The use of microalgae and their culture medium for biogas production in an integrated cycle.

Vinasse is a residue produced in large quantities as a sub-product of ethanol production. Anaerobic digestion of vinasse can yield large amounts of biogas, but often difficulties arise in maintaining stable operation, due to the acidity of the material (which has a pH between 3.5 and 5) and a strong tendency to further acidification. Anaerobically digested vinasse can be used as part of a culture medium for microalgae cultivation, for the production of biodiesel and other compounds, whilst the excess CO2 produced in the ethanol fermentation can be used to stimulate algal growth. During algae cultivation, the pH of the culture medium has a strong tendency to increase; therefore, recycling of the spent culture medium or the concentrated algae suspension to the anaerobic digester treating vinasse was considered an option for pH stabilization there. Batch tests, however, showed that alkalinity of the spent culture broth, in spite of its high pH, is too low (only 350 mgCaCO3L(-1)) to help stabilise the pH of vinasse digestion. Alkalinity of the algae suspension is higher and digestion of a mixture of vinasse and a suspension of algae results in efficient biogas production, but still the alkalinity is insufficient to stabilise the pH in a range suitable for methanogenic microorganisms; hence, the addition of additional alkalinity, for instance as sodium bicarbonate or urea, remains necessary.

Application of urea dosing for alkalinity supply during anaerobic digestion of vinasse.

Pushed by demand for renewable energy, the ethanol industry in Brazil is expanding. However, production of 1 m(3) of ethanol generates around 13 m(3) of liquid residues (vinasse), so this expansion results in an increasing need for a more adequate destination of these residues. Nowadays the vinasse is dispersed on the sugar cane fields in the practice of fertirrigation, but anaerobic digestion of this residue may be a better solution, additionally offering an alternative source of energy, able to complement hydroelectric power supply in the dry season. However, when trying to digest vinasse at reduced hydraulic retention times, complications arise from its strong tendency toward acidification, upsetting the fragile balance of transformations normally occurring under anaerobic conditions. For successful operation of an anaerobic treatment process with acceptable hydraulic residence times, increasing alkalinity levels inside the reactor is neces-sary. In the present work we show that pH regulation by means of urea dosing, in spite of the risk posed by ammonia toxicity towards methanogenic biomass, can be a viable alternative to avoid vinasse acidification. The ammonia formed in urea conversion remains in solution, rather than escaping to the biogas, and so its use as fertiliser can offset its cost of application in the process.

Design and configuration criteria for wetland systems treating greywater.

Design and configuration for wetlands treating greywater are usually based on literature data obtained from domestic wastewater operating wetlands. It is very important to determine proper criteria for design and configuration to provide efficiency and minimum maintenance, avoiding bad odour and clogging amongst others, ensuring the acceptance of householders. The aim of this work was to design a wetland system treating greywater for a household and determine whether the chosen criteria were appropriate. Some of the criteria taken into consideration for design and configuration were: quantitative and qualitative characteristics, desired removal of biochemical oxygen demand (BOD) and suspended solids (TSS), substrate and ornamental aspect of the system. The system was composed of a grease trap (kitchen), sedimentation tank, a horizontal flow constructed wetland (HF-CW), intermittent feeding system, and a vertical flow constructed wetland (VF-CW). The results showed that the suggested design and configuration were in accordance with the expected efficiency. Being a compact system, it was susceptible to peak flows, temporarily deteriorating the performance of the HF-CW. The hybrid system, however, showed to cope well with influent fluctuations. The overall performance of the system shows that the removal of turbidity, TSS, COD and BOD were over 88%, reaching 95% removal for both BOD and turbidity.

Phosphate inhibition on thermophilic acetoclastic methanogens: a warning.

The inhibitory effect of phosphate on acetoclastic-methanogens was investigated for three different thermophilic (55 degrees C) anaerobic consortia. When 70 mM of phosphate was tested, acetoclastic methanogens was completely inhibited in "Eerbeek" sludge which is dominated by Methanosaeta-like methanogens. For the "Hoogezand" sludge the specific methanogenic activity dropped by 79%, indicating that any of the acetate-consuming methanogens present in the sludge was more resistant to the phosphate applied. The results demonstrated that the inhibitory effect of phosphate may affect both methane production rate and final methane concentration and might also be time dependent. This study indicates that the degree of inhibition is species-dependent, and even more resistant species may be affected during long-term experiments. Such inhibition is a matter of concern for researchers since misleading conclusions might be taken from growth and specific methanogenic activity tests when considerable concentrations of phosphate buffer are used and no interference is expected.

Bicarbonate dosing: a tool to performance recovery of a thermophilic methanol-fed UASB reactor.

