New polymerized complexes of amino-triazole with transition metals as potential fungicidal drugs.

New synthesized polymerized complexes of 3-amino-1,2,4-triazole with transition metals such as Cu+2, Ni+2, Co+2 and Zn+2 were investigated for fungicidal activity. Most of the tested complexes showed fungicidal activity which is not connected with activity of copper fungicides.

Susceptibility of gram-positive bacteria isolated from a paediatric hospital environment to antibiotics.

Susceptibility of Gram-positive bacteria isolated from the hospital environment to antibiotics was determined. About 19% strains were resistant to most tested antibiotics. Among 30 coagulase-negative staphylococci, 8 of them have been found to be resistant to 6-13 antibiotics, whereas among 15 strains of S. aureus only one was resistant to 6 antibiotics. Only gentamicin was effective to all tested Gram-positive strains, whereas ceftazidim, tetracycline and doxycycline were ineffective to the most tested bacteria.

Susceptibility of gram-negative bacteria isolated from a paediatric hospital environment to antibiotics.

The majority of tested Gram-negative strains isolated from the hospital environment were resistant to 6-14 used antibiotics. The greatest resistance was shown by three strains: Enterobacter cloacae, Proteus mirabilis and Ps. maltophilia, which were resistant to all tested drugs. The most effective antibiotic was gentamicin, at least against half of bacteria belonging to Enterobacteriaceae family, to 90% of strains from Pseudomonas genus and to all other Gram-negative rods. Cephtazidim, cephotaxim, colistin and carbenicillin were effective only to 60-70% of Enterobacteriaceae family strains, whereas ampicillin and tetracycline to 70% of Pseudomonas genus. Other Gram-negative bacilli were more susceptible to antibiotics. Cephalothin was ineffective to all tested strains.

Susceptibility of bacteria isolated from non-parenteral preparations applied in paediatric hospitals to antibiotics.

No strains resistant to all tested antibiotics were found among the 33 Gram-positive and 17 Gram-negative bacteria isolated from non-parenteral drugs and purified water. Three strains: S. aureus, coagulase-negative staphylococcus and B. cereus have been found to be resistant to 6-8 antibiotics, whereas six Gram-negative strains belonging to Enterobacter agglomerans and Ps. aeruginsosa were resistant to 6-12 antibiotics. The most effective agent against all tested Gram-positive bacteria was cephaloridine, and erythromycin against the staphylococci. Tetracycline and ceftazidim were the least effective. Gentamycin was the only antibiotic effective to all Gram-negative strains, and ceftazidim to most of them. Cefsulodin was ineffective to Ps. aeruginosa.

Some structure-activity relationships of iodophorous iodine complex compounds. Part II. Biological.

The iodophors described in the part I [5] were subjected to studies in order to determine their antibacterial activity against Staphylococcus aureus 209P, Pseudomonas aeruginosa and Escherichia coli. General effectiveness was evaluated and useful concentration was determined using the FDA (Food and Drug Administration) method and a test with mechanical carriers. The obtained results were compared with respective values for a commercial product--Iosan (Ciba--Geigy); we found that properties of the products were comparable. The possibility of removal of iodophors, labelled with 131I, from glass, rubber and polystyrene surfaces was also examined. We found that the iodophors are suitable for disinfection of glass and drug packages.

Studies on the sterilization of pharmaceutical base materials with ionizing radiation and ethylene oxide.

The use of ionizing radiation and ethylene oxide for the sterilization of pharmaceutical base materials of animal origin, used to produce organopreparations, was studied. The materials included liver extract, pancreas extract, dried thyroid and intrinsic factor. The effective sterilizing doses for the examined materials and dependence between effective ionizing radiation dose and primary contamination were determined.