Effects of a Multi-Suckling System Combined With Enriched Housing Post-Weaning on Response and Cognitive Resilience to Isolation.

Improving welfare is still a critical issue in pig husbandry. Upgrades of the housing environment seem to be a promising solution to optimise resilience as a whole, and therefore improve animal welfare. The objective of this study was to evaluate the effect of an alternative housing system to enhance cognitive resilience and also to promote the pigs' welfare. A total of 96 piglets from two contrasted housing systems [alternative housing system (AHS) vs. conventional system (CONV)] was used. The major upgrades of the alternative system were multi-litter housing during lactation, delayed weaning, extra space allowance, and environmental enrichment from birth onwards. To estimate welfare, weight, and feed intake (as a general indicator of performances), the tear staining area (as a chronic stress indicator), behavioural postures, heart rate traits, and saliva cortisol concentration were measured over a 21 h-isolation. To assess cognitive resilience, the pigs were subjected to a maze with a social reward both before and after the isolation challenge and indicators of cognitive abilities were followed. The AHS pigs showed lower cortisol levels and tear staining area before the challenge, demonstrating overall better welfare due to the alternative housing conditions. During the challenge, AHS pigs had a lower heart rate, higher heart rate variability, and higher vagal activity than the CONV pigs, which might indicate a reduced sensitivity to the stressor. AHS pigs appeared to have a better long-term memory tested in a maze. Providing social and environmental enrichments, that fit the satisfaction of the essential needs of the pigs better, appears to be beneficial for pig welfare as a whole. Its effects on cognitive resilience still need to be proven.

Impact of Enrichment and Repeated Mixing on Resilience in Pigs.

Resilience, the capacity of animals to be minimally affected by a disturbance or to rapidly bounce back to the state before the challenge, may be improved by enrichment, but negatively impacted by a high allostatic load from stressful management procedures in pigs. We investigated the combined effects of diverging environmental conditions from weaning and repeated mixing to create high allostatic load on resilience of pigs. Pigs were either exposed to barren housing conditions (B) from weaning onwards or provided with sawdust, extra toys, regular access to a "play arena" and daily positive human contact (E). Half of the pigs were exposed to repeated mixing (RM) and the other half to one mixing only at weaning (minimal mixing, MM). To assess their resilience, the response to and recovery from a lipopolysaccharide (LPS) sickness challenge and a Frustration challenge were studied. In addition, potential long-term resilience indicators, i.e. natural antibodies, hair cortisol and growth were measured. Some indications of more favorable responses to the challenges in E pigs were found, such as lower serum reactive oxygen metabolite (dROM) concentrations and a smaller area under the curve of dROM after LPS injection. In the Frustration challenge, E pigs showed less standing alert, escape behaviors and other negative behaviors, a tendency for a smaller area under the curve of salivary cortisol and a lower plasma cortisol level at 1 h after the challenge. Aggression did not decrease over mixings in RM pigs and was higher in B pigs than in E pigs. Repeated mixing did not seem to reduce resilience. Contrary to expectations, RM pigs showed a higher relative growth than MM pigs during the experiment, especially in the week of the challenges. Barren RM pigs showed a lower plasma cortisol concentration than barren MM pigs after the LPS challenge, which may suggest that those RM pigs responded less detrimentally than MM pigs. Enriched RM pigs showed a higher level of IgM antibodies binding keyhole limpet hemocyanin (KLH) than enriched MM and barren RM pigs, and RM pigs showed a sharper decline in IgG antibodies binding Bovine Serum Albumin (PC-BSA) over time than MM pigs. Hair cortisol concentrations were not affected by enrichment or mixing. To conclude, enrichment did not enhance the speed of recovery from challenges in pigs, although there were indications of reduced stress. Repeated as opposed to single mixing did not seem to aggravate the negative effects of barren housing on resilience and for some parameters even seemed to reduce the negative effects of barren housing.

Potential improvements of the cognition of piglets through a synbiotic supplementation from 1 to 28 days via the gut microbiota.

The influence of feed supplements on behavior and memory has been recently studied in livestock. The objectives of the study were to evaluate the effects of a synbiotic on: an episodic-like (SOR: Spontaneous Object Recognition), a working (BARR: Fence barrier task), a long-term (TMAZE: Spatial T-maze task) memory test and on gut microbiota composition. Eighteen female piglets were supplemented from 1 to 28 days of age with a synbiotic (SYN), while 17 served as control (CTL). Feces were collected on days 16, 33 and 41 for 16S rRNA gene composition analyses. In the SOR, SYN piglets interacted more quickly with the novel object than CTL piglets. In the BARR, SYN piglets had shorter distances to finish the test in trial 3. In the TMAZE, SYN piglets were quicker to succeed on specific days and tended to try the new rewarded arm earlier during the reversal stage. Difference of microbiota composition between treatments was nonexistent on D16, a tendency on D33 and significant on D41. The synbiotic supplement may confer memory advantages in different cognitive tasks, regardless of the nature of the reward and the memory request. Difference in memory abilities can potentially be explained by differences in microbiota composition.

