RESUMO
Cell lines established from the biopsy specimen of a patient with a spindle B choroidal melanoma were transplanted into the posterior choroid of 30 immunosuppressed, pigmented New Zealand rabbits. Growth of the tumor xenografts could be seen 7 to 10 days after transplantation. Tumor xenografts were reproducible and reached an average size of 5 mm in height and 8 mm in their basal dimension by 4 weeks. Histopathology of the original tumor revealed primarily spindle B cells, while the tumor xenografts contained epithelioid, spindle B, and clear cell melanoma cells. Of particular interest was the presence of an extensive and intact vasculature and the absence of a capsule surrounding the lesions in situ. The use of human uveal melanoma cells, the ease of transplantation, and the posterior location of the tumor may make this animal model of use in studies on new diagnostic and therapeutic modalities.
Assuntos
Neoplasias da Coroide/patologia , Melanoma/patologia , Idoso , Idoso de 80 Anos ou mais , Animais , Neoplasias da Coroide/diagnóstico por imagem , Ciclosporina/administração & dosagem , Modelos Animais de Doenças , Enucleação Ocular , Feminino , Fundo de Olho , Rejeição de Enxerto/prevenção & controle , Humanos , Terapia de Imunossupressão , Melanoma/diagnóstico por imagem , Transplante de Neoplasias , Coelhos , Transplante Heterólogo , Células Tumorais Cultivadas , UltrassonografiaRESUMO
We examined the effect of liver damage induced by carbon tetrachloride on the incidence of hepatic metastasis in the Greene hamster melanoma. Nine of 17 animals with liver damage had melanoma growth in the liver after the intraportal vein injection of tumor cells, whereas tumor growth occurred in four of 27 control animals (P less than .01). These results suggest that, in this model, liver damage predisposes to metastatic involvement by melanoma.
