[Pancreatic islets isolation from the brain death donors]. / Izolarea de insule pancreatice de la donator în moarte cerebrala.

The paper analyzes a number of 5 cases of pancreatic islet cells isolation, purification and culture extracted from the pancreas of brain-dead donors. For the isolation stage the authors used the "two-step" digestion method. The isolated pancreatic islet cells were morphologically and functionally analyzed by assessing their viability and purity. For the in-vivo experiments the authors used animals with Streptozotocin induced diabetes. The data clearly indicated a high efficiency of collection providing an optimal number of transplantable pancreatic islet cells.