[Peculiarities of the ovary structure in the rat offspring developing under conditions of the removed thyroid gland of female rat with the thyroxine replacement therapy and external radiation exposure in utero].

The influence of a single external gamma-radiation at a dose of 1.0 Gy (dose rate 9.08 x 10(-4) Gy/sec) on the 15th day of gestation in case of the removed complex of thyroid and parathyroid glands (thyroidparathyroidectomy) on the first day of gestation, as well as introduction of thyroxin and CaC12 on the structure of offspring ovary in postnatal ontogenesis (30-day old animals) was studied. It has been shown that thyroidparathyroidectomy of a female mother rat with thyroxine replacement therapy and irradiation, as well as the combination of these factors disturb the structure of ovarian tissues of the offspring. A single external irradiation on the 15th day of embryogenesis causes death of a considerable part of primordial follicles in the offspring ovary and growth of follicular layers in the secondary follicles. Thyroidparathyroidectomy of female rat on the first day of gestation with thyroxine replacement therapy causes delay in the development of follicles in the ovary at the early stages of maturation of 30-day old animals. The radiosensitivity of the ovarian tissues of the offspring that has been developed under the combined effect of the factors studied increases and results in an almost full loss of pool cells in the ovary of infant rats.

[Development of rat embryos after prolonged irradiation of ovocytes at the stage of immature follicles]. / Razvitie zarodyshei krysy posle prolongirovannogo oblucheniia ovotsitov na stadii primordial'nykh follikulov.

It was shown that the prolonged low-dose irradiation (up to 0.6 Gy) of the immature ovocytes of young rats (21-28 days old) caused delay of development of preimplantation embryos after fertilization of ovicells.

[The dynamics of folliculogenesis in the ovary of the progeny of animals irradiated in embryogenesis and early postnatal life]. / Dinamika follikulogeneza v iaichnike potomstva ot obluchennykh v émbriogeneze i rannei postnatal'noi zhizni zhivotnykh.

The prolonged irradiation with 1.0 Gy dose in prenatal and early postnatal periods of development of rats leads to the disturbance in the rate of follicular growth, the reduction of their pool, mainly the pool of young forms of oocytes. Processes of degeneration and destruction occur and develop in the ovary of both the irradiated rats and their first progeny.

[Effect of single-dose external gamma-irradiation of 0.5 g on the development of rat's embryos]. / Vliianie odnokratnogo vneshnego gamma-oblucheniia v doze 0,5 Gr na razvitie zarodyshei krysy.

Primigravida 4-month rats were exposed to single whole-body external gamma-radiation (137Cs, 0.5 Gy) during organogenesis on days 9 and 15 of pregnancy. The embryotoxic effect of radiation was studied on the 20th day of pregnancy. The irradiation caused significant growth disorders characterized mainly by a nonuniform hypoplasia of fetus organs. This effect was most pronounced in fetuses irradiated on the 15th day of the intrauterine development.

[The significance of the mu- and delta-opiate receptors in realizing enkephalin action on the course of hypoxic hypoxia]. / Znachenie miu- i del'ta-opiatnykh retseptorov v realizatsii deistviia énkefalinov na techenie gipoksicheskoi gipoksii.

New enkephalins analogues have been synthesized. They are characterized by linear, cyclic and branched peptide chain. A relationship has been established between antihypoxic activity of opioid peptides an their interaction with opiate receptors. Compounds efficiently interacting with mu-receptors irrespective of delta-receptors affinity, promote longer survival of mice in hypoxia. The antihypoxic effect of opioids is proportional to their specificity to mu-receptors.

[The interrelationship of doses and the pharmacological effects of cardiac peptides]. / Vzaimosviaz' doz i farmakologicheskikh éffektov peptidov serdtsa.

Low-molecular weight peptides prepared according to an original procedure from a cattle heart possess a protecting effect on the myocardium during ischemia. Peptides in doses of 10(-7) g/ml and less improve the indexes of contractility function of the isolated heart after total ischemia and correct respiration warranting cardiomyocytes survival during hypoxia. However, in different models high doses of heart peptides (5 x 10(-6) g/ml and more) are shown to inhibit contractility, to stop respiration, the action of the peptides increasing with myocardial ischemia degree.

