RESUMO
A procedure has been developed for simultaneous determination of the activities of alpha-D- and beta-D-mannosidase in the biological fluids from the quantity of free 4-nitrophenol. The latter is released via enzymatic degradation of substrates of 4-nitrophenyl-alpha-D-mannose and 4-nitrophenyl-beta-D-mannose in individual incubation tests.
Assuntos
Astenozoospermia/enzimologia , Duodenite/enzimologia , Gastrite/enzimologia , Espermatozoides/enzimologia , alfa-Manosidase/análise , beta-Manosidase/análise , Adolescente , Adulto , Animais , Astenozoospermia/diagnóstico , Criança , Duodenite/diagnóstico , Gastrite/diagnóstico , Humanos , Masculino , Nitrofenóis/metabolismo , Ratos , Adulto Jovem , alfa-Manosidase/sangue , alfa-Manosidase/metabolismo , beta-Manosidase/sangue , beta-Manosidase/metabolismoRESUMO
A procedure has been developed for simultaneous determination of the activity of a-L-fucosidase (EC 3.2.1.5 1) and the beta-D-fucosidase activity caused by beta-D-galactosidase (EC 3.2.1.23, Form IV) in serum. The paper presents data on changes in the above serum enzymatic activities in an experiment, in children with recurrent laryngotracheitis and in pregnant females with gestosis and placental insufficiency.
