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Mol Cell Proteomics ; 13(12): 3224-35, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25092905

RESUMO

The Chinese hamster ovary cell (CHO) is the major host cell factory for recombinant production of biological therapeutics primarily because of its "human-like" glycosylation features. CHO is used for production of several O-glycoprotein therapeutics including erythropoietin, coagulation factors, and chimeric receptor IgG1-Fc-fusion proteins, however, some O-glycoproteins are not produced efficiently in CHO. We have previously shown that the capacity for O-glycosylation of proteins can be one limiting parameter for production of active proteins in CHO. Although the capacity of CHO for biosynthesis of glycan structures (glycostructures) on glycoproteins are well established, our knowledge of the capacity of CHO cells for attaching GalNAc-type O-glycans to proteins (glycosites) is minimal. This type of O-glycosylation is one of the most abundant forms of glycosylation, and it is differentially regulated in cells by expression of a subset of homologous polypeptide GalNAc-transferases. Here, we have genetically engineered CHO cells to produce homogeneous truncated O-glycans, so-called SimpleCells, which enabled lectin enrichment of O-glycoproteins and characterization of the O-glycoproteome. We identified 738 O-glycoproteins (1548 O-glycosites) in cell lysates and secretomes providing the first comprehensive insight into the O-glycosylation capacity of CHO (http://glycomics.ku.dk/o-glycoproteome_db/).


Assuntos
Acetilgalactosamina/metabolismo , Eritropoetina/biossíntese , Glicoproteínas/química , N-Acetilgalactosaminiltransferases/metabolismo , Proteoma/química , Proteínas Recombinantes/biossíntese , Acetilgalactosamina/química , Sequência de Aminoácidos , Animais , Sequência de Bases , Células CHO , Engenharia Celular , Linhagem Celular , Cricetulus , Eritropoetina/genética , Expressão Gênica , Glicômica , Glicoproteínas/genética , Glicoproteínas/metabolismo , Glicosilação , Humanos , Isoenzimas/genética , Isoenzimas/metabolismo , Chaperonas Moleculares/antagonistas & inibidores , Chaperonas Moleculares/genética , Chaperonas Moleculares/metabolismo , Dados de Sequência Molecular , N-Acetilgalactosaminiltransferases/genética , Proteoma/genética , Proteoma/metabolismo , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , Proteínas Recombinantes/genética , Alinhamento de Sequência , Polipeptídeo N-Acetilgalactosaminiltransferase
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