[Water loading diuresis test in albino rats. Critical study of approximately 12,000 tests. 3. Loading tests using several mineral waters bottled under various experimental conditions]. / Prove di diuresi da carico idrico nel ratto albino. Esame critico di circa 12,000 prove. Nota III: Prove da carico con alcune acque mineralizzate confezionate in bottiglia in varie condizioni sperimentali.

The authors studied a series of more than 12,000 complex diuresis exams after water loading, in the albino rat, which permitted them to draw the following conclusions: 1). The temperature of water definitively and, probably the environmental temperature, influence the diuretic response. 2). The most favourable water loading preparations are not fasting and solid fasting during the 24 hrs. prior to loading. 3). Pretreatment with diuretic water 15 days prior to loading, favours the diuretic response to the same or the any other water: activated kidney. 4). The addition of CO2 to water, by itself, does not seem to favour diuresis. 5). Aging or conservation in bottles, always reduces, more or less sensitively, the diuretic properties of the water itself, especially during the first 2-3 months. This confirms that the diuretic properties do not only and exclusively depend on the chemical or physical composition or on the structure, but on everything together, that is modified after the moment of it's natural emergence. This loss of activity, in any case, is favourable to the indiscriminate use of these waters as both dietetic and drinking water. 6). It is probable that the different pH's of water influence it's diuretic activity. 7). Even if the more active waters, in this study, seem to be bicarbonate, sulfate bicarbonate, or bicarbonate-sulfate-alkaline-terrose, this can be attributed to the prevalence of these classes of water in this study. Instead, what emerges with certainty, from the point of view of the molar concentration, is that while there are which are minimally mineralized and oligominerals with scarce or no diuretic activity, there are mediomineral and hypotonic mineral waters with up to 100 mmol/liter, and with conspicuous diuretic activity, even after more or less long periods of conservation in bottles. 8). For all of the above-mentioned reasons, we feel that a useful orientation can also be gained for the use of the discussed water for human needs.

[Weight increase and chronic experimental poisoning by tobacco smoke in the albino rat. Effect of protection with mineral waters]. / Incremento ponderale e intossicazione cronica sperimentale da fumo di tabacco nel ratto albino. Effetti della protezione con acque minerali.

The Authors refer to the results of an experiment conducted on albino rats chronically intoxicated with tobacco smoke. Several groups of animals were contemporaneously protected, by drinking or by inhaling or by both methods, by a beneficial sulphureous water with a varying sulphydrometric degree, and in one experiment, by inhaling a hypertonic salso-bromo-iodic water. In all cases, it was possible to observe that in the adopted experimental conditions, the chronic intoxication from tobacco smoke slowed the weight increase of albino rats subjected to intoxication and not protected, both as regards non-intoxicated animals and as regards intoxicated animals protected by one of the studied waters. There were no statistically significant differences between the weight increase of the animals protected by the drinking or inhaling methods while protection via both methods resulted statistically more efficacious than protection by one single method.

[First pharmacological studies of the properties of bottled Tesorino water]. / Prime ricerche farmacologiche sulle proprietà dell'acqua Tesorino conservata in bottiglia.

The Authors carried out a series of researches aiming to control, over time, the state of preservation and the biological properties of a magnesic-calcic-sulfate-bicarbonate medium-mineral water, also available on the market for dietetic purposes. The investigations allowed to demonstrate that when the bottles are opened the pH is stable on the starting values even for bottles opened after more than 400 days from bottling them. It was observed, however, that with bottles which had been left opened for many days, the pH of the older bottles was more stable, while the one of bottles only 50 days old tended to move towards alkaline values and to remain unchanged during the following days. The control of the zymosthenic properties in vitro, with amylase and trypsin on the relative substrates, made possible to demonstrate that the presence of the water under study considerably favours these enzymic processes, which need considerably smaller amounts of enzyme, in comparison with the tests performed on control solutions. Such effect is constantly present even after the water has been bottled for a long time. Tests on loading diuresis on albinic rats showed that the water under study favours a lot the kidney emuntory activity also in not previously treated animals. Animals previously watered with the medium-mineral water, however, in the loading tests with the various waters display a greater functional activity from the kidney "trained kidney". Moreover, the Authors point out that, with such water, the diuretic response seems to be improved of water is drunk after eating.