1.
Mutat Res
; 304(2): 181-5, 1994 Jan 16.
Artigo
em Inglês
| MEDLINE
| ID: mdl-7506360
RESUMO
We have developed and tested a simple phenotypic assay which monitors C to T transition mutations at the second C of a CCAGG sequence in the lacZ gene of Escherichia coli. The assay is based on new data concerning amino acid requirements on either side of a crucial active site residue in beta-galactosidase, glutamic acid 461. We show that the frequency of occurrence of the mutation is influenced by two genes: dcm, the cytosine methylase gene, and vsr, one of the genes involved in very short patch repair. The assay has been used to evaluate the function of vsr cloned from a potential very short patch repair mutant.