DNA Interaction, in vitro Antibacterial and Cytotoxic Activities of Ru(III) Heterochelates.

Ruthenium(III) complexes [Ru(bphtpy)(PPh3)Cl3] (bphfpy = diphenylfuranylpyridine derivatives) were synthesized and characterized by LCMS, IR spectroscopy, elemental analysis and magnetic measurements. All the complexes were screened for their antibacterial activity in terms of minimum inhibitory concentration against two Gram-positive and three Gram-negative bacterial species. DNA binding study by absorption titration and viscosity measurement shows that complexes bind in an intercalating mode, which is also confirmed by molecular docking. All the complexes were also screened for the DNA nuclease property of pUC19 plasmid DNA. The cytotoxicity study of the synthesized complexes was performed to elucidate the LC50 values to find out the toxicity profile of the complexes.

Copper(II) Complexes with N,O-Donor Ligands and Ofloxacin Drug as Antibacterial, DNA Interacting, Cytotoxic and SOD Mimic Agent.

ABSTRACT: The N,O-donor bidentate ligands (L (1) -L (7) ) derived from the reaction between chalcones and pyridinium salt of 2-acetyl furan were synthesized and characterized by IR and NMR spectroscopic techniques. Their complexes [1-7] of Cu(II) were synthesized and characterized by elemental analysis, magnetic measurements, TG analyses, IR and mass spectroscopy. Synthesized complexes were carried out for their biological elucidation using different biological experiments like minimum inhibitory concentration, DNA binding and cleavage study, cytotoxicity, and antiradical activity. Efficient cleavage of pUC19 DNA was observed for all the test complexes than the reference drug. GRAPHICAL ABSTRACT: Increase in DNA chain length and hence the relative viscosity as the complexes binds to DNA via intercalative mode and involves a strong stacking interaction between an aromatic chromophore and the DNA base pair.

DNA interactions and cytotoxic studies of cis-platin analogues of substituted 2,2'-bipyridines.

Platinum(II) complexes [Pt(4″-fpbpy)Cl(2)] (1), [Pt(4″-mepbpy)Cl(2)] (2), [Pt(4″-mpbpy)Cl(2)] (3) and [Pt(4″-bopbpy)Cl(2)] (4) {where 4″-fpbpy=4-(4″-fluorophenyl)-6-phenyl-2,2'-bipyridine, 4″-mepbpy=4-(4″-methylphenyl)-6-phenyl-2,2'-bipyridine, 4″-mpbpy=4-(4″-methoxyphenyl)-6-phenyl-2,2'-bipyridine, 4″-bopbpy=4-(4″-benzyloxyphenyl)-6-phenyl-2,2'-bipyridine} have been synthesized and characterized. The binding strength and binding mode of the complexes with HS DNA (Herring Sperm) have been investigated by absorption titration and viscosity measurement studies. The results have been revealed that the complexes bind to DNA by covalent mode with intrinsic binding constant K(b) ranging from 6.05×10(4)M(-1) to 3.48×10(5)M(-1). The unwinding angle of pUC19 DNA has been evaluated by gel electrophoresis assay. The brine shrimp bioassay has been performed to study the in vitro cytotoxic properties of the synthesized metal complexes.