Characterization of host immunity during persistent vaginal colonization by Group B Streptococcus.

Streptococcus agalactiae (Group B Streptococcus, GBS) is a Gram-positive bacterium, which colonizes the vaginal tract in 10-30% of women. Colonization is transient in nature, and little is known about the host and bacterial factors controlling GBS persistence. Gaining insight into these factors is essential for developing therapeutics to limit maternal GBS carriage and prevent transmission to the susceptible newborn. In this work, we have used human cervical and vaginal epithelial cells, and our established mouse model of GBS vaginal colonization, to characterize key host factors that respond during GBS colonization. We identify a GBS strain that persists beyond a month in the murine vagina, whereas other strains are more readily cleared. Correspondingly, we have detected differential cytokine production in human cell lines after challenge with the persistent strain vs. other GBS strains. We also demonstrate that the persistent strain more readily invades cervical cells compared with vaginal cells, suggesting that GBS may potentially use the cervix as a reservoir to establish long-term colonization. Furthermore, we have identified interleukin-17 production in response to long-term colonization, which is associated with eventual clearance of GBS. We conclude that both GBS strain differences and concurrent host immune responses are crucial in modulating vaginal colonization.

Endurance markers are related with local neuromuscular response for the intact but not for the ACL reconstructed leg during high intensity running.

AIM: It has been demonstrated that the local neuromuscular response during high intensity exercise has a strong relationship with endurance markers. However, a diminished neuromuscular response has been reported for the operated leg in athletes having undergone anterior cruciate ligament reconstruction (ACLR). The purpose of the present study was to examine the relationships between endurance markers and the EMG response during high intensity running in ACLR athletes. METHODS: Fourteen ACLR soccer players underwent a GXT test to volitional exhaustion and a 10-min bout of high intensity running. During the 10-min bout, EMG data were recorded at the 3rd and 10th minute from the vastus lateralis bilaterally using a telemetric system. The final EMG levels were expressed as a percentage of the initial values. Pearson moment product correlations were used to assess the relationship between the endurance markers of VO2max, velocity at lactate threshold (vLT), velocity at 4mM (V4) and the final EMG levels. RESULTS: Final EMG levels for the intact leg had a very strong relationship with vLT (r=0.77, P=0.001) and a strong relationship with V4 (r=0.68, P=0.008). Final EMG levels for the reconstructed leg had moderate relationship with vLT (r=0.47, P=0.09) and V4 (r=0.52, P=0.06). CONCLUSION: The neuromuscular response of the intact leg during high intensity running shows strong to very strong relationships with endurance markers. Failure of the ACLR leg to present relationships of similar strength may indicate that chronic perturbations modify the ability of the local muscular environment to tolerate sustained high intensity efforts.

ACL injury and reconstruction: Clinical related in vivo biomechanics.

Several researchers including our group have shown that knee joint biomechanics are impaired after anterior cruciate ligament (ACL) injury, in terms of kinematics and neuromuscular control. Current ACL reconstruction techniques do not seem to fully restore these adaptations. Our research has demonstrated that after ACL reconstruction, excessive tibial rotation is still present in high-demanding activities that involve both anterior and rotational loading of the knee. These findings seem to persist regardless of the autograft selection for the ACL reconstruction. Our results also suggest an impairment of neuromuscular control after ACL reconstruction, although muscle strength may have been reinstated. These abnormal biomechanical patterns may lead to loading of cartilage areas, which are not commonly loaded in the healthy knee and longitudinally can lead to osteoarthritis. Muscle imbalance can also influence patients' optimal sports performance exposing them to increased possibility of knee re-injury. In this review, our recommendations point towards further experimental work with in vivo and in vitro studies, in order to assist in the development of new surgical procedures that could possibly replicate more closely the natural ACL anatomy and prevent future knee pathology.