RESUMO
The purpose of this study was to investigate whether IgG, non-donor-specific anti-HLA class I antibodies (HLAabI) detected after renal transplantation recognize immunogenic amino acid triplets expressed on the foreign graft. In addition, we sought to evaluate the effect of these antibodies as well as other posttransplant HLAabI on graft outcome. Posttransplant sera from 264 renal recipients were tested for the presence of IgG HLAabI and HLA class II-specific alloantibodies (HLAabII) by ELISA. The HLAMatchmaker computer algorithm was used to define the HLA class I non-donor-specific antibodies, which seem to recognize immunogenic amino acid triplets. Donor-specific triplet antibodies (DSTRab) were detected in 16 of 22 (72.7%) recipients based on at least one HLA-A or -B mismatched antigen with the donor. DSTRab were found either without (n = 7) or with (n = 9) HLA donor-specific antibodies (HLA-DSA). The presence of DSTRab alone in the periphery was associated with acute rejection, whereas the presence of both DSTRab and HLA-DSA was associated with chronic rejection and graft failure.
Assuntos
Antígenos HLA-A/imunologia , Isoanticorpos/imunologia , Transplante de Rim/imunologia , Antígenos HLA-D/imunologia , Teste de Histocompatibilidade , Humanos , Imunoglobulina G/sangueRESUMO
Protein P1, which is a nuclear protein resembling high mobility group proteins, has been studied in human breast adenocarcinomas and compared to those from control tissue. The presence of the protein was confirmed by in vitro phosphorylation by casein kinase II and immunoblotting, using antibodies raised in rabbits against rat liver P1. The protein has been isolated by reverse phase HPLC chromatography which provides a more rapid method of purification requiring smaller amounts of material. The levels of P1 expression were investigated and it was found that there was a three-fold increase in the ratio of P1/histone H1 in normal breast tissue as compared to the neoplastic tissue. In two other malignant and non-malignant tissues studied, the level of P1 was also decreased in the malignant tissues. Thermolytic phosphopeptides of P1 from normal and malignant human breast tissues exhibited the same pattern, though when compared to the phosphopeptide pattern from rat tissue, differences were observed.
Assuntos
Adenocarcinoma/metabolismo , Neoplasias da Mama/metabolismo , Proteínas de Grupo de Alta Mobilidade/metabolismo , Proteínas Nucleares/metabolismo , Fatores de Transcrição , Aminoácidos/análise , Animais , Western Blotting , Caseína Quinase II , Proteínas de Ciclo Celular , Cromatografia Líquida de Alta Pressão/métodos , Proteínas de Ligação a DNA/metabolismo , Densitometria , Eletroforese em Gel de Poliacrilamida , Feminino , Células HeLa , Proteínas de Grupo de Alta Mobilidade/química , Proteínas de Grupo de Alta Mobilidade/isolamento & purificação , Histonas/análise , Humanos , Processamento de Imagem Assistida por Computador , Técnicas In Vitro , Componente 3 do Complexo de Manutenção de Minicromossomo , Peso Molecular , Proteínas Nucleares/química , Proteínas Nucleares/isolamento & purificação , Fosfopeptídeos/análise , Fosforilação/efeitos dos fármacos , Proteínas Serina-Treonina Quinases/metabolismo , CoelhosRESUMO
We purified a glycoprotein of molecular weight 50 kDa that has an N-terminal sequence similar to that of apolipoprotein H indicating that it is identical to or highly homologous to apolipoprotein H. There are indications that apolipoprotein H or its homologue may be involved in the fertilization process. Sperm motion was assessed employing computer-assisted semen analysis. The addition of the purified protein to prepared sperm samples from normospermic men increases significantly the straight line velocity (VSL) and the amplitude of lateral head displacement (ALH) but does not increase the number of progressively motile sperm.
