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Objective To investigate the correlation between the incidence of type 2 diabetes mellitus (T2DM) with hypertension and the levels of some elements in serum, and to provide a more scientific and reasonable basis for the control of T2DM. Methods A total of 1 024 T2DM patients admitted to a top three hospital in Urumqi from March 2015 to May 2016 were selected. Patients with T2DM were divided into T2DM with hypertension group and T2DM without hypertension group according to the presence or absence of hypertension. Various elements levels in the serum were analyzed. The correlation between serum levels and the incidence of hypertension with T2DM was analyzed, and the related risk factors were explored by using logistic regression. Results Among 1 024 T2DM patients, 627 patients had hypertension, accounting for 61.20%, while 397 patients had no hypertension, accounting for 38.80%. There was a positive correlation between serum sodium and magnesium levels with T2DM in hypertension (P<0.05). The increase of serum sodium level and the decrease of magnesium level were the risk factors of T2DM with hypertension. This result suggested that the difference of serum levels of various elements may play a role in the occurrence and the development of T2DM. Conclusions Serum sodium and magnesium levels were positively correlated with the incidence of T2DM with hypertension. The increase of serum sodium level and the decrease of magnesium level were influencing factors of the incidence of T2DM with hypertension. This study suggests that the serum levels of sodium and magnesium play a role in the occurrence and development of T2DM.
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The development of new generation sequencing technologies has brought new opportunities for the study of diseases. Exome sequencing has shown to be an effective, rapid, high performance technique that has already been used in research of inherited diseases such as monogenic disorders. It has already been approved by scientists in the field of monogenic disorder study, and will become widely used. This approach will accelerate discovery of the causative genes of Mendelian disorders. This article reviews some recent applications of exome sequencing in the study of gene-related diseases.
Assuntos
Exoma/genética , Estudos de Associação Genética , Doenças Genéticas Inatas/genética , Análise de Sequência de DNA , HumanosRESUMO
Objective To study the effects of nicotinamide on the human skin melanocytes and try to explore the potential mechanism of nicotinamide on the calcium signal transduction,cytoskeleton.Methods The primary cultured human skin melanocytes from foreskin were selected as the target cells in the present study.0.00,0.05,0.25,1.25 and 6.25 mg/ml nicotinamide were applied respectively.Western blot,fluorescent probes(Flu-3AM),flow cytometry analysis and time-lampse microscope digital skill were used to evaluate the effects of nicotinamide on melanosome motility and the melanosome distribution in melanocytes.Results The results showed that nicotinamide had a potential effect on regulating free calcium concentration in a dose-dependent manner(y=76.461 2-5.435x,r=0.97);The activity of Na+-K+-Ca2+-ATPase was down regulated with the increasing concentration of nicotinamide.The expression of cellular dynein was also altered by nicotinamide;Na +-K+-Ca2+-ATPase activity was kept normal when given 0.05,0.25 mg/ml nicotinamide,while the dynein protein expression was inhibited(P