[Effect of a drug isolated from the heart on the bioenergetics of cardiomyocytes in hypoxia and ischemia]. / Vliianie preparata, vydelennogo iz serdtsa, na bioénergetiku kardiomiotsitov v usloviiakh gipoksii i ishemii.

It has been established that cordialin inhibits selectively succinic acid oxidation and intensifies the exudation of a NADH2 substrate which is energetically more advantageous, and in this way has a favorable effect on energy metabolism, evidence of which is increase of the ATP content in the myocardium. Cordialin correction of the biologic energy of cardiomyocytes in hypoxia and ischemia prevents the accumulation of lipid drops in the cells of the peri-infarction zone, which reflects the high intensity of the processes of fatty acids beta-oxidation. As a result of this glycolysis activation is unnecessary (which is confirmed by high glycogen content, both of a qualitative and quantitative character, in the myocardium and low concentrations of glucose and lactate in the blood) and, consequently, the sequelae of acidosis are prevented.

[Blood cyclic nucleotide content in acute regional ischemia of the extremities]. / Soderzhanie tsiklicheskikh nukleotidov v krovi pri ostroi regionarnoi ishemii konechnosti.

Fifty healthy males aged from 19 to 34 years were examined. Experiments were conducted on 30 male mice weighing 200-250 g. After 10- and 20-minute ischemia of the upper limb the concentration of cAMP and cGMP in its venous blood increases in direct proportion to the duration of ischemia. Increase of the level of cyclic nucleotides (CN) in the blood does not depend on hypersecretion of catecholamines. Increase of the CN level coincides in time with increase of blood CPK. The mechanisms of changes in the blood CN level in ischemia are discussed.

[The information value of diagnosing myocardial infarct by blood density at the prehospital stage]. / Informativnost' diagnostiki infarkta miokarda po plotnosti krovi na dogospital'nom étape.

Basing on the clinical evidence, a comparative study was made of a diagnostic significance of ECG, biochemical findings and blood density in myocardial infarction. Unlike the former two criteria, changes in blood density allowed the clinicians to detect irreversible lesions in the myocardium. The method has advantages in prehospital diagnosis of the infarction as its efficacy at the very onset of the disease is superior to that of ECG. In view of blood density diagnosis of myocardial infarction convenience for practical use, reproducibility, ability to provide information when routine modalities fail to determine the ischemic myocardial lesion, it can be introduced as an adjuvant diagnostic means for cardiologic and common emergency teams.

[Effects of a polypeptide drug on the state of energy metabolism of myocardial cells in hypoxic and ischemic conditions]. / Vliianie polipeptidnogo preparata na sostoianie énergeticheskogo metabolizma kletok miokarda, nakhodiashchikhsia v usloviiakh gipoksii i ishemii.

Cordialin, the agent extracted from the heart, is known to inhibit hyperoxidation of succinic acid, increasing NADH oxidation speed in suspension of cardiomyocytes in hypoxia. Cordialin presence in oxygenated cells' suspension oxidating succinate, doesn't change oxygen consumption speed. The results received may be a theoretical basis for cordialin utilization in therapy of myocardial diseases, associated with hypoxia and ischemia. Cordialin utilization may be recommended for the treatment of acute myocardial infarction and for prolongation of time-period for thrombolytic therapy, treatment of IMD, angina and other pathological states, in which oxygen transport disturbance to myocardium cells occurs.

[Effects of a cardiac peptide preparation on the myocardium in ischemia]. / Vliianie peptidnogo preparata iz serdtsa na miokard v usloviiakh ishemii.