The thermophilic-anaerobic treatment of methanol-containing wastewater in an upflow anaerobic sludge blanket (UASB) reactor, was found to be quite sensitive to pH shocks, both acid and alkaline. The results of the recovery experiments of sludge exposed to an alkaline shock, indicated that the addition or deprivation of sodium bicarbonate (NaHCO3) in the medium, plays an important role in the competition of methanogens and (homo)acetogens for methanol. In addition, caution has to be taken when using NaHCO3 for buffering methanol-containing wastewaters, since its introduction in the system will favour (homo)acetogenesis when proper conditions are not established. Based on these results, a recovery strategy for methanogenesis was proposed where bicarbonate is supplied stepwise, and the reactor is operated in a batch mode. This strategy was found to be appropriate, i.e. the results revealed that the recovery of methanogenesis on methanol from a reactor upset or complete failure caused by pH shock is possible, even in systems where (homo)acetogens are outcompeting methanogens. The time and the number of feedings required will depend on the degree of deterioration of the sludge.

Pathways of methanol conversion in a thermophilic anaerobic (55 degrees C) sludge consortium.

The pathway of methanol conversion by a thermophilic anaerobic consortium was elucidated by recording the fate of carbon in the presence and absence of bicarbonate and specific inhibitors. Results indicated that about 50% of methanol was directly converted to methane by the methylotrophic methanogens and 50% via the intermediates H(2)/CO(2) and acetate. The deprivation of inorganic carbon species [Sigma(HCO(3)(-)+CO(2))] in a phosphate-buffered system reduced the rate of methanol conversion. This suggests that bicarbonate is required as an electron (H(2)) sink and as a co-substrate for the efficient and complete removal of the chemical oxygen demand. Nuclear magnetic resonance spectroscopy was used to investigate the route of methanol conversion to acetate in bicarbonate-sufficient and bicarbonate-depleted environments. The proportions of [1,2-(13)C]acetate, [1-(13)C]acetate and [2-(13)C]acetate were determined. Methanol was preferentially incorporated into the methyl group of acetate, whereas HCO(3)(-) was the preferred source of the carboxyl group. A small amount of the added H(13)CO(3)(-) was reduced to form the methyl group of acetate and a small amount of the added (13)CH(3)OH was oxidised and found in the carboxyl group of acetate when (13)CH(3)OH was converted. The recovery of [(13)C]carboxyl groups in acetate from (13)CH(3)OH was enhanced in bicarbonate-deprived medium. The small amount of label incorporated in the carboxyl group of acetate when (13)CH(3)OH was converted in the presence of bromoethanesulfonic acid indicates that methanol can be oxidised to CO(2 )prior to acetate formation. These results indicate that methanol is converted through a common pathway (acetyl-CoA), being on the one hand reduced to the methyl group of acetate and on the other hand oxidised to CO(2), with CO(2) being incorporated into the carboxyl group of acetate.

Start-up of a thermophilic methanol-fed UASB reactor: change in sludge characteristics.

Experiments were performed to study the change in sludge characteristics and sludge granulation during the start-up of a thermophilic methanol-fed upflow anaerobic sludge bed (UASB) reactor. The laboratory scale reactor, was inoculated with thermophilic granular sludge and operated at 55 degrees C over 130 days at organic loading rates (OLR) varying from 2.7 to 47 gCOD.L(-1).d(-1). Physical characterisation was performed for both the seed and the cultivated sludge. Results demonstrated that a good quality, well settleable granular sludge was cultivated and retained in the reactor, allowing an OLR of 47 gCOD.L.d(-1) with 93% of methanol removal, where 79% was converted into methane. Using a community analysis of the cultivated consortia, high numbers of rod-shaped hydrogenotrophic methanogens were enumerated. Biomass washout coincided with a high specific gas load, but was not detrimental to the system in the conditions tested.

Thermophilic anaerobic digestion of methanol in UASB reactor.

A 5.1 L laboratory scale upflow anaerobic sludge bed (UASB) reactor was operated at 55 degrees C over 130 days in order to investigate the feasibility of treating methanol-containing wastewater under thermophilic conditions, focussing on start-up and process stability. Batch assays were conducted to elucidate the most probable pathway for methanol conversion. The results demonstrated a good performance, with a chemical oxygen demand (COD) removal averaging 82% throughout the experiment. No significant VFA accumulation was detected in the effluent, even with bicarbonate concentration exceeding 20 mM. Acetate was the main component of the VFA at relatively low organic loading rates (OLR). At high OLR, the main components were propionate and butyrate. Reactor performance was hardly affected when the system was exposed to non-optimal conditions, i.e., temperature drop, overloading and no feeding. Good thermophilic granular sludge was retained in the reactor. Washout of biomass was not severe during the experiment. From the pathway analysis it could be concluded that indirect pathways play an important role in the methanol degradation by the cultivated consortia.