Insight Into Microbes and Plants Ability for Bioremediation of Heavy Metals.

Contamination of ground and surface water, soil, and air by harmful and carcinogenic chemicals is one of the most prevalent problems in the modern industrialized world. Heavy metal toxicity has demonstrated to be paramount hazardous and there are various risks associated with it. In addition, these heavy metals have adverse effects on human health and plant physiology. The field of bioremediation has undergone an impactful revolution in recent years due to an exponential increase in various issues related to soil and water pollution. Bioremediation is an advanced and efficient technology, which involves the use of biological means such as microorganisms and plants to degrade heavy metal contaminants. Among the millions of microbes present in the ecosystem, the highest metal adsorption ability is possessed by species belonging to genus Penicillium, Streptomyces, Bacillus, Rhizopus, Chlorella, Ascophyllum, Sargassum, and Aspergillus. Among different plant species, Allium, Eucalyptus, Helianthus, and Hibiscus are the main heavy metal absorbers. The present review concentrates on the research in the bioremediation of important heavy metals through the use of plants and microbes.

Elucidating micro RNAs role in different plant-pathogen interactions.

Bacteria, fungi, virus and nematode constitute the primary class of pathogens causing plant diseases. Plant-pathogen interactions are crucial for the identification of the host and pathogen and further establishments of a network of interaction that can cross regulate the gene expressions in both sides. After infection, the correct identification of pathogen through various molecular interactions elicit a defense response against the pathogen by alteration of gene expression by the host. Co-evolution of pathogen gives them the ability to counter the virulence response of the host and pathogen can also modulate the host gene expression pattern to make it more susceptible to the infection. Small non-coding RNA molecules (siRNAs and miRNAs) efficiently modulate gene expression at the transcriptional and post-transcriptional level and play a vital role in host defense. The pathogen can also use this double-sided sward in their defense by deregulating the plant immunity via transcriptional control of plant genes utilizing RNA interference or suppressing the host RNA interference response with the help of various RNA silencing suppressor proteins. This mini-review focused on the miRNAs involvement in host defense and how different families of these non-coding regulatory RNAs regulate the defense response against the pathogen.

Low back pain: critical assessment of various scales.

PURPOSE: To study the various pain assessment tools based on their psychometric properties and ease of use. METHODS: Published articles on psychometric properties of pain tools were accessed and data collected for low back pain (LBP)-specific tools, generic tools, neuropathic LBP tools, tools for cognitively impaired patients, and tools for acute LBP. RESULTS: Among the LBP-specific tools, Roland Morris Disability Questionnaire (RMDQ) and Oswestry Disability Index (ODI) have good construct validity and reliability, and responsiveness over short intervals. Quebec Back Pain Disability Scale (QBPDS) gauges only disability and sleep. Among the generic tools, McGill Pain Questionnaire (MPQ), West Haven-Yale Multidimensional Pain Inventory (MPI), and Brief Pain Inventory (BPI) show good responsiveness, but BPI is the only tool validated for LBP. Neuropathic Pain Scale (NPS) and Short Form-MPQ-2 (SF-MPQ-2) are both reliable tools for neuropathic LBP. For cognitively impaired patients, Pain Assessment in Advanced Dementia (PAINAD), Abbey Pain Scale (APS), and Doloplus-2 are all reliable tools, but PAINAD has good construct validity. For acute pain, Clinically Aligned Pain Assessment (CAPA) is reliable and responsive, but presently, unidimensional tools and SF-MPQ-2 are the tools most preferred. CONCLUSION: Based on psychometric properties and ease of use, the best tools for LBP seem to be RMDQ/ODI (among LBP-specific tools), BPI (among generic tools), SF-MPQ-2/NPS (for neuropathic LBP), PAINAD (for cognitively impaired patients), and unidimensional tools and SF-MPQ-2 (for acute pain). Overall, BPI seems to be a tool that can be relied upon the most. These slides can be retrieved under Electronic Supplementary Material.