RESUMO
BACKGROUND/AIM: Glioblastoma multiforme is an aggressive primary tumor that arises in the glial cells of the brain. Standardized first-line treatment has considerable morbidity and less than one-year median survival after intervention. Ultra-low intensity electromagnetic fields have been shown to interact with biological organisms without anticipated deleterious side-effects. The aim of the study was to determine if a novel, non-invasive application of non-ionizing radiation has an inhibitory effect on proliferation of glioblastoma multiforme cells. MATERIALS AND METHODS: U-87 MG cells were continuously exposed for 54 h to an electromagnetic field tuned to simultaneously interact with DNA/RNA oligonucleotides (mutated alpha-kinase 2 gene/Hsa-miR-381-5p respectively) and proteins (HSP70/CHI3L1). RESULTS: Exposed cells demonstrated a significant inhibition of cell growth and concurrent increase in cell death. CONCLUSION: This technology induces cell death by novel non-cytotoxic mechanisms unlikely to induce side-effects in patients; can be customized for individual tumors and may contribute to the emerging strategy of personalized medicine.
Assuntos
Pontos de Checagem do Ciclo Celular/efeitos da radiação , Proliferação de Células/efeitos da radiação , Campos Eletromagnéticos , Redes Reguladoras de Genes/efeitos da radiação , Neoplasias Encefálicas/genética , Neoplasias Encefálicas/metabolismo , Neoplasias Encefálicas/patologia , Pontos de Checagem do Ciclo Celular/genética , Linhagem Celular Tumoral , Proliferação de Células/genética , Sobrevivência Celular/genética , Sobrevivência Celular/efeitos da radiação , Perfilação da Expressão Gênica/métodos , Regulação Neoplásica da Expressão Gênica/efeitos da radiação , Redes Reguladoras de Genes/genética , Glioblastoma/genética , Glioblastoma/metabolismo , Glioblastoma/patologia , Humanos , MicroRNAs/genéticaRESUMO
The regulation of vertebrate eye development requires the activity of many transcription factors. In this report, we demonstrate that the T-box factor Tbx12 is necessary for normal development of the retina. Tbx12 is expressed during early stages of retinal development in multiple species of vertebrate embryos. We injected mRNAs encoding wild type and mutant forms of Tbx12 into Xenopus embryos. The Tbx12 injected embryos exhibit multiple defects in eye development including reduced eye size and disruption of normal retinal laminar organization. Tbx12 appears to function as a repressor of transcription during eye development. Our results indicate that Tbx12 activity is required for the proper generation and organization of retinal cells in the vertebrate eye.
Assuntos
Olho/embriologia , Proteínas com Domínio T/farmacologia , Proteínas com Domínio T/fisiologia , Xenopus laevis/embriologia , Animais , Olho/metabolismo , Expressão Gênica , Hibridização In Situ , Injeções , Camundongos , Mutação , Fenótipo , Epitélio Pigmentado Ocular/embriologia , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , RNA Mensageiro/farmacologia , Retina/citologia , Retina/embriologia , Retina/metabolismo , Proteínas com Domínio T/genética , Xenopus laevis/metabolismoRESUMO
Wnt signaling molecules regulate the development of multiple organs in vertebrate embryos. We have isolated cDNA clones for frizzled10 (Fz10), which encodes a putative Wnt receptor, to further characterize the mechanisms of Wnt signaling in mouse embryos. Interestingly, Fz10 is expressed in the same regions as Wnt7a in the neural tube, limb buds, and Müllerian duct.
Assuntos
Embrião de Mamíferos/metabolismo , Receptores de Superfície Celular/genética , Transdução de Sinais/fisiologia , Sequência de Aminoácidos , Animais , Extremidades/embriologia , Extremidades/crescimento & desenvolvimento , Receptores Frizzled , Regulação da Expressão Gênica no Desenvolvimento , Camundongos , Dados de Sequência Molecular , Receptores de Superfície Celular/metabolismo , Receptores Acoplados a Proteínas G , Homologia de Sequência de AminoácidosRESUMO
We have isolated the Xenopus orthologue of the T-box gene, Tbx20, and characterized its developmental expression profile. We show that Tbx20 is one of the earliest markers of heart tissue in Xenopus, and is expressed throughout all cardiac tissue during later stages of development. In addition, we also observe expression in the cement gland, the jugular vein, the lung bud, the cloacal aperture, rhombomeres 2, 4, 6 and 8, and in a subset of motor neurons.
