having tested both the DNA quantification and amplification techniques against DNA samples from a wide range of animal and microbial species, it was confirmed that results are only obtained from higher primate DNA. The PCR methodology was tested with both simulated and real casework samples (over 250 in total). Reportable results were obtained from most items yielding extracted DNA. Approximately 20% of the casework items from which no grouping (ABO, PGM) nor SLP results were obtained, gave reportable STR results. A method for the routine purification of DNA extracts which failed to amplify was established and validated for use in forensic casework. The STR multiplex system developed by Kimpton et al. proved robust and reliable when tested under the operational procedures in place in this laboratory.