Effects of Three Distinct 2-Week Long Diet Strategies After Transport on Weaned Pigs' Short and Long-Term Welfare Markers, Behaviors, and Microbiota.

Alternative feed supplements have shown promising effects in terms of performance, but their effects on welfare have had little evaluation. In the present study, we aimed at evaluating the effect of diet supplementation on welfare indicators. A total of 246 piglets were weaned and transported for 12 h. After transport, they were assigned to one of 3 diets for a 14-day period: A-an antibiotic diet including chlortetracycline and tiamulin, NA-a control diet without any antibiotic or feed supplement, GLN-a diet including 0.20% L-glutamine. After the 14-day period, all piglets were fed the same diet. Tear staining was measured 11 times post-weaning (from d0 to 147). Skin lesions were counted before and after weaning (d-2, 2, and 36). Novel object tests (NOT) were done in groups 4 times post-weaning (d17, 47, 85, 111). Samples for 16S rRNA gene composition were collected prior to transport (d0), following the 14-day period (d14) and at the conclusion of the nursery phase (d34). The NA pigs appeared less interested in novel objects. On d17, they avoided the object less than A pigs (P < 0.05). They spent less time exploring the object on d85 and took longer to interact with the object on d111 than A and GLN pigs (P < 0.05). NA pigs also appeared more sensitive to environment and management. They had larger tear stains than GLN pigs on d84 and 110 (P < 0.05). On d2, NA pigs had more lesions than A and GLN (P < 0.01). In terms of microbiota composition, GLN had higher α-diversity than A and NA (P < 0.001). Differences between dietary treatments were absent at d0, were demonstrated at d14 and disappeared at d34. Pearson correlations between aggression, stress and anxiety indicators and bacterial populations were medium to high from 0.31 to 0.69. The results demonstrate that short-term feeding strategy can have both short- and long-term effects on behavior and welfare, that may partly be explained by changes in gut microbiota composition. Supplementation with GLN appears to confer similar benefits to dietary antibiotics and thus could be a viable alternative.

Effect of Floor Cooling on Behavior and Heart Rate of Late Lactation Sows Under Acute Heat Stress.

Much U.S. swine production is in Köppen climate types classified as "hot-summer humid continental" and "humid subtropical." As a result, farrowing sows are often exposed to temperatures above their upper critical temperature. This heat stress (HS) can affect sow welfare and productivity and have a negative economic impact. The study objective was to evaluate the impact of a cooling pad on sows' behavioral and heart rate responses to acute HS. Treatments were randomly allotted to ten multiparous sows to receive a constant cool water flow of 0.00 (CONTROL, n = 4), 0.25 (LOW, n = 2), 0.55 (MEDIUM, n = 2), or 0.85 (HIGH, n = 2) L/min for 100 min and replicated eight times, switching treatments so that each sow was exposed to each treatment. The cooling was initiated 1 h after the room reached 35°C for 100 min. Eating, drinking and nursing behaviors, postures, and heart rate were recorded before heating (Period 1), prior to cooling (Period 2), and during cooling (Period 3). There were no differences between LOW, MEDIUM, and HIGH flow rates for any periods on all behavioral and heart rate traits, so data were pooled (COOLED). There were no differences in any of the measures during Periods 1 and 2, except for the ratio of short term to long term heart rate variability (SD1:SD2) with higher values for CONTROL than COOLED sows in Period 2. During Period 3, CONTROL sows changed postures more frequently (11.5 ±1.6 vs. 5.1 ±1.6 changes per hour), spent more time drinker-pressing/drinking (4.4 ± 0.5 vs. 1.4 ± 0.4% of time), standing (6.6 ± 1.7 vs. 3.8 ± 1.6% of time), sitting (10.0 ± 1.2 vs. 4.0 ± 1.1), less time lying (83.0 ±1.8 vs. 92.0 ±1.7% of time), especially lying laterally (62.0 ± 5.6 vs. 75.0 ± 5.3% of time), than sows in all three cooling treatments (all P < 0.001). Heart rate during Period 3 was lower for COOLED sows compared to the CONTROL sows (100.2 ± 3.4 vs. 119.0 ± 4.0 beat per min, P < 0.001). Sows response to increased thermal load can be effectively reduced using water-cooled cooling pads, thereby improving sow comfort and welfare. The beneficial effects on behavior are noticeable from the lowest flow rate.