Aminoglycoside antibiotics: thin-layer chromatography, bioautographic detection and quantitative assay.

A sensitive method is described for the identification of aminoglycoside antibiotics, for the detection of agents exhibiting biological activity in the course of fermentation, isolation and purification, and for following their chemical conversion. In the multi-component nebramycin complex the ratio of the factors can be established by the quantitative assay described.

Metabolites of gentamicin-producing Micromonospora species I. Isolation and identification of metabolites.

From the cultural broth of a Micromonospora species 25 aminocyclitol antibiotics were isolated by repeated ion-exchange chromatographic processes. The main components of the metabolites were identified as gentamicin C1, C2, and C1a. The other previously reported gentamicin type antibiotics and some other degradation products including gentamicin A, B, B1, X2, sisomicin, garamine, gentamines etc., were identified by chemical, PMR, and mass spectroscopic studies. Besides these, seven new gentamicin type antibiotics were isolated and characterized.