Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Sci Rep ; 9(1): 6437, 2019 04 23.
Artigo em Inglês | MEDLINE | ID: mdl-31015543

RESUMO

Treatment of plant seeds with electromagnetic fields or non-thermal plasmas aims to take advantage of plant functional plasticity towards stimulation of plant agricultural performance. In this study, the effects of pre-sowing seed treatment using 200 Pa vacuum (7 min), 5.28 MHz radio-frequency cold plasma (CP -2, 5, and 7 min) and electromagnetic field (EMF -5, 10, 15 min) on seed germination kinetics, content of phytohormones, morphometric parameters of seedlings and leaf proteome were assessed. CP 7 min and EMF 15 min treatments caused 19-24% faster germination in vitro; germination in the substrate was accelerated by vacuum (9%) and EMF 15 min (17%). The stressors did not change the seed germination percentage, with exception of EMF 5 min treatment that caused a decrease by 7.5%. Meanwhile both CP 7 min and EMF 15 min treatments stimulated germination, but the EMF treatment resulted in higher weight of leaves. Stressor-specific changes in phytohormone balance were detected in seeds: vacuum treatment decreased zeatin amount by 39%; CP treatments substantially increased gibberellin content, but other effects strongly varied with the treatment duration; the abscisic acid content was reduced by 55-60% after the EMF treatment. Analysis of the proteome showed that short exposure of seeds to the EMF or CP induced a similar long-term effect on gene expression in leaves, mostly stimulating expression of proteins involved in photosynthetic processes and their regulation.


Assuntos
Campos Eletromagnéticos , Regulação da Expressão Gênica de Plantas/efeitos da radiação , Helianthus/genética , Folhas de Planta/genética , Proteínas de Plantas/genética , Plântula/genética , Sementes/efeitos da radiação , Ácido Abscísico/metabolismo , Ontologia Genética , Germinação/efeitos da radiação , Giberelinas/metabolismo , Helianthus/crescimento & desenvolvimento , Helianthus/metabolismo , Anotação de Sequência Molecular , Reguladores de Crescimento de Plantas/metabolismo , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Proteínas de Plantas/classificação , Proteínas de Plantas/metabolismo , Gases em Plasma , Proteoma/classificação , Proteoma/genética , Proteoma/metabolismo , Ondas de Rádio , Plântula/crescimento & desenvolvimento , Plântula/metabolismo , Sementes/genética , Sementes/crescimento & desenvolvimento , Sementes/metabolismo
2.
Bioelectromagnetics ; 37(8): 536-548, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27574980

RESUMO

Radiofrequency (5.28 MHz) electromagnetic radiation and low-temperature plasma were applied as short-term (2-15 min) seed treatments to two perennial woody plant species, including Smirnov's rhododendron (Rhododendron smirnowii Trautv.) and black mulberry (Morus nigra L.). Potential effects were evaluated using germination indices and morphometry. The results suggest that treatment with electromagnetic field stimulated germination of freshly harvested R. smirnowii seeds (increased germination percentage up to 70%), but reduced germination of fresh M. nigra seeds (by 24%). Treatment with low-temperature plasma negatively affected germination for R. smirnowii, and positively for M. nigra. The treatment-induced changes in germination depended on seed dormancy state. Longer-term observations revealed that the effects persisted for more than a year; however, even negative effects on germination came out as positive effects on plant morphometric traits over time. Treatments characterized as distressful based on changes in germination and seedling length increased growth of R. smirnowii after 13 months. Specific changes included stem and root branching, as well as increased leaf count and surface area. These findings imply that longer-term patterns of response to seed stressors may be complex, and therefore, commonly used stressor-effects estimates, such as germination rate or seedling morphology, may be insufficient for qualifying stress response. Bioelectromagnetics. 37:536-548, 2016. © 2016 Wiley Periodicals, Inc.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...