Based on experimental studies the possibility of cordialin use in acute ischemia is being substantiated. On the first day of the animals' mortality and increased life duration of cells in ischemia zone, delaying the injury region expansion. But later in rats, that were given cordialin, slowing down of injury zone recovery and scar tissue formation was demonstrated. Cordialin use in early stages of myocardial infarction is suggested. In experiments on isolated heart cordialin is reported to decrease the intensity of processes of lipids' peroxide oxidation in intact and ischemic myocardium. But in reperfusion cordialin activates LPO, that is associated with heart contracting activity inhibition. The results of the study may serve as an experimental basis for cordialin use on the first day of MI development. Its further use needs the correction of its ability to slow down the processes of necrotic tissue recovery.

[A simple and informative method of diagnosing myocardial infarct, based on a determination of blood density]. / Prostoi i informativnyi metod diagnostiki infarkta miokarda, osnovannyi na opredelenii plotnosti krovi.

An increased density of blood revealed in myocardial infarction patients served the basis for a simple and highly informative method developed by the authors to early diagnose myocardial infarction when other methods are not sensitive. The high density of blood is registered upon placing a drop of blood into flakes with sodium chloride solution of different concentration.

[Ultrastructure of the peri-infarction zone during treatment of experimental myocardial infarct with cardiolin]. / Ul'trastruktura okoloinfarktnoi zony pri lechenii kardiolinom éksperimental'nogo infarkta miokarda.

Cardiolin therapy influence on the periinfarction zone cardiomyocyte ultrastructure, sarcolemma permeability for colloidal lantan, myocardium energy level were studied in 25 white noninbred rats with experimental myocardial infarction. 6 hours after coronary occlusion ultrastructural damage in treated rats was less pronounced and mitochondria energy efficiency ratio was higher than those in the control group. 24 hours after the occlusion the tendency to normalization of the cardiomyocyte damage in treated rat myocardium was demonstrated, while in non-treated animals ischemic damage becomes irreversible, this being confirmed by the test with colloidal lantan. Cardiolin therapy prevents the infarction zone expansion by decreasing ischemic damage of periinfarction cardiomyocytes and by stimulating regeneration processes in these cells.

[Ultrastructure of cardiomyocytes in the peri-infarct zone during the treatment of experimental myocardial infarct in rats using the hexapeptide dalargin]. / Ul'trastruktura kardiomiotsitov periinfarktnoi zony pri lechenii éksperimental'nogo infarkta miokarda u krys geksapeptidom dalarginom.

The experiments on white rats with induced myocardial infarction have studied the influence of dalargin on the infarction size and peri-infarction zone ultrastructure. 24 hours later the decrease in the infarction zone size was detected in rats who had received dalargin in a dose of 50 and 100 micrograms/kg. In the peri-infarction zone the increase in glycogen quantity, the lower degree of lipid infiltration, the increase in mitochondrial number and mitochondrial energy effectiveness coefficient were noted, as compared to control animals. Sarcolemma of cardiomyocytes from the peri-infarction zone in rats on dalargin was impermeable for colloidal lanthanum. The decrease in the infarction size under the effect of dalargin is explained by its influence on the survival of cardiomyocytes in the peri-infarction zone.

[Changes in the biochemical and morphological indices of structural disturbance in the peri-infarct area of the myocardium as affected by opioid peptides in an experiment]. / Izmenenie biokhimicheskikh i morfologicheskikh pokazatelei narusheniia struktury okoloinfarktnoi zony miokarda pod vliianiem opioidnykh peptidov v éksperimente.

The effect of opiate peptides (leu-enkephalin, met-enkephalin and beta-endorphin) as well as of enkephalin synthetic analogs (two tetrapeptides and dalargin hexapeptide) on the activity of blood enzymes CPK and LDG1, the scope of myocardial infarction and ultrastructure of periinfarction cardiomyocytes has been studied on 200 white outbred rats with simulated myocardial infarction. The endogenous opiate peptides and dalargin hexapeptide were found to significantly decrease CPK and LDG1 activity and to diminish the size of the infarction. The effect is related to the drugs influence on the survival of periinfarction cardiomyocytes that can be proved electron-microscopically and using colloid lanthanum test.